Review
Biochemistry & Molecular Biology
Shuting Guan, Li Zhao, Ruiyun Peng
Summary: Mitochondrial supercomplexes, consisting of enzyme complexes and electron carriers, play a crucial role in improving the efficiency of mitochondrial respiratory chain and maintaining cellular metabolism homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Xiaozhou Li, Jian Pan, Huiling Li, Guangdi Li, Bohao Liu, Xianming Tang, Xiangfeng Liu, Zhibiao He, Zhenyu Peng, Hongliang Zhang, Luxiang Wang, Yijian Li, Xudong Xiang, Xiangping Chai, Yunchang Yuan, Peilin Zheng, Dongshan Zhang
Summary: DsbA-L plays an important role in acute kidney injury (AKI) by upregulating the levels of VDACI, thereby driving the progression of AKI. The study demonstrates that DsbA-L interacts with specific regions of VDACI in the mitochondria, leading to renal cell apoptosis and mitochondrial injury. The findings are validated through mouse models and analysis of patient samples.
Article
Chemistry, Multidisciplinary
Jing Wu, Yun-ge Lou, Xu-le Yang, Rui Wang, Ran Zhang, Ji-ye Aa, Guang-ji Wang, Yuan Xie
Summary: This study found that ER stress plays a crucial role in the inhibition of P450s activity in NAFLD, and silybin regulates P450s activity by attenuating ER stress. Silybin can restore the activity of P450s in liver microsomes and may have potential benefits for the treatment of NAFLD.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Biochemistry & Molecular Biology
Fabio Marcheggiani, Patrick Orlando, Sonia Silvestri, Ilenia Cirilli, Antonella Riva, Giovanna Petrangolini, Francesca Orsini, Luca Tiano
Summary: In this study, the researchers compared the cellular uptake of Coenzyme Q(10) (CoQ(10)) in human dermal fibroblasts and murine skeletal muscle cells using lipoproteins enriched with different formulations of CoQ(10). The results showed that lipoproteins enriched with UBQ, a phytosome form of CoQ(10), had higher bioavailability compared to lipoproteins enriched with crystalline CoQ(10).
Article
Cell Biology
Zonghong Li, Ruiming Zhang, Xuewei Yin, Nana Li, Siyuan Cui, Teng Wang, Xing Tan, Mingyue Shen, Yun Guo, Jinxin Wang, Dadong Guo, Ruirong Xu
Summary: Realgar, a Chinese medicine containing arsenic, has been used to treat acute promyelocytic leukemia (APL) for over a thousand years. This study found that realgar can inhibit APL cell proliferation and induce cell death in a time- and dose-dependent manner. Realgar decreases ATP levels and induces cell cycle arrest. The Bcl-2/Bax/Cyt-C/AIF signaling pathway is involved in realgar-induced APL cell death.
Article
Chemistry, Analytical
Zhixia Yao, Yaosheng Liu, Yongxing Diao, Guangxing Hu, Yuhong Qian, Zhuang Li
Summary: The presented fluorometry assay allows for the quantitative detection of trypsin with good repeatability and selectivity, with detection and quantification limits of 0.15 and 0.50 μg/mL, respectively. Accurate results were obtained through the detection of trypsin in practical serum and urine samples.
Article
Biochemistry & Molecular Biology
Nahid Shahabadi, Saba Zendehcheshm
Summary: This research investigated the biological activity of COX-AgNPs in simulated physiological conditions, specifically examining the structural changes in HHb and Cyt c proteins. The results showed that COX-AgNPs can bind to these proteins without altering their secondary structure.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Genetics & Heredity
Alessandra Torraco, Silvia Morlino, Teresa Rizza, Michela Di Nottia, Giorgia Bottaro, Luigi Bisceglia, Arianna Montanari, Marco Cappa, Marco Castori, Enrico Bertini, Rosalba Carrozzo
Summary: This study describes a mitochondrial disease caused by a mutation in the COX5A gene, resulting in symptoms such as failure to thrive, lactic acidosis, hypoglycemia, and short stature. The observed metabolic derangement in the girl is less severe compared to previously reported cases, with no involvement of the cardiovascular system and neurodevelopment.
