期刊
PHOTOSYNTHESIS RESEARCH
卷 149, 期 1-2, 页码 93-105出版社
SPRINGER
DOI: 10.1007/s11120-021-00841-3
关键词
Singlet oxygen; Chlorophyll fluorescence imaging; Photoinhibition; Thylakoid membranes; Microalgae
资金
- ELKH Biological Research Center
- National Research, Development and Innovation Office [NKFIH K 116016, NKFIH FK 128977]
- Hungarian Academy of Sciences, MTA Premium Postdoctoral Research Program [PREMIUM-2017-38]
Singlet oxygen is a significant damaging agent produced during illumination, primarily affecting chlorophyll complexes and cellular constituents in photosynthetic organisms. The utilization of external photosensitizers is an effective approach to study the physiological role of singlet oxygen.
Singlet oxygen (O-1(2)) is an important damaging agent, which is produced during illumination by the interaction of the triplet excited state pigment molecules with molecular oxygen. In cells of photosynthetic organisms O-1(2) is formed primarily in chlorophyll containing complexes, and damages pigments, lipids, proteins and other cellular constituents in their environment. A useful approach to study the physiological role of O-1(2) is the utilization of external photosensitizers. In the present study, we employed a multiwell plate-based screening method in combination with chlorophyll fluorescence imaging to characterize the effect of externally produced O-1(2) on the photosynthetic activity of isolated thylakoid membranes and intact Chlorella sorokiniana cells. The results show that the external O-1(2) produced by the photosensitization reactions of Rose Bengal damages Photosystem II both in isolated thylakoid membranes and in intact cells in a concentration dependent manner indicating that O-1(2) plays a significant role in photodamage of Photosystem II.
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