期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2100136118
关键词
Echinochloa colona; glyphosate resistance; ABC transporter; glyphosate exporter; plasma membrane
资金
- National Natural Science Foundation of China [31901905]
- Australian Grains Research and Development Corporation
- Natural Science Foundation of Hunan Province, China [2020JJ5238]
- China Agriculture Research System [CARS-16-E19]
- Scientific Research Fund of Hunan Provincial Education Department [19B254]
Glyphosate, the most widely used herbicide globally, has led to the evolution of resistance in many weed species. A study identified an ATP-binding cassette transporter, EcABCC8, that confers glyphosate resistance when expressed in transgenic rice. This transporter likely effluxes glyphosate in a plasma membrane-localized manner, reducing cellular glyphosate levels.
Glyphosate is the most widely used herbicide in world agriculture and for general vegetation control in a wide range of situations. Global and often intensive glyphosate selection of very large weedy plant populations has resulted in widespread glyphosate resistance evolution in populations of many weed species. Here, working with a glyphosate-resistant (GR) Echinochloa colona population that evolved in a Western Australia agricultural field, we identified an ATP-binding cassette (ABC) transporter (EcABCC8) that is consistently up-regulated in GR plants. When expressed in transgenic rice, this EcABCC8 transporter endowed glyphosate resistance. Equally, rice, maize, and soybean overexpressing the EcABCC8 ortholog genes were made resistant to glyphosate. Conversely, CRISPR/Cas9-mediated knockout of the EcABCC8 ortholog gene OsABCC8 increased rice susceptibility to glyphosate. Subcellular localization analysis and quantification of glyphosate cellular levels in treated ABCC8 transgenic rice plants and isolated leaf protoplasts as well as structural modeling support that EcABCC8 is likely a plasma membrane-localized transporter extruding cytoplasmic glyphosate to the apoplast, lowering the cellular glyphosate level. This is a report of a membrane transporter effluxing glyphosate in a GR plant species, and its function is likely conserved in crop plant species.
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