Strategies for Constructing and Operating DNA Origami Linear Actuators

Linear actuators are ubiquitous components at all scales of engineering. DNA nanotechnology offers a unique opportunity for bottom-up assembly at the molecular scale, providing nanoscale precision with multiple methods for constructing and operating devices. In this paper, DNA origami linear actuators with up to 200 nm travel, based on a rail threading a topologically locked slider, are demonstrated. Two strategies, one- and two-pot assembly, are demonstrated whereby the two components are folded from one or two DNA scaffold strands, respectively. In order to control the position of the slider on the rail, the rail and the inside of the slider are decorated with single-stranded oligonucleotides with distinct sequences. Two positioning strategies, based on diffusion and capture of signaling strands, are used to link the slider reversibly to determined positions on the rail with high yield and precision. These machine components provide a basis for applications in molecular machinery and nanoscale manufacture including programmed chemical synthesis.

Automated summary

Linear actuators based on DNA origami are demonstrated in this paper, providing nanoscale precision through bottom-up assembly at the molecular scale. Two assembly strategies and two positioning strategies are used to control the position of the slider on the rail with high yield and precision. These components have potential applications in molecular machinery and nanoscale manufacture, including programmed chemical synthesis.