Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chairmandurai Aravindraja, Syam Jeepipalli, Krishna Mukesh Vekariya, Ruben Botello-Escalante, Edward K. L. Chan, Lakshmyya Kesavalu
Summary: This study aimed to investigate the global miRNA expression kinetics during the progression of periodontitis in mice infected with T. denticola. The results showed that T. denticola infection led to bacterial colonization and increased alveolar bone resorption. A total of 70 miRNAs with differential expression were identified, and miR-486, miR-126-3p, and miR-126-5p were found to be potential invasive biomarkers of T. denticola in periodontal disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiaomin Liu, Xianyi Wang, Binshu Chai, Zong Wu, Zhitao Gu, Heng Zou, Hui Zhang, Yanli li, Qiangling Sun, Wentao Fang, Zhongliang Ma
Summary: This study found that miR-199a-3p/5p is down-regulated in non-small cell lung cancer (NSCLC), and overexpression of miR-199a-3p/5p can inhibit cell proliferation, migration, and promote apoptosis. Rheb is identified as a common target of miR-199a-3p and miR-199a-5p, participating in the regulation of the mTOR signaling pathway. Additionally, miR-199a significantly inhibits tumor growth and metastasis in NSCLC and enhances the sensitivity of gefitinib in EGFR-T790M mutation. MiR-199a-3p/5p has the potential to serve as an early diagnostic marker or therapeutic target for NSCLC.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Giulia Accardi, Filippa Bono, Giuseppe Cammarata, Anna Aiello, Maria Trinidad Herrero, Riccardo Alessandro, Giuseppa Augello, Ciriaco Carru, Paolo Colomba, Maria Assunta Costa, Immaculata De Vivo, Mattia Emanuela Ligotti, Alessia Lo Curto, Rosa Passantino, Simona Taverna, Carmela Zizzo, Giovanni Duro, Calogero Caruso, Giuseppina Candore
Summary: This study found that the levels of four circulating miRNAs gradually increase with age, except for the ultra-centenarians who show levels comparable to young individuals. These miRNAs may serve as biomarkers of ageing and have diagnostic/prognostic relevance for age-related diseases.
Article
Nutrition & Dietetics
P. Kochhar, P. Dwarkanath, G. Ravikumar, A. Thomas, J. Crasta, T. Thomas, A. Kurpad, A. Mukhopadhyay
Summary: The study investigated the association of placental miRNAs (miR-21-5p, miR-141-3p, and miR-210-3p) expression with maternal, placental, and newborn parameters. The results suggest that miR-21-5p and miR-210-3p may be involved in placental growth, but not mediated through placental expression of PTEN, VEGF, FLT, or ENG.
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Microbiology
Ata Garajei, Milad Parvin, Hady Mohammadi, Abdolamir Allameh, Azin Hamidavi, Masoud Sadeghi, Azadeh Emami, Serge Brand
Summary: This study evaluated the expression of miR-486-3p, miR-561-5p, miR-548-3p, and miR-509-5p in tissue biopsy samples with and without OSCC. The results showed that the expression of miR-486-3p and miR-561-5p was significantly lower in cancer samples compared to normal tissue samples, while miR-548-3p expression increased in the OSCC group compared to the control group. There was no significant difference in miR-509-5p expression between OSCC and control groups.
Article
Health Care Sciences & Services
Paola Fuso, Mariantonietta Di Salvatore, Concetta Santonocito, Donatella Guarino, Chiara Autilio, Antonino Mule, Damiano Arciuolo, Antonina Rinninella, Flavio Mignone, Matteo Ramundo, Brunella Di Stefano, Armando Orlandi, Ettore Capoluongo, Nicola Nicolotti, Gianluca Franceschini, Alejandro Martin Sanchez, Giampaolo Tortora, Giovanni Scambia, Carlo Barone, Alessandra Cassano
Summary: The up-regulation of miRNAs let-7a-5p, mirR-100-5p, miR-101-3p, and miR-199a-3p is associated with better event-free survival and overall survival in breast cancer patients, regardless of breast cancer subtypes.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Oncology
Daniela Di Paolo, Francesca Pontis, Massimo Moro, Giovanni Centonze, Giulia Bertolini, Massimo Milione, Mavis Mensah, Miriam Segale, Ilaria Petraroia, Cristina Borzi, Paola Suatoni, Chiara Brignole, Patrizia Perri, Mirco Ponzoni, Ugo Pastorino, Gabriella Sozzi, Orazio Fortunato
Summary: Simultaneously targeting miR-126-3p and miR-221-3p to inhibit tumor growth and metastasis could be a novel therapeutic approach for lung cancer management.
MOLECULAR ONCOLOGY
(2021)
Article
Immunology
Qi Gao, Liming Zhang, Ruiqun Qi, Li Qiu, Xinghua Gao, Ting Xiao, Hongduo Chen
Summary: The study identified miR-126-3p and miR-16-5p as potential serum biomarkers for symptomatic dermographism (SD) with diagnostic capabilities. The two miRNAs were found to show an inverse correlation with serum level of VEGF-A, indicating their potential role in the pathogenesis of SD.
