期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-021-22276-z
关键词
-
资金
- National Science Foundation (NSF) [1629214]
- Open Philanthropy Project Improving Protein Design Fund
- National Institute of General Medical Sciences [R01GM120553]
- National Institute of Allergy and Infectious Diseases [HHSN272201700059C]
- Pew Biomedical Scholars Award
- Burroughs Wellcome Fund
- University of Washington Arnold and Mabel Beckman cryo-EM center
- NIH Molecular Biology Training Grant [T32GM008268]
- Washington Research Foundation (WRF) Innovation fellowship
- US DOE BES Energy Frontier Research Center CSSAS (The Center for the Science of Synthesis Across Scales) at the University of Washington [DESC0019288]
- PHS NRSA from NIGMS [T32GM007270]
- Human Frontiers Science Program Long Term Fellowship
- DOE Office of Biological and Environmental Research
- National Institute of Health project ALS-ENABLE [P30 GM124169]
- NIH [P30GM124165, S10OD021527, OD019994, RR029300]
- DOE [DE-AC02-06CH11357]
- National Institutes of Health (NIH)
- Howard Hughes Medical Institute
- Office of Basic Energy Sciences
- US Department of Energy [DE-AC02-05CH11231]
- Simons Foundation [SF349247]
- NYSTAR
- NIH National Institute of General Medical Sciences [GM103310]
- Agouron Institute [F00316]
- High-End Instrumentation Grant [S10OD018483]
- Office of Science
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1629214] Funding Source: National Science Foundation
The study introduces a stepwise hierarchical approach using helical repeat and helical bundle proteins as building blocks for building complex, multi-component protein assemblies, and provides a detailed structural characterization of the resulting assemblies.
A systematic and robust approach to generating complex protein nanomaterials would have broad utility. We develop a hierarchical approach to designing multi-component protein assemblies from two classes of modular building blocks: designed helical repeat proteins (DHRs) and helical bundle oligomers (HBs). We first rigidly fuse DHRs to HBs to generate a large library of oligomeric building blocks. We then generate assemblies with cyclic, dihedral, and point group symmetries from these building blocks using architecture guided rigid helical fusion with new software named WORMS. X-ray crystallography and cryo-electron microscopy characterization show that the hierarchical design approach can accurately generate a wide range of assemblies, including a 43nm diameter icosahedral nanocage. The computational methods and building block sets described here provide a very general route to de novo designed protein nanomaterials. De novo design of self-assembling protein nanostructures and materials is of significant interest, however design of complex, multi-component assemblies is challenging. Here, the authors present a stepwise hierarchical approach to build such assemblies using helical repeat and helical bundle proteins as building blocks, and provide an in-depth structural characterization of the resulting assemblies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据