期刊
NATURE PHYSICS
卷 17, 期 7, 页码 850-+出版社
NATURE RESEARCH
DOI: 10.1038/s41567-021-01213-3
关键词
-
资金
- German Research Foundation (DFG) [TRR 174, 269423233, SFB1032, 201269156]
- Excellence Cluster ORIGINS under Germany's Excellence Strategy [EXC-2094-390783311]
- DFG fellowship through the Graduate School of Quantitative Biosciences Munich(QBM)
- research network MaxSynBio via German Federal Ministry of Education and Research (BMBF)
- research network MaxSynBio via Max Planck Society
This study reveals a hidden function of the Escherichia coli MinDE protein system in actively transporting functionally unrelated cargo on membranes through a diffusiophoretic mechanism. This mechanism enables the sorting of diffusive objects based on their effective size. The coupling between diffusive fluxes of MinDE and non-specific cargo via density-dependent friction represents a novel physical mechanism for establishing intracellular organization.
Protein oscillations linked to cell division in Escherichia coli are shown to localize unrelated molecules on the cell membrane via a diffusiophoretic mechanism, in which an effective friction fosters cargo transport along the fluxes set up by the proteins. The healthy growth and maintenance of a biological system depends on the precise spatial organization of molecules within the cell through the dissipation of energy. Reaction-diffusion mechanisms can facilitate this organization, as can directional cargo transport orchestrated by motor proteins, by relying on specific protein interactions. However, transport of material through the cell can also be achieved by active processes based on non-specific, purely physical mechanisms, a phenomenon that remains poorly explored. Here, using a combined experimental and theoretical approach, we discover and describe a hidden function of the Escherichia coli MinDE protein system: in addition to forming dynamic patterns, this system accomplishes the directional active transport of functionally unrelated cargo on membranes. Remarkably, this mechanism enables the sorting of diffusive objects according to their effective size, as evidenced using modular DNA origami-streptavidin nanostructures. We show that the diffusive fluxes of MinDE and non-specific cargo couple via density-dependent friction. This non-specific process constitutes a diffusiophoretic mechanism, as yet unknown in a cell biology setting. This nonlinear coupling between diffusive fluxes could represent a generic physical mechanism for establishing intracellular organization.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据