期刊
PLOS ONE
卷 16, 期 3, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0248007
关键词
-
资金
- National Vaccine Institute (NVI) [2563. 1/8]
- National Research Council of Thailand (NTCT)
- Emerging Infectious Diseases and Vaccines Cluster
- Ratchadapisek Sompoch Endowment Fund, Chulalongkorn University
- Ratchadapiseksompotch Fund, Faculty of Medicine, Chulalongkorn University [RAMF-29/63]
- Grants for Development of New Faculty Staff, Ratchadapiseksompote Endowment Fund [DNS 63_031_30_009_2]
The construction strategy of DNA vaccine candidates expressing full-length wild-type SARS-CoV-2 spike protein can induce high levels of specific binding IgG in mice. The full-length S antigen is more potent than the truncated spike (S1 or S2) in inducing neutralizing antibodies and robust T cell responses. This finding provides important insights for vaccine development.
More than 65 million people have been confirmed infection with SARS-CoV-2 and more than 1 million have died from COVID-19 and this pandemic remains critical worldwide. Effective vaccines are one of the most important strategies to limit the pandemic. Here, we report a construction strategy of DNA vaccine candidates expressing full length wild type SARS-CoV-2 spike (S) protein, S1 or S2 region and their immunogenicity in mice. All DNA vaccine constructs of pCMVkan-S, -S1 and -S2 induced high levels of specific binding IgG that showed a balance of IgG1/IgG2a response. However, only the sera from mice vaccinated with pCMKkan-S or -S1 DNA vaccines could inhibit viral RBD and ACE2 interaction. The highest neutralizing antibody (NAb) titer was found in pCMVkan-S group, followed by -S1, while -S2 showed the lowest PRNT50 titers. The geometric mean titers (GMTs) were 2,551, 1,005 and 291 for pCMVkan-S, -S1 and -S2, respectively. pCMVkan-S construct vaccine also induced the highest magnitude and breadth of T cells response. Analysis of IFN-gamma positive cells after stimulation with SARS-CoV-2 spike peptide pools were 2,991, 1,376 and 1,885 SFC/10(6) splenocytes for pCMVkan-S, -S1 and -S2, respectively. Our findings highlighted that full-length S antigen is more potent than the truncated spike (S1 or S2) in inducing of neutralizing antibody and robust T cell responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据