期刊
JOURNAL OF BIOMECHANICS
卷 121, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2021.110400
关键词
Brillouin microscopy; Interference microscopy; Metastasis; Cell modulus
资金
- National Cancer Institute [U01 CA202177, R33CA204582]
- National Science Foundation [CMMI 1929412]
During cancer metastasis, tumor cells undergo deformation to pass through narrow gaps in blood vessel walls. Studies using non-contact methods found that tumor cells and nuclei soften during transendothelial migration, with nuclear membranes remaining soft for at least 24 hours. These adjustments in mechanical properties facilitate tumor cell extravasation.
During cancer metastasis, tumor cells undergo significant deformation in order to traverse through endothelial cell junctions in the walls of blood vessels. As cells pass through narrow gaps, smaller than the nuclear diameter, the spatial configuration of chromatin must change along with the distribution of nuclear enzymes. Nuclear stiffness is an important determinant of the ability of cells to undergo transendothelial migration, yet no studies have been conducted to assess whether tumor cell cytoskeletal or nuclear stiffness changes during this critical process in order to facilitate passage. To address this question, we employed two non-contact methods, Brillouin confocal microscopy (BCM) and confocal reflectance quantitative phase microscopy (QPM), to track the changes in mechanical properties of live, transmigrating tumor cells in an in vitro collagen gel platform. Using these two imaging modalities to study transmigrating MDA-MB-231, A549, and A375 cells, we found that both the cells and their nuclei soften upon extravasation and that the nuclear membranes remain soft for at least 24 h. These new data suggest that tumor cells adjust their mechanical properties in order to facilitate extravasation. (c) 2021 Elsevier Ltd. All rights reserved.
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