Article
Biology
Maria Pia Miglietta, Sarah Pruski
Summary: Sampling in multiple localities and molecular barcoding reveal the presence of local cryptic species in allopatry in wide-ranging nominal species. However, cryptic species in sympatry can easily be missed if they have different seasonality and require long-term frequent sampling for identification. This study investigates the presence of cryptic species within a temporal gradient and on a spatial scale in Hydrozoa using mitochondrial sequencing and finds a high number of sympatric cryptic species. The bi-dimensional cryptic diversity highlighted in this study is crucial for understanding evolution processes, biogeography, and biodiversity assessments in the sea.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Zoology
Dariusz Skarzynski, Adrian Smolis, Lubomir Kovac, David Porco
Summary: A new species, Ceratophysella stachi, from Denmark, Germany, Luxembourg, Norway, Poland, and Ukraine is described based on morphological data and DNA barcodes. It belongs to a small European group of species with unique characteristics in chaetotaxy, and differs from closely related species in the arrangement of setae on the thoracic terga.
Article
Plant Sciences
Jianli Wang, Zhenfei Yan, Peng Zhong, Zhongbao Shen, Guofeng Yang, Lichao Ma
Summary: This study evaluated the utility of four candidate DNA barcoding regions in seven genera of Gramineae. It found that matK and ITS showed the highest discriminatory power, while rbcL and trnL-F were less effective. The study also demonstrated the feasibility of establishing a technical system for rapid and reliable identification of these seven genera, and provided crucial reference for analyzing the genetic diversity of grass germplasm resources.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Zhi-Fang Liu, Hui Ma, Xiao-Yan Zhang, Xiu-Qin Ci, Lang Li, Jian-Lin Hu, Can-Yu Zhang, Jian-Hua Xiao, His-Wen Li, John G. Conran, Alex D. Twyford, Peter M. Hollingsworth, Jie Li
Summary: The aim of DNA barcoding is to accurately identify species. However, universal barcodes often fail to discriminate species in taxonomically complex groups. The development of assays that allow low-cost access to large numbers of nuclear markers is a major challenge in plant DNA barcoding.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Plant Sciences
Irene Bosmali, Georgios Lagiotis, Nadia Haider, Maslin Osathanunkul, Costas Biliaderis, Panagiotis Madesis
Summary: By analyzing DNA barcoding regions, this study identified 71 Vicia specimens of Eurasian descent. The trnL and ITS2 regions were effective in discriminating the analyzed species, while the rpoC1 region could not identify all selected species due to sequence conservation or missing sequences in GenBank. The combination of DNA barcoding regions is essential for accurate species discrimination within the Vicia genus.
Article
Chemistry, Multidisciplinary
Arun Richard Chandrasekaran, Molly MacIsaac, Javier Vilcapoma, Clinton H. Hansen, Darren Yang, Wesley P. Wong, Ken Halvorsen
Summary: Molecular biomarkers are crucial in clinical settings and research labs, with the challenge of detecting multiple biomarkers providing a comprehensive overview. Programmable DNA nanoswitches enable the multiplexed detection of up to 6 biomarkers simultaneously, showcasing the potential for diverse biomolecular detection in a single assay.
Article
Chemistry, Multidisciplinary
Marco Potowski, Verena B. K. Kunig, Lukas Eberlein, Alexandros Vakalopoulos, Stefan M. Kast, Andreas Brunschweiger
Summary: DNA-encoded compound libraries are widely used in small molecule screening, aiming to cover chemical space with diverse molecules. By substituting purines with 7-deazaadenine in DNA barcodes, stability and reactivity of the barcodes are enhanced for a wider range of reactions in library design.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Entomology
Zhuo Ma, Jinliang Ren, Runzhi Zhang
Summary: This study analyzed the genetic distance variation of species in the subfamily Entiminae to determine parameters for species identification. The results showed that there is no universal genetic distance threshold at the subfamily level, but a threshold of 9.18% can delimit more than 88% of Entiminae species.
Article
Zoology
Giovanni Timossi, Peter Huemer
Summary: The species Sattleria karsholti was re-examined in this study using recently collected material, museum vouchers, and DNA barcodes. The study confirmed the existence of two allopatric species, S. karsholti, from the Orobian Alps in Italy. The morphological and molecular data supported the distinction between the two species, although the characteristics of the females remain unknown.
Article
Plant Sciences
Girme Aoudumbar Ramesh, Deepu Mathew, K. Joseph John, V Ravisankar
Summary: This study utilized DNA barcoding to identify seven species of Momordica and determine their relationships. Analysis of matK gene sequences resulted in distinct barcodes that could differentiate the species. The study also confirmed M. cymbalaria as a member of the Momordica genus.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Agriculture, Dairy & Animal Science
Mohd Hairul Salleh, Yuzine Esa, Rozihan Mohamed
Summary: Technological advancements in molecular approaches, such as DNA barcoding, have allowed for a better understanding of the genetic divergence and characteristics of the Southern River Terrapin population in Malaysia. Through the analysis of COI DNA barcodes, 16 haplotypes were identified, suggesting potential cryptic species between the Northern and Southern River Terrapins.
