Article
Chemistry, Analytical
Yu Tao, Ke Yi, Haixia Wang, Kai Li, Mingqiang Li
Summary: In this study, a novel and sensitive fluorescent biosensor based on CRISPR-Cas12a enzymes and metal nanoclusters was developed for rapid and sensitive detection of HBV. The method showed higher sensitivity and faster fluorescence response compared to traditional methods, and it did not require sophisticated equipment or harsh reaction conditions. The practical application of this method in detecting HBV DNA in human serum samples was also demonstrated with satisfactory results.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Veterinary Sciences
Chao Qin, Jiajia Liu, Wenqi Zhu, Muchu Zeng, Ke Xu, Jinmei Ding, Hao Zhou, Jianshen Zhu, Yuqing Ke, Lai Yan Li, Gaoyuan Sheng, Zhuoru Li, Huaixi Luo, Shengyao Jiang, Kangchun Chen, Xianting Ding, He Meng
Summary: African swine fever virus (ASFV) is a major cause of worldwide agricultural loss, and conventional detection methods are time-consuming and instrument-dependent. In this study, a one-pot visual detection system for ASFV using CRISPR/Cas12a technology combined with LAMP or RPA was developed. The system allows rapid, sensitive, and specific detection of ASFV and can be observed by the naked eye, making it suitable for point-of-care testing.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Bo Yang, Zhengwang Shi, Yuan Ma, Lijuan Wang, Liyan Cao, Juncong Luo, Ying Wan, Rui Song, Yiyong Yan, Kehu Yuan, Hong Tian, Haixue Zheng
Summary: A method for the convenient, portable, low cost, highly sensitive and specific detection of African swine fever virus (ASFV) was established using a combined LAMP-CRISPR assay. The detection limit reached 7 copies/microliter of p72 gene per reaction with no cross-reactivity with other porcine viruses. The performance of this method showed good consistency with real-time qPCR tests, demonstrating a great potential for on-site monitoring of ASFV in the field.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Chemistry, Analytical
Yanju Chen, Siwenjie Qian, Jian Wu, Xiaoping Zhong, Meixia Wang, Jin-song He, Xiaoping Yu
Summary: In this study, a microchip-assisted detection platform was proposed for the detection of African swine fever virus (ASFV). The liquid transfer between different reaction chambers of the microchip is driven by the gravity of liquid solutions, eliminating the need for complex liquid transfer and instruments. The platform has the advantages of low cost, high efficiency, and easy operation, making it promising for popularization in low-resourced areas.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Physical
Yu Tao, Ke Yi, Haixia Wang, Hae-Won Kim, Kai Li, Xiang Zhu, Mingqiang Li
Summary: In this study, the researchers developed a colorimetric biosensor based on CRISPR-Cas12a technology for the detection of hepatitis B virus (HBV) DNA. The biosensor exhibited excellent sensitivity, specificity, and stability, making it suitable for resource-limited settings in developing countries.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Zhiyu Fan, Yuxia Mei, Jiawei Xing, Tian Chen, Di Hu, Hui Liu, Yingjun Li, Derui Liu, Zufeng Liu, Yunxiang Liang
Summary: In this study, a novel detection method for Ralstonia solanacearum (RS) based on loop-mediated isothermal amplification (LAMP) and CRISPR/Cas12a was developed. This method can accurately and rapidly detect RS, and has potential application for the detection of tomato bacterial wilt in southern China. The detection process is simple, fast, and does not require professional laboratory equipment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Chao Li, Xuan Chen, Renqiao Wen, Peng Ma, Kui Gu, Cui Li, Changyu Zhou, Changwei Lei, Yizhi Tang, Hongning Wang
Summary: The study developed an all-in-one, simple, rapid, ultrasensitive, ultraspecific, and instrument-free visual detection method for Campylobacter jejuni. Based on ICB capture, LAMP reaction, and CRISPR/Cas12a detection, the method could detect very low concentrations of C. jejuni within a short period of time.
