Review
Clinical Neurology
C. M. Diaz-Caneja, MW State, R. J. Hagerman, S. Jacquemont, O. Marin, C. Bagni, D. Umbricht, E. Simonoff, F. de Andres-Trelles, A. Kaale, G. Pandina, B. Gomez-Mancilla, P. P. Wang, J. Cusak, S. Siafis, S. Leucht, M. Parellada, E. Loth, T. Charman, J. K. Buitelaar, D. Murphy, C. Arango
Summary: In the field of neurodevelopmental disorders, multidisciplinary translational consortia developing basic and clinical research concurrently, especially incorporating a developmental perspective into the drug development process, may help overcome current challenges in identifying and testing new treatments.
EUROPEAN NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Cell Biology
Zhenqin Wu, Bryant B. Chhun, Galina Popova, Syuan-Ming Guo, Chang N. Kim, Li-Hao Yeh, Tomasz Nowakowski, James Zou, Shalin B. Mehta
Summary: A cell's shape and motion are predictive of its function and pathology. However, automated analysis of cell morphology remains challenging, particularly for primary human cells that cannot be genetically labeled. To address this issue, researchers developed DynaMorph, a computational framework that combines quantitative live cell imaging with self-supervised learning to enable automated and quantitative analysis of cell morphodynamics.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Rachel C. Bandler, Ilaria Vitali, Ryan N. Delgado, May C. Ho, Elena Dvoretskova, Josue S. Ibarra Molinas, Paul W. Frazel, Maesoumeh Mohammadkhani, Robert Machold, Sophia Maedler, Shane A. Liddelow, Tomasz J. Nowakowski, Gord Fishell, Christian Mayer
Summary: In this study, clonal relationships and transcriptomic signatures during mouse forebrain development were tracked using massively parallel tagging of progenitors, revealing diverse types of GABAergic neurons sharing a common lineage. Divergence of GABAergic clones was found to occur during embryogenesis upon cell-cycle exit, suggesting that differentiation into subtypes is initiated as a lineage-dependent process at the progenitor cell level.
Article
Multidisciplinary Sciences
Ryan N. Delgado, Denise E. Allen, Matthew G. Keefe, Walter R. Mancia Leon, Ryan S. Ziffra, Elizabeth E. Crouch, Arturo Alvarez-Buylla, Tomasz J. Nowakowski
Summary: Studies have shown that human cortical progenitors are capable of generating both excitatory neurons and cortical interneurons, expanding our understanding of the origins of neuronal diversity in the human cortex. By utilizing a new cellular barcoding tool called STICR, researchers were able to trace the clonal lineage of human cortical progenitors and identify transcriptional similarities between cortically born GABAergic neurons and cortical interneurons born from the ganglionic eminences.
Article
Clinical Neurology
Ethan Winkler, David Wu, Eugene Gil, David McCoy, Kazim Narsinh, Zhengda Sun, Kerstin Mueller, Jayden Ross, Helen Kim, Shantel Weinsheimer, Mitchel Berger, Tomasz Nowakowski, Daniel Lim, Adib Abla, Daniel Cooke
Summary: This study used endoluminal biopsy and computational fluid dynamic modeling to investigate gene expression and blood flow-mediated transcriptional changes in patients with brain arteriovenous malformations (bAVMs). The results revealed similar gene expression profiles to tissues obtained through open surgery and identified potentially targetable abnormalities in the Ras-MAPK signaling pathway. This biopsy method could facilitate precision medicine approaches to bAVMs research.
Article
Multidisciplinary Sciences
Ethan A. Winkler, Chang N. Kim, Jayden M. Ross, Joseph H. Garcia, Eugene Gil, Irene Oh, Lindsay Q. Chen, David Wu, Joshua S. Catapano, Kunal Raygor, Kazim Narsinh, Helen Kim, Shantel Weinsheimer, Daniel L. Cooke, Brian P. Walcott, Michael T. Lawton, Nalin Gupta, Berislav Zlokovic, Edward F. Chang, Adib A. Abla, Daniel A. Lim, Tomasz J. Nowakowski
Summary: This study constructed a cell atlas of the adult human cerebrovasculature using transcriptomic data of cerebrovascular cells. The cellular and molecular perturbations in brain arteriovenous malformations were investigated, revealing abnormal vascular patterning and inflammation associated with the disease.
