Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

(2020) Victor M Corman et al.   Eurosurveillance

Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

(2020) Roujian Lu et al.   LANCET

Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)

(2020) Giuseppe Lippi et al.   CLINICAL CHEMISTRY AND LABORATORY MEDICINE

Authors’ response: SARS-CoV-2 detection by real-time RT-PCR

(2020) Victor M Corman et al.   Eurosurveillance

Letter to the editor: SARS-CoV-2 detection by real-time RT-PCR

(2020) Trestan Pillonel et al.   Eurosurveillance

Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens

(2020) Cyril Chik-Yan Yip et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit

(2020) Arun K. Nalla et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Rapid Identification of Candida Species in Candidemia Directly from Blood Samples Using Imperfect Match Probes

(2020) Yoshitsugu Higashi et al.   Scientific Reports

ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens

(2020) Tao Suo et al.   Emerging Microbes & Infections

Diagnosing COVID‐19 in the Emergency Department: A Scoping Review of Clinical Examinations, Laboratory Tests, Imaging Accuracy, and Biases

(2020) Christopher R. Carpenter et al.   ACADEMIC EMERGENCY MEDICINE

Target sequence heterogeneity causes the ‘hook effect’ in fluorescent dye-based quantitative PCR

(2020) Kristina Warton et al.   BIOTECHNIQUES

Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR

(2020) Myungsun Park et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

Interpret with caution: An evaluation of the commercial AusDiagnostics versus in-house developed assays for the detection of SARS-CoV-2 virus

(2020) H. Rahman et al.   JOURNAL OF CLINICAL VIROLOGY

Rapid SARS-CoV-2 whole-genome sequencing and analysis for informed public health decision-making in the Netherlands

(2020) Bas B. Oude Munnink et al.   NATURE MEDICINE

Pitfalls in SARS‐CoV‐2 PCR diagnostics

(2020) Kerstin Wernike et al.   Transboundary and Emerging Diseases

Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome

(2020) Kashif Aziz Khan et al.   Royal Society Open Science

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

(2020) Chantal B. F. Vogels et al.   Nature Microbiology

Genetic Variability in Probe Binding Regions Explains False Negative Results of a Molecular Assay for the Detection of Dengue Virus

(2016) Carmen Koo et al.   VECTOR-BORNE AND ZOONOTIC DISEASES

Single-Nucleotide Polymorphisms and Other Mismatches Reduce Performance of Quantitative PCR Assays

(2013) S. Lefever et al.   CLINICAL CHEMISTRY

Real-time PCR (qPCR) primer design using free online software

(2011) Brenda Thornton et al.   BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

Quantitative effects of position and type of single mismatch on single base primer extension

(2009) Jer-Horng Wu et al.   JOURNAL OF MICROBIOLOGICAL METHODS

The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5′ Nuclease Assay

(2009) Ralph Stadhouders et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Thermal equivalence of DNA duplexes for probe design

(2008) Gerald Weber et al.   JOURNAL OF PHYSICS-CONDENSED MATTER