Article
Biochemical Research Methods
Maria-Carmen Marques, Raul Ruiz, Roser Montagud-Martinez, Rosa Marquez-Costa, Sandra Albert, Pilar Domingo-Calap, Guillermo Rodrigo
Summary: The study used the CRISPR-Cas12a system to detect SARS-CoV-2 variants with the E484K mutation, identifying one infection with this mutation in fecal samples from hospitalized patients in Valencia, Spain. This suggests that CRISPR diagnostics could be a valuable tool for monitoring the spread of escape mutations in epidemiology.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Analytical
Ran Liu, Ying He, Tian Lan, Jingjing Zhang
Summary: In this study, a system integrating CRISPR-Cas12a sensors with a portable glucose meter was developed, based on the target-induced activation of the collateral cleavage activity of Cas12a. The system demonstrated portability, low cost, and easy integration with suitable Cas12a sensors to recognize multiple targets, paving the way for broader practical applications of CRISPR-based diagnostics.
Article
Virology
Mandeep Singh, Chitra Seetharam Misra, Gargi Bindal, Shyam Sunder Rangu, Devashish Rath
Summary: This study developed a Cas12a nuclease-based assay for detecting mpox virus. The assay showed sensitivity and specificity in distinguishing mpox virus from other related viruses.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Analytical
Bum Ju Park, Man Seong Park, Jae Myun Lee, Yoon Jae Song
Summary: A rapid and specific virus detection method using CRISPR-Cas system has been developed to detect IAV and IBV with high sensitivity and without cross-reactivity, allowing for quick and accurate diagnosis of influenza viruses.
Article
Agriculture, Dairy & Animal Science
Lei Lei, Fan Liao, Lei Tan, Deyong Duan, Yang Zhan, Naidong Wang, Yuge Wang, Xiaoye Peng, Kaixin Wang, Xiaojiu Huang, Yi Yang, Aibing Wang
Summary: In this study, a visual, rapid, low-cost, sensitive, specific, and portable nucleic acid detection method for PCV2 was established by combining LAMP with CRISPR/Cas12a. This method showed reliable results with a low detectable limit, no cross-reaction with other porcine viruses, and a high coincidence rate with qPCR detection. It lays the foundation for developing a PCV2 detection kit.
Article
Engineering, Environmental
Haorui Cao, Kang Mao, Fang Ran, Pengqi Xu, Yirong Zhao, Xiangyan Zhang, Hourong Zhou, Zhugen Yang, Hua Zhang, Guibin Jiang
Summary: A portable paper device based on CRISPR/Cas12a and RT-LAMP was developed for the detection of SARS-CoV-2 in wastewater, offering high sensitivity and specificity. The device enables simultaneous detection of the N, E, and S genes through different visualization pathways, and provides semi-quantitative analysis. This study demonstrates a promising point-of-use method for wastewater-based surveillance.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Food Science & Technology
Hua Liu, Xiuwen Hu, Haijuan Zeng, Chuan He, Fang Cheng, Xueming Tang, Jinbin Wang
Summary: In this study, a rapid gene detection system was developed based on LAMP-CRISPR-Cas12a-LFA, which allows for quick and accurate detection of specific genes in field crops with high reproducibility and sensitivity.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Chemistry, Analytical
Luping Deng, Jinyao Du, Xu Hun
Summary: A nanoprobe photoelectrochemical (PEC) assay combined with CRISPR/Cas12a was developed for nucleic acid detection. Ternary heterojunctions, nanoflower MoS2/WS2/g-C3N4, were synthesized by hydrothermal method. The PEC assay showed high sensitivity and selectivity, and provided a different detection pattern for other nucleic acids.
MICROCHEMICAL JOURNAL
(2023)
Review
Gastroenterology & Hepatology
Limin Yue, Rongguang Zhang, Shuaiyin Chen, Guangcai Duan
Summary: This meta-analysis study suggests an association between H. pylori infection and hypertension, indicating an increased risk of hypertension. H. pylori infection is also associated with elevated blood pressure levels.