Article
Multidisciplinary Sciences
Mercedes F. Paredes, Cristina Mora, Quetzal Flores-Ramirez, Arantxa Cebrian-Silla, Ashley Del Dosso, Phil Larimer, Jiapei Chen, Gugene Kang, Susana Gonzalez Granero, Eric Garcia, Julia Chu, Ryan Delgado, Jennifer A. Cotter, Vivian Tang, Julien Spatazza, Kirsten Obernier, Jaime Ferrer Lozano, Maximo Vento, Julia Scott, Colin Studholme, Tomasz J. Nowakowski, Arnold R. Kriegstein, Michael C. Oldham, Andrea Hasenstaub, Jose Manuel Garcia-Verdugo, Arturo Alvarez-Buylla, Eric J. Huang
Summary: Research has revealed that the human embryonic ventral forebrain contains nests of proliferative neuroblasts which play a crucial role in generating a sufficient number of functional interneurons for the human brain.
Article
Multidisciplinary Sciences
Matthew T. Schmitz, Kadellyn Sandoval, Christopher P. Chen, Mohammed A. Mostajo-Radji, William W. Seeley, Tomasz J. Nowakowski, Chun Jimmie Ye, Mercedes F. Paredes, Alex A. Pollen
Summary: This study investigates the gene expression trajectories of inhibitory neurons (INs) in primates and mice during neurogenic period. Researchers find that while the initial classes of INs generated prenatally are largely conserved among mammals, there are contrasting developmental mechanisms for specifying evolutionarily novel cell types during prenatal development.
Review
Neurosciences
Helen Rankin Willsey, A. Jeremy Willsey, Belinda Wang, Matthew W. State
Summary: This article explores the genes associated with autism spectrum disorder (ASD) and discusses the use of convergent neuroscience approaches to understand the pathobiology of ASD.
NATURE REVIEWS NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Denise E. Allen, Kevin C. Donohue, Cathryn R. Cadwell, David Shin, Matthew G. Keefe, Vikaas S. Sohal, Tomasz J. Nowakowski
Summary: The progenitors of the human neocortex are located in the ventricular and outer subventricular zones. This study demonstrates that astrocytes derived from these zones populate distinct layers and exhibit morphological and molecular differences.
Article
Multidisciplinary Sciences
Diankun Yu, Tao Li, Jean-Christophe Delpech, Beika Zhu, Priya Kishore, Tatsuhiro Koshi, Rong Luo, Karishma J. B. Pratt, Galina Popova, Tomasz J. Nowakowski, Saul A. Villeda, Xianhua Piao
Summary: Parvalbumin-positive (PV+) interneurons are vital for circuit rhythm maintenance and their decrease has been linked to autism spectrum disorders. Animal studies have shown that maternal immune activation (MIA) can lead to reduced PV+ interneurons and autism-like behaviors. This study reveals that MIA down-regulates microglial Gpr56 expression, and its deletion mimics MIA-induced interneuron defects and autism-like behaviors. Additionally, elevated microglial tumor necrosis factor-alpha expression is implicated in impairing interneuron generation. Genetically restoring Gpr56 expression in microglia can improve PV+ interneuron deficits and autism-like behaviors in offspring. Therefore, microglial GPR56 plays an important role in PV+ interneuron development and is a significant target in MIA-induced neurodevelopmental disorders.
Review
Cell Biology
Tomasz J. Nowakowski, Sofie R. Salama
Summary: The cerebral cortex forms early in development and shows variations across mammals. Cerebral organoids, as an experimental platform, can recapitulate human brain development and be used to study regeneration, evolution, and neurological disease models.
Article
Computer Science, Information Systems
David F. Parks, Kateryna Voitiuk, Jinghui Geng, Matthew A. T. Elliott, Matthew G. Keefe, Erik A. Jung, Ash Robbins, Pierre Baudin, Victoria T. Ly, Nico Hawthorne, Dylan Yong, Sebastian E. Sanso, Nick Rezaee, Jess L. Seveston, Spencer T. Seiler, Rob Currie, Alex A. Pollen, Keith B. Hengen, Tomasz J. Nowakowski, Mohammed A. Mostajo-Radji, Sofie R. Salama, Mircea Teodorescu, David Haussler
Summary: The Internet of Things (IoT) is widely used in technology companies but rarely in biology experiments. However, it can greatly benefit cloud biology research through alarm notifications, automation, and real-time monitoring of experiments.
INTERNET OF THINGS
(2022)
Article
Computer Science, Information Systems
Pierre Baudin, Victoria T. Ly, Pattawong Pansodtee, Erik A. Jung, Robert Currie, Ryan Hoffman, Helen Rankin Willsey, Alex A. Pollen, Tomasz J. Nowakowski, David Haussler, Mohammed A. Mostajo-Radji, Sofie R. Salama, Mircea Teodorescu
Summary: The platform enables users to remotely monitor biological experiments with control and data analysis capabilities.
INTERNET OF THINGS
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
Danqing Zhu, Galina Schmunk, Tomasz Nowakowski, David V. Schaffer