CLINICAL IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ovidiu Aghiorghiesei, Oana Zanoaga, Lajos Raduly, Alexandra Iulia Aghiorghiesei, Paul Chiroi, Andrada Trif, Rares Buiga, Liviuta Budisan, Ondine Lucaciu, Laura Ancuta Pop, Cornelia Braicu, Radu Campian, Ioana Berindan-Neagoe
Summary: Oral squamous cell carcinoma (OSCC), the sixth most common cancer worldwide, is often diagnosed at an advanced stage, leading to high mortality rates. In this study, we examined the expression levels of miR-21-5p, miR-93-5p, miR-200c-3p, miR-205-5p, H19, MALAT1, and BCL2 in OSCC tissues and normal adjacent tissues. Our results showed dysregulated expression of these transcripts, suggesting their potential as diagnostic markers for OSCC.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Omayma O. Abdelaleem, Shereen Rashad Mohammed, Hassan S. El Sayed, Sherin Khamis Hussein, Doaa Y. Ali, Mostafa Y. Abdelwahed, Sylvana N. Gaber, Nada F. Hemeda, Rehab G. Abd El-Hmid
Summary: This study found that serum levels of miR-34a-5p and miR-199a-3p were significantly reduced in neonates with sepsis, and they were associated with the severity of sepsis.
Article
Multidisciplinary Sciences
Dipuo D. Motshwari, Cindy George, Don M. Matshazi, Cecil J. Weale, Saarah F. G. Davids, Annalise E. Zemlin, Rajiv T. Erasmus, Andre P. Kengne, Tandi E. Matsha
Summary: This study reveals dysregulation of whole blood miRNAs (miR-126-3p, -30a-5p, -1299, and -182-5p) in individuals with chronic kidney disease (CKD) in South Africans of mixed-ancestry. These miRNAs are independently associated with increased risk of CKD. Further research is needed to determine their role in CKD risk screening in African populations.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Tamyres Mingorance Carvalho, Guillermo Ortiz Brasil, Tayana Schultz Jucoski, Douglas Adamoski, Rubens Silveira de Lima, Cleverton C. Spautz, Karina Furlan Anselmi, Patricia Midori Murobushi Ozawa, Iglenir Joao Cavalli, Jaqueline Carvalho de Oliveira, Daniela Fiori Gradia, Enilze Maria de Souza Fonseca Ribeiro
Summary: The study identified miR-320a and miR-4433b-5p as highly specific and sensitive biomarkers for distinguishing breast cancer from controls, regardless of sample source. In tissues, lower levels of miR-150-5p and higher levels of miR-142-5p were significant biomarkers. Combining these miRNAs in panels improved the ability to differentiate breast cancer patients from controls.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Maristella Canovai, Monica Evangelista, Alberto Mercatanti, Romina D'Aurizio, Letizia Pitto, Francesca Marrocolo, Valentina Casieri, Marco Pellegrini, Vincenzo Lionetti, Sergio Bracarda, Milena Rizzo
Summary: In this study, we found that miR-96-5p, miR-183-5p, and miR-210-3p released by DCT resistant PCa clones decrease the efficacy of DCT in PCa cells. These miRNAs were found to interact with genes including FOXO1, IGFBP3, PDCD4, PPP2CB, and INSIG1, affecting the sensitivity of DCT. Additionally, circulating miR-183-5p and miR-21-5p were identified as potential biomarkers for predicting DCT resistance in PCa patients.
CELL DEATH DISCOVERY
(2023)
Article
Anatomy & Morphology
Xiaoyu Zhou, Haitao Liu, Yingying Pang, Muqun Wang, Shengming Liu
Summary: This study found that miR-21-5p is highly expressed in lung cancer and is associated with poor prognosis. The UTMD transfection had a stronger effect on lung cancer cells compared to liposome transfection. By targeting BTG2, miR-21-5p promotes cell proliferation and migration while suppressing apoptosis in lung cancer cells.
Article
Biotechnology & Applied Microbiology
Rehab S. Abdul-Maksoud, Rania A. Fouad, Tamer G. Elsayed, Reda A. Ibrahem, Amani E. Badawi
JOURNAL OF GENE MEDICINE
(2020)
Article
Pediatrics
Nagwan Y. Saleh, Hesham M. Aboelghar, Sherif S. Salem, Reda A. Ibrahem, Fatma O. Khalil, Ahmed S. Abdelgawad, Asmaa A. Mahmoud
Summary: Most children with COVID-19 have a non-severe type, while those with severe type show higher levels of D-dimer, hypoxia, shock, and the need for mechanical ventilation.