Article
Biochemistry & Molecular Biology
Zhi He, Qiqi Chen, Liang He, Jinxin Xiong, Kuo Gao, Bolin Lai, Li Zheng, Yong Pu, Yuanyuan Jiao, Zhijun Ma, Ziting Tang, Mingwang Zhang, Deying Yang, Taiming Yan
Summary: This study revealed the importance of the mitochondrial apoptotic pathway in sex change in white eels, showing that apoptosis rate significantly increased and then decreased during the sex change process, suggesting that the mitochondrial apoptotic pathway may play a more important role than other apoptotic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ardeshir Afshar Mazandaran, Parvin Khodarahmi
Summary: Exposure to lead can affect the expression of the MT3 gene and alter the lipid profile in the body, but the addition of the adjuvant antioxidant CoQ10 can alleviate these negative effects.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Environmental Sciences
Zhuanxu Ouyang, Bijing Yang, Jiangnan Yi, Shanshan Zhu, Suge Lu, Yingwei Liu, Yangwei Li, Yuanliang Li, Khalid Mehmood, Riaz Hussain, Muhammad Ijaz, Jianying Guo, Zhaoxin Tang, Ying Li, Hui Zhang
Summary: This study revealed the harmful effects of sodium fluoride on liver tissues of ducks, including promoting apoptosis and altering the expression levels of key genes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Carmine Staiano, Laura Garcia-Corzo, David Mantle, Nadia Turton, Lauren E. Millichap, Gloria Brea-Calvo, Iain Hargreaves
Summary: Coenzyme Q (CoQ or CoQ(10)) was initially believed to be important only for mitochondrial respiratory chain, but recent studies show its essential role in various redox processes within different cellular membrane types. Endogenous CoQ biosynthesis is crucial for cellular function, and defects in this pathway lead to primary CoQ deficiencies, characterized by decreased tissue CoQ levels and subsequent functional defects. Secondary CoQ deficiencies can also arise from various cellular dysfunctions unrelated to biosynthesis. This article reviews the current understanding of CoQ biosynthesis, the defects causing diminished CoQ(10) levels in human tissues, and their clinical manifestations.
Article
Entomology
Shihui Long, Wenxin Cao, Yongyu Qiu, Ruohan Deng, Jiali Liu, Lidan Zhang, Renke Dong, Fengxin Liu, Sheng Li, Haigang Zhao, Na Li, Kang Li
Summary: Apoptosis is a crucial process for organism development, and our study reveals the presence of cytoplasmic cytochrome C during Drosophila salivary gland degradation, indicating a partially conserved mechanism in apoptosis between insects and mammals. The upregulation of mitochondria apoptosis factors, cytochrome C, and death-associated APAF1-related killer encoding genes, as well as the colocation of cytochrome C with cytoplasmic mitochondria marker mito-GFP, suggest the involvement of mitochondrial pathway in apoptosis. Furthermore, the inhibition or promotion of the transcription factor E93 affects the staining of cytochrome C and active caspase 3, and the mitochondrial membrane potential.
Article
Biochemistry & Molecular Biology
Yue Hu, Yao-Dong Dong, Yan-Chao Wu, Qiu-Xu Wang, Xiang Nan, Da-Li Wang
Summary: The study investigated the protective effects of AMPK inhibitor BML-275 on cerebral ischemia/reperfusion injury and found that BML-275 could alleviate cerebral infarction, neurological deficit, and neuronal apoptosis induced by occlusion. The inhibitor also induced anti-apoptosis and decreased the expression of apoptosis markers, suggesting that anti-apoptosis may be a potential neuroprotective strategy for stroke treatment.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ronald Jemmerson, Katherine Staskus, LeeAnn Higgins, Kathleen Conklin, Ameeta Kelekar
Summary: Intracellular LRG1 protects against apoptosis by raising the threshold of cytoplasmic Cyt c required to induce apoptosis and preventing the onset of the intrinsic pathway. This survival mechanism is likely distinct from its extracellular survival function reported by several research groups.
Article
Biochemistry & Molecular Biology
Pablo Hernansanz-Agustin, Jose Antonio Enriquez
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Cardiac & Cardiovascular Systems
Ana Victoria Lechuga-Vieco, Ana Latorre-Pellicer, Enrique Calvo, Carlos Torroja, Juan Pellico, Rebeca Acin-Perez, Maria Luisa Garcia-Gil, Arnoldo Santos, Navratan Bagwan, Elena Bonzon-Kulichenko, Ricardo Magni, Marina Benito, Raquel Justo-Mendez, Anna Katharina Simon, Fatima Sanchez-Cabo, Jesus Vazquez, Jesus Ruiz-Cabello, Jose Antonio Enriquez
Summary: This study demonstrates that divergent nonpathologic mtDNA heteroplasmy (DNPH) impairs mitochondrial function in critical tissues such as the heart and skeletal muscle, leading to severe pathology in adulthood. It suggests that potential incompatibilities between donor and recipient mtDNA should be considered in medical interventions.