DIGESTIVE DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Junwei Li, Hongwei Yu, Yuexia Wang, Bin Wang, Rongguang Zhang, Shuaiyin Chen, Guangcai Duan
Summary: There is a significant association between Helicobacter pylori infection and hepatic encephalopathy. The infection rate of H. pylori also increases with the severity of hepatic encephalopathy. Eradication of H. pylori has a protective effect on hepatic encephalopathy.
JOURNAL OF PUBLIC HEALTH
(2023)
Article
Virology
Tiantian Sun, Dong Li, Xinchen Dai, Caiyun Meng, Yi Li, Cheng Cheng, Wangquan Ji, Peiyu Zhu, Shuaiyin Chen, Haiyan Yang, Yuefei Jin, Weiguo Zhang, Guangcai Duan
Summary: Sudden onset of pulmonary edema is the leading cause of mortality in hand-foot-and-mouth disease (HFMD) patients, but its pathogenesis remains unclear. Research suggests that immunopathogenesis plays a role in the occurrence of pulmonary edema in severe HFMD patients. In mouse infection models, enterovirus (EV) infection was found to induce immune system disorder, reduce the numbers of pulmonary and circulatory immune cells, increase the numbers of lung neutrophils, myeloid-derived suppressor cells (MDSCs), and activated T cells, and lead to apoptosis of lung cells and degradation of tight junction proteins. These findings suggest that EV infection triggers a complex immune defense mechanism, causing dysregulation of innate and adaptive immune cells, release of cytokines and inflammatory factors, and lung barrier damage leading to pulmonary edema.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Wangquan Ji, Ling Tao, Dong Li, Peiyu Zhu, Yuexia Wang, Yu Zhang, Liang Zhang, Shuaiyin Chen, Haiyan Yang, Yuefei Jin, Guangcai Duan
Summary: This study established a mouse model for CVA19 infection via oral route and revealed the pathogenesis of diarrhea and encephalomyelitis caused by CVA19. It provides a useful tool for studying CVA19 pathogenesis and evaluating the efficacy of vaccines and antivirals.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Chemistry, Multidisciplinary
Jiahao Qiu, Xingzhong Guo, Wei Lei, Ronghua Ding, Yun Zhang, Hui Yang
Summary: Cellulose aerogel was prepared using NaOH/urea solution as solvent and gelation and drying process. The resulting aerogel showed a uniform macroporous structure.
Article
Microbiology
Jinzhao Long, Jiangfeng Zhang, Yanyan Xi, Jiaxue Zhao, Yuefei Jin, Haiyan Yang, Shuaiyin Chen, Guangcai Duan
Summary: This study investigates the role of CRISPR-positive plasmids in the spread of antibiotic resistance genes and virulence genes in the Klebsiella genus. It reveals that CRISPR-positive plasmids engage in fierce competition with other plasmid types, impacting the dynamics of plasmid transmission.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Ya Wang, Yanli Teng, Juan Geng, Jinzhao Long, Haiyan Yang, Guangcai Duan, Shuaiyin Chen
Summary: The study investigated the functional roles of Hfq in Shigella sonnei by constructing an hfq deletion mutant. The results showed that the hfq deletion mutant was more sensitive to antibiotics and had impaired virulence. Transcriptome analyses supported the phenotype of the hfq mutant and predicted eleven novel Hfq-dependent sRNAs potentially involved in the regulation of antibiotic resistance and/or virulence in S. sonnei.
RESEARCH IN MICROBIOLOGY
(2023)
Editorial Material
Immunology
Zijie Li, Wangquan Ji, Shuaiyin Chen, Guangcai Duan, Yuefei Jin
Review
Immunology
Yu Chen, Bowen Dai, Shujie Han, Guangcai Duan, Haiyan Yang, Shuaiyin Chen, Wangquan Ji, Yuefei Jin
Summary: Hand, foot, and mouth disease (HFMD) is a mild viral infection that poses a threat to global public health. Typical HFMD is characterized by a short febrile illness with a distinctive rash on the hands, feet, and mouth. However, atypical HFMD caused by a specific virus can present with different skin manifestations, making it challenging to diagnose and distinguish from other severe skin diseases. Recognizing the distinguishing features of atypical HFMD is crucial for accurate diagnosis and prompt treatment. A comprehensive overview of atypical HFMD is necessary to improve clinical strategies. This review aims to summarize clinical investigations and differential diagnostics to provide a scientific guide for timely diagnosis and prevention of serious complications.
