Article
Immunology
Jieru Yang, Farrhana Firdaus, Armira Azuar, Zeinab G. Khalil, Nirmal Marasini, Robert J. Capon, Waleed M. Hussein, Istvan Toth, Mariusz Skwarczynski
Summary: Utilizing cell-penetrating peptides for delivering lipopeptide-based vaccines combined with multilamellar liposomes can efficiently trigger immune responses and generate high antibody titers with high opsonic activity against pathogenic GAS strains. This approach shows promising potential for liposome-based GAS vaccine development.
Article
Chemistry, Medicinal
Tru Van Nguyen, Kyung-Hwa Lee, Yongzhuo Huang, Meong Cheol Shin, Yoon Shin Park, Hangun Kim, Cheol Moon
Summary: In this study, TAT peptide was conjugated to human growth hormone (hGH) to enhance its permeability for topical application. TAT-hGH significantly increased cell proliferation and accelerated wound healing when topically applied. This study demonstrates TAT-hGH as a potential therapeutic drug for wound healing and provides a new method for enhancing the permeability of protein drugs.
Article
Chemistry, Physical
Yinglan Yu, Chang Zu, Dongsheng He, Yanan Li, Qinying Chen, Qian Chen, Huimin Wang, Ruijuan Wang, Birendra Chaurasiya, Jennica L. Zaro, Yiran Wang, Jiasheng Tu, Chunmeng Sun
Summary: Artemisinin, well known as an antimalarial drug, can also be used for inflammation and cancer treatment. Studies have shown that utilizing a reversibly activatable cell-penetrating peptide can enhance the concentration of artemisinin in tumors, leading to improved antitumor effects.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Mo'ath Yousef, Ildiko Szabo, Jozsef Muranyi, Francoise Illien, Dora Soltesz, Csaba Bato, Gabriella Toth, Gyula Batta, Peter Nagy, Sandrine Sagan, Zoltan Banoczi
Summary: This study demonstrates the preparation and investigation of efficient cell-penetrating peptides (CPPs) and explores the possibility of using unnatural aromatic amino acids to mimic the properties of tryptophan. These highly active CPPs have been successful in delivering antitumor drugs into specific cell lines.
Article
Pharmacology & Pharmacy
Philipp Uhl, Gzona Bajraktari-Sylejmani, Dominik Witzigmann, Cindy Bay, Stefan Zimmermann, Jurgen Burhenne, Johanna Weiss, Walter E. Haefeli, Max Sauter
Summary: Oral delivery of peptides is limited due to their instability and poor absorption. While medium-chain fatty acids have gained regulatory approval with low oral bioavailability, attempts to use nanocarriers for peptide delivery have not been successful so far. In this study, a nanocarrier formulation using liposomes decorated with cyclic cell-penetrating peptides is reported, which significantly improves oral bioavailability in Beagle dogs. This formulation achieves a considerable oral bioavailability of 3.9% with the glycopeptide vancomycin and increases the oral bioavailability of exenatide, a large linear peptide, by 20-fold in Beagle dogs.
ADVANCED THERAPEUTICS
(2023)
Article
Pharmacology & Pharmacy
Emma M. McErlean, Cian M. McCrudden, John W. McBride, Grace Cole, Vicky L. Kett, Tracy Robson, Nicholas J. Dunne, Helen O. McCarthy
Summary: RALA, a cationic amphipathic peptide, has shown promise as an efficient nucleic acid delivery system. Rational peptide design was used to create six amphipathic peptides with potential for nucleic acid delivery, but not all were successful in transfection, highlighting the importance of peptide design for cellular uptake and endosomal escape. Pep2 showed similar characteristics to RALA but had issues with toxicity and lack of pH-responsiveness, making RALA the superior choice for non-toxic gene delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Sergey V. Kravchenko, Pavel A. Domnin, Sergei Y. Grishin, Nikita A. Vershinin, Elena V. Gurina, Anastasiia A. Zakharova, Viacheslav N. Azev, Leila G. Mustaeva, Elena Y. Gorbunova, Margarita I. Kobyakova, Alexey K. Surin, Roman S. Fadeev, Olga S. Ostroumova, Svetlana A. Ermolaeva, Oxana V. Galzitskaya
Summary: Combining antimicrobial peptides with cell-penetrating peptides has potential in enhancing antimicrobial activity against various bacterial strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Keisuke Tsuchiya, Kanako Horikoshi, Minami Fujita, Motoharu Hirano, Maho Miyamoto, Hidetomo Yokoo, Yosuke Demizu
