期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 46, 期 5, 页码 1132-1141出版社
WILEY-BLACKWELL
DOI: 10.1002/eji.201545914
关键词
CD4 positive T lymphocytes; Costimulation; CD28 antigens; Mast cells; T-cell proliferation
类别
资金
- Dutch Arthritis Foundation
- Dutch Organization for Scientific Research
- Research Foundation Sole Mio
- Leiden Research Foundation (STROL)
- Centre for Medical Systems Biology (CMSB) within Netherlands Genomics Initiative (NGI)
- IMI JU [115142-2]
- European Union [223404]
- ReumaFonds [LLP-5] Funding Source: researchfish
Mast cells are innate immune cells usually residing in peripheral tissues, where they are likely to activate T-cell responses. Similar to other myeloid immune cells, mast cells can function as antigen-presenting cells. However, little is known about the capacity of human mast cells to costimulate CD4(+) T cells. Here, we studied the T-cell stimulatory potential of human mast cells. Peripheral blood derived mast cells were generated and cocultured with isolated CD4(+) T cells. In the presence of T-cell receptor triggering using anti-CD3, mast cells promoted strong proliferation of T cells, which was two-to fivefold stronger than the T-cell promoting capacity of monocytes. The interplay between mast cells and T cells was dependent on cell-cell contact, suggesting that costimulatory molecules on the mast cell surface are responsible for the effect. However, in contrast to monocytes, the T-cell costimulation by mast cells was independent of the classical costimulatory molecule CD28, or that of OX40L, ICOSL, or LIGHT. Our data show that mast cells can costimulate human CD4(+) T cells to induce strong T-cell proliferation, but that therapies aiming at disrupting the interaction of CD28 and B7 molecules do not inhibit mast cell mediated T-cell activation.
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