Article
Pediatrics
Nagwan Yossery Saleh, Reda Abdel Latif Ibrahem, Amany Abdel hakim Saleh, Shimaa El shafey Soliman, Asmaa Abdel Sameea Mahmoud
Summary: The study aimed to assess the role of surfactant protein D (SP-D) in predicting the severity of community-acquired pneumonia (CAP) in pediatric patients. Results showed that SP-D levels were significantly higher in children with severe pneumonia, as determined by various severity scores, and were associated with indicators of CAP severity. SP-D could serve as a useful predictive biomarker for detecting CAP severity in hospitalized children.
PEDIATRIC RESEARCH
(2022)
Article
Genetics & Heredity
Mona S. Habieb, Nesreen G. Elhelbawy, Alshimaa M. Alhanafy, Mohammad G. Elhelbawy, Ahmed S. Alkelany, Amany M. Wahb
Summary: The study found a higher frequency of the A-variant of the BCHE rs1803274 gene in breast cancer patients, which is associated with a lower BChE activity and may represent a susceptibility factor for breast cancer development. Additionally, the rs1803274 variant is linked to certain tumor features, suggesting potential prognostic value.
Article
Genetics & Heredity
Maathir Kamel El-Shafie, Azza Mohamed Abdu Allah, Alshimaa Mahmoud Alhanafy, Sara Kamal Rizk, Mona Salah El-din Habieb
Summary: The study found an association between LOC643714 rs12922061 and breast cancer risk, while no significant association was found regarding TNRC9 rs3803662. Additionally, rs12922061 was related to menopausal status and nodal stage of the patients.
Article
Rheumatology
Dalia S. Saif, Reda Abdellatif Ibrahem, Mohamed A. Eltabl
Summary: This study found that muscle and peripheral nerve involvement are common problems after COVID-19 infection, regardless of symptoms. Risk factors include hospitalization and severe respiratory symptoms.
INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES
(2022)
Article
Genetics & Heredity
Naglaa S. Elabd, Shimaa E. Soliman, Moamena S. Elhamouly, Suzy F. Gohar, Ayman Elgamal, Mahmoud Magdy Alabassy, Haitham A. Soliman, Abdelnaser A. Gadallah, Osama D. Elbahr, Ghada Soliman, Amany A. Saleh
Summary: This study confirms the potential diagnostic roles of lncRNAs ASB16-AS1 and AFAP1-AS1 in colorectal cancer and highlights their significance in predicting the prognosis of colorectal cancer patients. Moreover, the expression levels of these lncRNAs in early-stage colorectal cancer patients may also impact the selection of treatment protocols.
APPLICATION OF CLINICAL GENETICS
(2022)
Article
Dermatology
Azza G. A. Farag, Heba M. Abdallah, Reda A. L. Ibrahem, Mustafa E. Elshaib, Wafaa A. Shehata
Summary: This study found that psoriatic patients had significantly higher levels of ghrelin and lower levels of apelin-36 in comparison to controls. Ghrelin was negatively correlated with psoriasis severity, while apelin-36 was negatively correlated with glycated hemoglobin levels and can predict prediabetes in psoriatic patients.
JOURNAL OF EGYPTIAN WOMENS DERMATOLOGICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Shimaa E. Soliman, Naglaa S. Elabd, Salah M. EL-Kousy, Mohamed F. Awad
Summary: The study highlights the potential diagnostic roles of miR-30a-5p and miR-182-5p in gastric cancer, with miR-30a-5p showing the highest diagnostic specificity and sensitivity. These miRNAs could provide simple, noninvasive biomarkers for predicting gastric cancer, with miR-30a-5p potentially being an independent predictor of GC.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Anatomy & Morphology
Marwa M. Dawoud, Khaled K-E Abdelaziz, Alshimaa M. Alhanafy, Manar S. E-d Ali, Enas Ab B. Elkhouly
Summary: The expression of NPY1R in breast cancer correlates with aggressive features such as metastasis, perineurial invasion, advanced stages, and poor prognostic index, suggesting a potential prognostic role in breast cancer. Non-nuclear expression of NPY1R appears to be more important in determining the prognosis of breast cancer.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Rabab Yasin, Ahmed Abdelhakim Kamel Gomaa, Tamer Ghazy, Shaimaa Abdelhamid Hassanein, Reda Abdel Latif Ibrahem, Mohamed Hossameldin Khalifa
Summary: This study assessed the clinical, radiographic, and laboratory findings of COVID-19 patients with HRCT follow-up after discharge, aiming to predict lung fibrosis in survivors. Factors such as age, CT severity, consolidation/crazy-paving scores, and ICU admission were identified as independent risk factors associated with post-COVID-19 fibrosis in survivors.
EGYPTIAN JOURNAL OF RADIOLOGY AND NUCLEAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Mahmood A. Al-Azzawi, Mohamed M. N. AboZaid, Reda Abdel Latif Ibrahem, Moustafa A. Sakr