Article
Plant Sciences
Jie Wang, Chao-Nan Fu, Zhi-Qiong Mo, Michael Moeller, Jun-Bo Yang, Zhi-Rong Zhang, De-Zhu Li, Lian-Ming Gao
Summary: The taxonomy and phylogenetic relationships within the genus Cephalotaxus have been controversial. This study evaluated the efficiency of the complete plastome as a super-barcode in species discrimination and phylogenetic resolution in Cephalotaxus. A new taxonomic classification was proposed based on the plastome phylogeny, which also resolved all interspecific relationships. The rps16 gene was identified as a specific barcode for discriminating Cephalotaxus species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Entomology
Chao Song, Le Wang, Teng Lei, Xin Qi
Summary: Through analysis of collected species and public sequences, it is found that DNA barcodes can successfully delimit Microtendipes species and there are deep intraspecific divergences in some species. Additionally, color patterns are confirmed to be important diagnostic characters. Therefore, this study identifies and describes five new species and provides an updated key to male adults of known Microtendipes species from China.
Article
Chemistry, Multidisciplinary
Victor Pan, Wen Wang, Ian Heaven, Tanxi Bai, Yongxin Cheng, Chunlai Chen, Yonggang Ke, Bryan Wei
Summary: The study presents a simple and robust method for multiplexed molecular probing using fluorescent DNA barcodes generated from hierarchically assembled DNA origami superstructures. Machine learning algorithm was successfully utilized for high precision processing and identification of barcodes. The increase in the number of combinations was achieved through various methods, showcasing the potential for applications in target nucleic acid sequence capture and simultaneous characterization of binding kinetics of multiple complementary nucleic acids.
Review
Biodiversity Conservation
Svante Martinsson, Christer Erseus
Summary: The article provides a historical overview of the methods and data used for species delimitation of clitellate worms from the mid-1970s to present. It also discusses the taxonomical treatment of cryptic species and recommends describing and naming them when possible, while also looking towards the future prospects and further development of the field.
Article
Plant Sciences
Simon Verlynde, Cyrille A. D'Haese, Gregory M. Plunkett, Murielle Simo-Droissart, Molly Edwards, Vincent Droissart, Tariq Stevart
PLANT SYSTEMATICS AND EVOLUTION
(2018)
Article
Entomology
C. Schneider, S. D. Zon, C. A. D'Haese
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2018)
Article
Entomology
Adrian Smolis, Penelope Greenslade
AUSTRAL ENTOMOLOGY
(2020)
Article
Zoology
Penelope Greenslade
Correction
Zoology
Eduardo Mateos, Penelope Greenslade
Article
Zoology
Eduardo Mateos, Penelope Greenslade
Summary: This study describes three new species in the subgenus Setogaster Salmon of the Australian genus Lepidocyrtus, which are widely distributed and numerically dominant in Australia, living in different habitats and regions, and are considered to be endemic species.
Article
Zoology
Penelope Greenslade, Wanda Maria Weiner
Article
Zoology
Bruno Cavalcante Bellini, Penelope Greenslade, Enrique Baquero, Rafael Jordana, Paolla Gabryelle Cavalcante De Souza
Summary: This study describes a new species of the Australian Orchesellidae family and revisits the genus Australotomurus. Additionally, updated diagnoses to all genera and species are presented, along with an identification key.
Article
Zoology
Nikolas Gioia Cipola, Penelope Greenslade
Summary: Two new species of Acanthocyrtus, Acanthocyrtus necropolitanus and A. pallidus, are described in this study. These two species resemble other congenerics with smooth prelabral chaetae and a unique dorsal chaetotaxy pattern, but they are distinguished from others by the presence of labral papillae, which is a newly discovered feature in the genus. An identification key for all species in the genus is provided. The description of these two Acanthocyrtus species now increases from six to eight and they are known to be found in southwestern Australia.
Article
Entomology
Penelope Greenslade, John Ireson
Summary: This study revises the onychiurid fauna in Australia, identifying six species belonging to different genera. These species are mostly introduced to Australia from the Northern Hemisphere, and are primarily found in agricultural and horticultural soils, home gardens, and worm beds. The study provides a dichotomous key along with updated names, descriptions, and distribution and ecology information for each species. It also discusses the pest status of onychiurids in relation to root damage in home gardens and field crops in Australia. The aim of the study is to offer an identification guide for field workers to determine the need for control methods.
AUSTRAL ENTOMOLOGY
(2022)
Article
Zoology
Mahmood Mehrafrooz Mayvan, Penelope Greenslade, Hussein Sadeghi-Namaghi
Summary: Based on literature sources, we have compiled a catalogue of the genera and species of springtails (Collembola) found in Iran, located in Southwest Asia. The catalogue includes 301 named species, with 286 described species in 109 genera from 20 families recorded in Iran. It also provides information on dubious species and genera with unknown species, as well as details on biology, geographical distribution, ecology, authorship records, and bibliographical data of Iranian Collembola.
Article
Zoology
Nikolas Gioia Cipola, Penelope Greenslade
Summary: A new species of Troglopedetes Joseph, called Troglopedetes eberhardi sp. nov., is described and illustrated in this article. It shares similarities with other species in terms of eye presence and the arrangement of chaetotaxy along the body, but differs in certain features such as head chaetotaxy, fourth abdominal segment, and an elongated unguis median tooth. This is the first record of Troglopedetes in Australia, adding to the current total of 37 nominal species, nine of which have eyes.
Article
Biology
Clement Schneider, Christian Woehle, Carola Greve, Cyrille A. D'Haese, Magnus Wolf, Michael Hiller, Axel Janke, Miklos Balint, Bruno Huettel
Summary: The novel ultra-low input protocol allows for high-quality genome sequencing from single specimens of minute, field-preserved metazoans, providing a new approach for biodiversity research. The genomes of two submillimeter Collembola species were successfully sequenced, demonstrating the feasibility and effectiveness of this method.