Summary: Researchers have developed hydrophobic CPPs based on the hydrophobic CPP TP10 by introducing an aliphatic carbon side chain. These stapled peptides maintained the hydrophobicity of TP10, enhanced helicity, and improved cell-penetrating efficiency. The stapled peptide F-3 with CF showed stable α-helical structure and highest cell-membrane permeability, while peptide F-4 exhibited remarkable stability in complex formation with pDNA for efficient intracellular delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Chi Huang, Zhenyu Han, Michael Evangelopoulos, Chad A. Mirkin
Summary: This study presents the design and evaluation of a new type of CRISPR SNA that has the potential to broaden the scope and impact of genome editing. By utilizing spherical nucleic acid structures, this SNA efficiently delivers editing components to cell membranes and tissues. The modified Cas9 protein core enhances cellular uptake, and additional modifications facilitate endosomal escape and maximize nuclear localization and editing efficiency.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Pharmacology & Pharmacy
Ildiko Szabo, Mo'ath Yousef, Dora Soltesz, Csaba Bato, Gabor Mezo, Zoltan Banoczi
Summary: Cell-penetrating peptides (CPPs) are a diverse group of molecules with promising potential for transporting compounds into cells, but face challenges such as non-specific internalization and rapid elimination from the body. Despite growing understanding of the mechanisms involved, predicting and designing the cell-penetrating properties of CPPs remains a complex task. Efforts are being made to modify well-known CPPs in order to overcome their limitations and enhance their internalization capabilities and penetration mechanisms.
Article
Cell Biology
Hannah C. Hymel, Alireza Rahnama, Olivia M. Sanchez, Dong Liu, Ted J. Gauthier, Adam T. Melvin
Summary: Cell-penetrating peptides (CPPs) are powerful tools for delivering impermeable cargoes into cells. This study found that the net charge of the cargo affects the uptake of CPP/cargo complexes, with positively charged cargoes enhancing uptake and neutral/negatively charged cargoes diminishing uptake. The length of the cargo did not significantly affect uptake.
Article
Chemistry, Multidisciplinary
Toni A. Pringle, Oliver Coleman, Akane Kawamura, James C. Knight
Summary: This study evaluated the cellular internalization properties of Herceptin-TAT conjugates with different degrees of TAT labeling. The conjugates showed enhanced cell membrane translocation and increased cellular internalization in high HER2 cells. This research provides a potential strategy for improving the efficacy of antibodies.
Review
Pharmacology & Pharmacy
Yingke Liu, Zhihe Zhao, Man Li
Summary: Cell penetrating peptides (CPPs), a class of short peptides capable of translocating drugs across cell membranes, have shown significant potential in drug delivery, particularly in the treatment of cancer, myocardial ischemia, ocular posterior segment disorders, etc. Recent research has focused on CPP-functionalized nano-drug delivery systems, highlighting their promising clinical translation.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Yoshiyuki Hakata, Kazuma Yamashita, Sonoko Hashimoto, Takashi Ohtsuki, Masaaki Miyazawa, Mizuki Kitamatsu
Summary: The connection between a functional peptide and a cell-penetrating peptide (CPP) using a heterodimeric coiled-coil as a molecular zipper can enhance the intracellular delivery and activity of the functional peptide. In this study, we investigated the optimal length of the K/E zipper for effective intracellular delivery and autophagy induction by conjugating an autophagy-inducing peptide (AIP) with CPP via heterodimeric coiled-coils consisting of 1 to 4 repeating units (K/E zipper; AIP-Kn and En-CPP). The results showed that K/E zippers with n = 3 and 4 formed stable hybrids and effectively induced autophagy, with the former being more intensive.
Article
Medicine, Research & Experimental
Marina Buyanova, Ashweta Sahni, Rui Yang, Amar Sarkar, Heba Salim, Dehua Pei
Summary: Cyclic cell-penetrating peptide 12 (CPP12) is highly efficient at delivering cargo molecules into mammalian cells. However, its efficiency is reduced at lower concentrations or in the presence of serum proteins. In this study, CPP12 analogs were prepared with varying hydrophobicity and evaluated for cellular entry. CPP12-2, with a substitution of L-3-benzothienylalanine (Bta) for L-2-naphthylalanine (Nal), showed up to 3.8-fold higher efficiency, especially at low concentrations, due to improved endosomal escape efficiency.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
