4.7 Article

Mechanisms of change in gel water-holding capacity of myofibrillar proteins affected by lipid oxidation: The role of protein unfolding and cross-linking

期刊

FOOD CHEMISTRY
卷 344, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128587

关键词

Linoleic acid; Myofibrillar proteins; Oxidation; Gel WHC

资金

  1. National Natural Science Foundation of China [31672605]
  2. Natural Science Foundation of Chongqing, China [cstc2018jcyjAX0242]
  3. Chongqing Graduate Research and Innovation Project [CYS19115]

向作者/读者索取更多资源

The study found that moderate concentrations of linoleic acid can increase the water-holding capacity of beef myofibrillar protein gels, but high concentrations of linoleic acid can decrease the water-holding capacity.
This work explored the effects of protein unfolding and cross-linking induced by lipid oxidation (linoleic acid, OLA) on the gel water-holding capacity (WHC) of beef myofibrillar proteins (MP). Medium concentration of OLA (<= 6 mM) caused the increase of gel WHC from 55.2% to 65.1%, while relative high OLA concentration (>6 mM) decreased the gel WHC. When the OLA concentrations increased from 0 to 10 mM, the population of immobile water of gel decreased from 92.91% to 78.97%, whereas that of free water increased from 6.13% to 19.80%, suggesting that OLA treatment regardless concentration was harmful for gel WHC. However, medium OLA concentrations (<= 6 mM) caused the shifting of a-helixes to beta-sheets in MP gel, exerting positive effect on gel WHC. Protein unfolding and cross-linking jointly determined the increased gel WHC at moderate oxidative modification. Additionally, the protein aggregation at high OLA concentration resulted in decreased gel WHC.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Food Science & Technology

An underlying softening mechanism in pale, soft and exudative - Like rabbit meat: The role of reactive oxygen species - Generating systems

Zhaoming Wang, Hui Zhou, Kai Zhou, Juncai Tu, Baocai Xu

Summary: This study found that during aging of rabbit meat, PSE-like meat samples showed significantly higher values in peroxides, protein oxidation and degradation compared to normal meat samples, indicating that the ROS-generating system played a potential role in the softening process of PSE-like meat.

FOOD RESEARCH INTERNATIONAL (2022)

Article Food Science & Technology

Inhibition of phenolics on the in vitro digestion of noodles from the view of phenolics release

Ruibin Wang, Ming Li, Gang Wu, Xiaodan Hui, Juncai Tu, Margaret A. Brennan, Boli Guo, Charles S. Brennan

Summary: The inhibitory mechanism of buckwheat phenolics on starch digestion was studied in extruded noodles, showing a dose-effect relationship between reducing sugar released and the release of phenolics. Phenolics bind with alpha-amylase through hydrogen bonds and hydrophobic interaction, inhibiting its activity and reducing starch digestion. The release of phenolics during cooking and digestion explains the reduction in starch digestion.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2022)

Article Food Science & Technology

Utilisation of dried shiitake, black ear and silver ear mushrooms into sorghum biscuits manipulates the predictive glycaemic response in relation to variations in biscuit physical characteristics

Juncai Tu, Margaret Anne Brennan, Xiaodan Hui, Ruibin Wang, Donatella Peressini, Weidong Bai, Ping Cheng, Charles Stephen Brennan

Summary: The study found that incorporating mushroom powders into sorghum flour can increase water absorption capacity and swelling power, decrease pasting viscosities, reduce biscuit diameter, thickness, and weight loss with increased mushroom powder addition, and significantly affect the color parameters of biscuits. Enrichment with shiitake and black ear mushroom can increase the hardness of biscuits, and reduce the predicted glycaemic response of sorghum biscuits.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2022)

Article Food Science & Technology

Umami and umami-enhancing peptides from myofibrillar protein hydrolysates in low-sodium dry-cured Spanish mackerel (Scomberomorus niphonius) under the action of Lactobacillus plantarum

Weidong Bai, Jinxin Liang, Wenhong Zhao, Min Qian, Xiaofang Zeng, Juncai Tu, Juan Yang

Summary: Lactobacillus plantarum is involved in the production of low-salt dry-cured Spanish mackerel and affects the degradation of myofibrillar protein and production of umami peptides. The study identified five umami-enhancing peptides and showed the potential of Lactobacillus plantarum to improve the hydrolysis of myofibrillar protein and the production of umami peptides in dry-cured mackerel.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2022)

Article Food Science & Technology

The structural rearrangement and depolymerisation induced by high-pressure homogenisation inhibit the thermal aggregation of myofibrillar protein

Chang Su, Zhifei He, Zefu Wang, Dong Zhang, Hongjun Li

Summary: This study investigated the effects of heating on the dissolution and aggregation of high-pressure homogenisation (HPH)-treated myofibrillar protein (MP) in a low ionic strength medium. The results showed that HPH treatment increased the solubility and decreased the turbidity of MP during heating. Rheological measurements indicated that HPH treatment reduced the thermal aggregation ability of MP, except at around 50 degrees C. Proteomic analysis suggested that HPH treatment disrupted cytoskeletal proteins and promoted structural rearrangement of MP, leading to the formation of oligomers with stronger steric hindrance and electrostatic repulsion, thus inhibiting thermal-induced aggregation. Depolymerisation induced by HPH treatment also hindered the assembly of myosin, preventing the formation of strand-type aggregates during heating. In vitro digestion experiments showed that HPH treatment improved the digestibility of MP, regardless of heat treatment.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2022)

Article Food Science & Technology

Effects of lysine and arginine addition combined with high-pressure microfluidization treatment on the structure, solubility, and stability of pork myofibrillar proteins

Dong Zhang, Zhicheng Wu, Jinggang Ruan, Yizhi Wang, Xueyi Li, Min Xu, Jie Zhao, Hongbin Lin, Ping Liu, Zhaoming Wang, Hongjun Li

Summary: This study investigates the effects of lysine and arginine addition combined with high-pressure microfluidization treatment on the structure, solubility and stability of pork myofibrillar proteins. The results show that these treatments significantly increase the solubility and stability of the proteins, and also result in the production of soluble polymers.