Article
Chemistry, Multidisciplinary
Susana Carregal-Romero, Ana Beatriz Miguel-Coello, Lydia Martinez-Parra, Yolanda Marti-Mateo, Pablo Hernansanz-Agustin, Yilian Fernandez-Afonso, Sandra Plaza-Garcia, Lucia Gutierrez, Maria del Mar Munoz-Hernandez, Juliana Carrillo-Romero, Marina Pinol-Cancer, Pierre Lecante, Zurine Blasco-Iturri, Lucia Fadon, Ana C. Almansa-Garcia, Marco Moller, Dorleta Otaegui, Jose Antonio Enriquez, Hugo Groult, Jesus Ruiz-Cabello
Summary: Manganese ferrite nanoparticles have unique properties in bioimaging and catalytic therapies. They have been used as contrast agents in magnetic resonance imaging (MRI) and as catalase-mimicking nanozymes for hypoxia alleviation. A cost-efficient synthetic microwave method has been developed to manufacture ultrasmall manganese ferrite nanoparticles with enhanced bioimaging and catalytic properties. These nanoparticles can be used as multimodal contrast agents in MRI and positron emission tomography (PET), and also have improved catalytic efficiency.
Article
Multidisciplinary Sciences
Pablo Hernansanz-Agustin, Jose Antonio Enriquez
Summary: This article presents a protocol for measuring the activities of NADH-cyt c oxidoreductase and succinate-cyt c oxidoreductase in the presence of NaCl or KCl. The results show that Na+ decreases the fluidity of the inner mitochondrial membrane (IMM) and the transfer of ubiquinone (CoQ), and that the transfer of CoQ between complex I and III is functionally different from the transfer between complex II and III.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Alba Gonzalez-Franquesa, Pau Gama-Perez, Marta Kulis, Karolina Szczepanowska, Norma Dahdah, Sonia Moreno-Gomez, Ana Latorre-Pellicer, Rebeca Fernandez-Ruiz, Antoni Aguilar-Mogas, Anne Hoffman, Erika Monelli, Sara Samino, Joan Miro-Blanch, Gregor Oemer, Xavier Duran, Estrella Sanchez-Rebordelo, Marc Schneeberger, Merce Obach, Joel Montane, Giancarlo Castellano, Vicente Chapaprieta, Wenfei Sun, Lourdes Navarro, Ignacio Prieto, Carlos Castano, Anna Novials, Ramon Gomis, Maria Monsalve, Marc Claret, Mariona Graupera, Guadalupe Soria, Christian Wolfrum, Joan Vendrell, Sonia Fernandez-Veledo, Jose Antonio Enriquez, Angel Carracedo, Jose Carlos Perales, Ruben Nogueiras, Laura Herrero, Aleksandra Trifunovic, Markus A. Keller, Oscar Yanes, Marta Sales-Pardo, Roger Guimera, Matthias Blueher, Jose Ignacio Martin-Subero, Pablo M. Garcia-Roves
Summary: This study systematically assessed metabolic plasticity in diet-induced obese mice after a combined nutritional and exercise intervention, and found that there is a significant metabolic dysfunction in visceral white adipose tissue, which leads to a breakdown of metabolic plasticity.
Article
Cell & Tissue Engineering
Xiaotong Hong, Joan Isern, Silvia Campanario, Eusebio Perdiguero, Ignacio Ramirez-Pardo, Jessica Segales, Pablo Hernansanz-Agustin, Andrea Curtabbi, Oleg Deryagin, Angela Pollan, Jose A. Gonzalez-Reyes, Jose M. Villalba, Marco Sandri, Antonio L. Serrano, Jose A. Enriquez, Pura Munoz-Canoves
Summary: Mitochondrial dynamics play a crucial role in the regenerative capacity of satellite cells. Aging or genetic impairment disrupts mitochondrial fission in satellite cells, leading to metabolic dysfunction and increased oxidative stress, resulting in muscle regenerative failure. Restoring mitochondrial dynamics can rescue impaired satellite cell function. These findings have implications for regeneration therapies in muscle aging.
