Is the aquatic macrophyte Crassula helmsii a genuine copper hyperaccumulator?

Aims The Australian native hemi-aquatic herb C. helmsii (Crassulaceae) is a copper (Cu) accumulator, tolerant to a wide range of climatic conditions and able to concentrate >9000 mu g Cu g(-1) in its living tissues. These characteristics suggest practical potential for decontamination of Cu-polluted mine waste waters using a phyto-extraction approach. The aim of this study was to investigate Cu uptake in C. helmsii at different Cu2+ concentrations in solution and to test for the effect of pH on the concentration of free Cu2+ and Cu accumulation in this species. Methods Different solutions were tested in acid (pH 4.0) and mildly acidic (pH 5.8) conditions at five different Cu2+ concentrations (ranging from 0 to 5 mg Cu L-1). The concentration of free Cu2+ was evaluated using Geochem-EZ software. The distribution of Cu in C. helmsii tissues was examined with micro-X-ray Fluorescence (mu XRF) elemental mapping and with Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy (SEM-EDS). Results The highest shoot Cu concentration in C. helmsii was 5870 mu g Cu g(-1) in the 5 mg Cu L-1 treatment. Copper bioaccumulation was positively correlated with the Cu2+ treatment in the different solutions. The mu XRF and SEM-EDS analysis revealed that roots accumulated higher Cu concentrations than the shoots. The concentration of free Cu2+ was shown to be dependent upon the type of Fe-chelator (DTPA, EDDHA, HBED) used in the solution due to the displacement of Fe3+ and complexation of Cu2+. Conclusions Crassula helmsii is highly tolerant to Cu2+ in solution at acidic and mildly acidic pH, and able to accumulate high Cu concentrations in its tissue. This confirms its potential for applications in Cu phyto-extraction of acidic mine effluent waters high in Cu2+.

Automated summary

Crassula helmsii is highly tolerant to Cu2+ in acidic and mildly acidic solutions, and can accumulate high Cu concentrations in its tissues. Copper bioaccumulation is positively correlated with Cu2+ treatment in different solutions, with roots accumulating higher concentrations than shoots. Free Cu2+ concentration is influenced by the type of Fe-chelator used in the solution.