Article
Immunology
Yu Zhang, Shuaiyin Chen, Tiantian Sun, Guangcai Duan, Haiyan Yang, Huifen Feng, Wenjie Jiang, Dong Li, Wangquan Ji, Peiyu Zhu, Yuefei Jin
Summary: Coxsackievirus A6 (CVA6) infection is associated with myocardial injury in hand, foot, and mouth disease (HFMD). Neutrophil infiltration is linked to the occurrence of myocardial injury. Depletion of neutrophils reduces cardiac enzymes, pathology in the heart, and inflammatory cytokine production. Clinical observations also support the role of neutrophils in myocardial injury.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Review
Immunology
Ping Zheng, Fang Liu, Jinzhao Long, Yuefei Jin, Shuaiyin Chen, Guangcai Duan, Haiyan Yang
Summary: Staphylococcus aureus is a significant human pathogen that can cause a wide range of diseases. Conventional mouse studies lack predictive ability due to the differences in immune systems between mice and humans. Humanized mice provide a promising model for studying human-specific virulence factors and the interaction mechanisms of S. aureus with humans.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Review
Biotechnology & Applied Microbiology
Yue Shen, Kai Hu, Mingzhu Yuan, Guangcai Duan, Yongjun Guo, Shuaiyin Chen
Summary: In vitro diagnosis (IVD) technology, such as CRISPR-Cas system, has become an important tool for disease diagnosis due to its high sensitivity and specificity in detecting pathogens or biomarkers at an early stage of disease. Scientists have been focused on improving CRISPR-based detection and on-site point-of-care testing (POCT) by developing extraction-free, amplification-free, quantitative, one-pot, and multiplexed methods. This review discusses the potential roles of these novel approaches and platforms in quantification, molecular diagnostics, and multiplexed detection, and highlights their significance in addressing real-world challenges like the ongoing COVID-19 pandemic.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Review
Cell Biology
Peiyu Zhu, Wangquan Ji, Dong Li, Zijie Li, Yu Chen, Bowen Dai, Shujie Han, Shuaiyin Chen, Yuefei Jin, Guangcai Duan
Summary: Hand-foot-and-mouth disease (HFMD) is a viral illness commonly seen in young children under 5 years of age, characterized by oral herpes and rashes on the hands and feet. It is important to note that HFMD may be associated with fatal neurological complications, but currently, there is no specific pharmaceutical intervention for HFMD.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Review
Immunology
Teng Zhang, Juan Geng, Yazhe Du, Haiyan Yang, Yuefei Jin, Shuaiyin Chen, Guangcai Duan
Summary: This study evaluated the immunogenicity and safety of two doses of influenza vaccine compared to a single dose in people living with HIV. The analysis of 14 studies with a total of 2436 participants showed that two doses of the vaccine resulted in higher seroprotection and seroconversion rates, as well as a better immunogenicity based on geometric mean titers. However, the risk of fever was increased by 3.42 times in HIV-positive individuals receiving two doses of the vaccine, while the risk of myalgia was reduced by a quarter. No serious vaccine-related adverse events were reported.
INTERNATIONAL JOURNAL OF STD & AIDS
(2023)
Article
Biotechnology & Applied Microbiology
Yanyan Xi, Jiaxue Zhao, Jiangfeng Zhang, Yuefei Jin, Haiyan Yang, Guangcai Duan, Shuaiyin Chen, Jinzhao Long
Summary: This study analyzed the characteristics of CRISPR/Cas systems using data from 487 K. variicola genomes. The results showed that approximately 21.56% of the genomes contained at least one confirmed CRISPR array. Additionally, three types of CRISPR/Cas systems were identified and spacer analysis revealed the significance of CRISPR/Cas systems in controlling horizontal gene transfer.
LETTERS IN APPLIED MICROBIOLOGY
(2023)