Article
Biochemistry & Molecular Biology
Pablo Hernansanz-Agustin, Jose Antonio Enriquez
Summary: Oxidative phosphorylation is a common process in which an electrochemical gradient is generated across the inner mitochondrial membrane to provide energy to the cell. Some organisms maintain non-energy conserving oxidases as a bypass to oxidative phosphorylation. In recent years, the use of alternative oxidases has contributed to the understanding of various processes in bioenergetics and mitochondria.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Immunology
Stefanie K. Wculek, Ignacio Heras-Murillo, Annalaura Mastrangelo, Diego Mananes, Miguel Galan, Veronica Miguel, Andrea Curtabbi, Coral Barbas, Navdeep S. Chandel, Jose Antonio Enriquez, Santiago Lamas, David Sancho
Summary: This study identifies oxidative phosphorylation (OXPHOS) as the most important process in distinguishing tissue macrophage populations in different organs. Impairing OXPHOS has differential effects on tissue macrophages, leading to reduced alveolar macrophages and selectively depleting pro-inflammatory white adipose tissue macrophages. OXPHOS is critical for maintaining tissue macrophages with high lipid-handling activity, making it a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Raquel Moreno-Loshuertos, Nieves Movilla, Joaquin Marco-Brualla, Ruth Soler-Agesta, Patricia Ferreira, Jose Antonio Enriquez, Patricio Fernandez-Silva
Summary: Mitochondrial ATP synthase plays a crucial role in ATP production and is associated with various human diseases. A mouse cell line with the first mt-Atp6 pathological mutation was generated and characterized, showing similarities to human diseases caused by MT-ATP6 mutations. The mutant cells exhibited impaired ATP synthesis, defective OXPHOS activity, and altered mitochondrial function. Additionally, they displayed reduced tumorigenic potential, suggesting a potential protective role of ATP synthase inhibition in certain cancer types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tasnim Arroum, Marie-Theres Borowski, Nico Marx, Frank Schmelter, Martin Scholz, Olympia Ekaterini Psathaki, Michael Hippler, Jose Antonio Enriquez, Karin B. Busch
Summary: The mitochondrial respiratory or electron transport chain (ETC) and ATP synthase collaborate in the process of oxidative phosphorylation (OXPHOS) to convert reduction power into ATP. Supramolecular assemblies known as respiratory supercomplexes (SC) are formed by three out of the four ETC complexes to adapt to changing conditions. NDUFB10, a subunit of complex I, plays a role in complex I and supercomplex assembly as well as bioenergetics function. NDUFB10 knockout results in decreased SCAF1, reduced respiration, and mitochondrial membrane potential, potentially due to loss of proton pumping.
BIOLOGICAL CHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Anna S. S. Monzel, Jose Antonio Enriquez, Martin Picard
Summary: The authors argue that the terms 'mitochondrial function' and 'mitochondrial dysfunction' are not sufficient to describe the diverse features and behaviors of mitochondria within cells. They propose a hierarchical terminology system to improve specificity in mitochondrial science, which will enhance our understanding of the contribution of mitochondria to cellular and organismal health and promote synergy with other disciplines.
Article
Medicine, General & Internal
Rebekah Jaung, Lynne Soon-Chean Park, Joohyun Justine Park, David Tokiharu Mayeda, Changzoo Song
Summary: This study examines the experiences of racism among New Zealand Asians and its impact on their life satisfaction during the COVID-19 pandemic.
NEW ZEALAND MEDICAL JOURNAL
(2022)
Article
Cell Biology
Morena Scotece, Carlos Vaamonde-Garcia, Ana Victoria Lechuga-Vieco, Alberto Centeno Cortes, Maria Concepcion Jimenez Gomez, Purificacion Filgueira-Fernandez, Ignacio Rego-Perez, Jose Antonio Enriquez, Francisco J. Blanco
Summary: This study reveals that mtDNA variations have an impact on joint damage during the aging process. By using a conplastic mouse model, it is shown that genetic manipulation of mtDNA can ameliorate joint aging damage, indicating that mtDNA variability is a prognostic factor for aging-related osteoarthritis (OA).
Meeting Abstract
Biochemistry & Molecular Biology
B. Cicuendez, C. Folgueira, A. Mora, J. A. Lopez, A. Curtabbi, P. Hernansanz-Agustin, R. Romero-Becerra, E. Rodriguez, L. Leiva-Vega, J. Vazquez, J. A. Enriquez, M. Rincon, G. Sabio
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)