Discrimination of gallbladder stone employing Laser-Induced Breakdown Spectroscopy (LIBS) and Photoacoustic Spectroscopy (PAS)

Compositional analysis of gallstone samples has been carried out, using Laser-Induced Breakdown Spectroscopy (LIBS) and Photoacoustic Spectroscopy (PAS). Classification of gallstone has been made on the basis of intensities of the inorganic and organic constituents present in the LIBS spectra. A regression plot is drawn between LIBS spectral intensities of organic & inorganic elements and the stoichiometric ratio of Cholesterol, Bilirubin and Calcium Carbonate. Atomic lines of various elements, as well as molecular signatures of CaO Orange band, CN Violet band, and C-2 Swan band, are observed in LIBS spectra. The relative hardness of gallstones is estimated from the intensity ratio of ionic to neutral atomic lines of the species observed in LIBS spectra. PAS is used for detecting molecular constituents in the gallstones. Principal Component Analysis (PCA) is performed for the discrimination of gallstones. It is found that PAS data, in combination with LIBS provide a suitable method for the compositional analysis of gallstones. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

Compositional analysis of gallstone samples was conducted using LIBS and PAS, with regression plots for classification and observation of atomic and molecular features in the spectra. Relative hardness of gallstones was estimated from LIBS spectra, molecular constituents were detected using PAS, and discrimination was performed using PCA. The combination of PAS and LIBS was found to be a suitable method for gallstone compositional analysis.