LWT-FOOD SCIENCE AND TECHNOLOGY (2022)

Article Food Science & Technology

Combination of millet pepper and garlic water extracts improves the antioxidant capability of myofibrillar protein under malondialdehyde-induced oxidative modification

Dong Zhang, Jiamin Liu, Jinggang Ruan, Zhenju Jiang, Fanyi Gong, Wenjing Lei, Xinyi Wang, Jie Zhao, Qingyang Meng, Min Xu, Jie Tang, Hongjun Li

Summary: This study aimed to investigate the effect of millet pepper water extract (PWE) and garlic water extract (GWE) on the antioxidant capability of myofibrillar protein (MP) under malondialdehyde-induced oxidation. Mass spectrum analysis showed that the main antioxidant substances in PWE are dihydrocapsaicin, L-tryptophan, and 5-aminolevulinic acid, and in GWE are L-tryptophan, 5-aminolevulinic acid, and uridine. The addition of PWE and GWE inhibits the formation of carbonyl compounds and cross-linked polymers in MP, and inhibits the reduction in MP solubility, fluorescence spectral intensity, and UV spectral intensity. PWE increases the antioxidant capability of MP better than GWE due to its higher content of main antioxidant components. The differences in main antioxidant components between PWE and GWE provide them with a synergistic effect on enhancing the antioxidant capability of MP.

LWT-FOOD SCIENCE AND TECHNOLOGY (2023)

Article Chemistry, Applied

Acidic polysaccharides from black ear and silver ear mushrooms modulated the release and transport of glucose from gelatinised sorghum starch during digestion

Juncai Tu, Benu Adhikari, Margaret Anne Brennan, Siyuan Luo, Ping Cheng, Weidong Bai, Charles Stephen Brennan

Summary: This study found that adding 0.6% acidic polysaccharides from Auricularia auricula-judae and Tremella fuciformis mushrooms to gelatinised sorghum starch increased the proportion of high molecular weight a-dextrin and delayed glucose diffusion. The transportation of glucose was reduced by 34.2% and 38.7% when sorghum starch was gelatinised with AAP and TFP, respectively. This reduction was due to the inhibition of α-glucosidase by AAP/TFP and the difficulty in hydrolyzing high molecular weight maltooligosaccharides. Additionally, AAP/TFP could bond to the glucose transporter (SGLT1) and impede glucose transport.

FOOD CHEMISTRY (2023)

Article Chemistry, Applied

Interactions between sorghum starch and mushroom polysaccharides and their effects on starch gelatinization and digestion

Tu Juncai, Benu Adhikari, Margaret Anne Brennan, Cheng Ping, Bai Weidong, Charles Stephen Brennan

Summary: This study aimed to investigate the interactions between sorghum starch and mushroom polysaccharides (MPs) obtained from different cultivars, and their effects on gelatinization, retrogradation, digestibility, and microstructures of starch. The results showed that MPs could reduce the degree of gelatinization, retrogradation energy, and digestibility of sorghum starch, and increase the particle size of digesta in a concentration dependent manner. Confocal laser scanning microscopic images revealed that LEP could coat sorghum starch granules through electrostatic interaction, promoting starch retrogradation. AAP and TFP were able to suppress starch crystallization in sorghum starch gels through hydrophobic interaction with starch. The interaction between sorghum starch and MPs depended on the nature of MPs and their concentration, providing fundamentals for the utilization of MPs as glycaemic controller of starch and starch-rich foods.

FOOD HYDROCOLLOIDS (2023)

Article Food Science & Technology

Effect of partial NaCl replacement by KCl and calcium ascorbate on protein and lipid oxidation in bacon during storage

Jinggang Ruan, Zhicheng Wu, Xiangyan Kong, Yuqi He, Lanqin Shu, Nanxi Huang, Jiaxin Chen, Jie Tang, Dong Zhang, Hongjun Li

Summary: This study aimed to compare the protein and lipid oxidation of bacon with alternative salt (KCl and calcium ascorbate) and traditional bacon (NaCl) during a two-month storage. The alternative salt bacon had higher carbonyl content and TBARS, but lower sulphhydryl content, fluorescence intensity, and alpha-helix structure compared to the traditional bacon. As storage duration increased, both types of bacon showed increased oxidation, with the alternative salt bacon exhibiting stronger oxidation. These findings suggest that alternative salts can induce physicochemical changes in bacon. Further investigation is needed to understand the formation of volatile flavor and taste compounds during storage.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2023)

Article Agriculture, Multidisciplinary

Novel Umami-, Salty-, and Kokumi-Enhancing γ-Glutamyl Tripeptides Synthesized with the Bitter Dipeptides from Defatted Peanut Meal Protein Hydrolysate

Juncai Tu, Jing Guo, Hao Dong, Ping Cheng, Charles Brennan, Weidong Bai, Xiaofang Zeng, Juan Yang

Summary: Gamma-glutamylation by Bacillus amyloliquefaciens L-glutaminase was used to reduce the bitterness of defatted peanut meal protein hydrolysates (DPMHs) and generate gamma-glutamylated products (DPMHG). The results showed that gamma-glutamylation decreased the bitterness and enhanced the taste attributes of the products, especially for DPMH-G-Q. This study provides a new method to improve the taste of protein hydrolysates by synthesizing gamma-glutamyl tripeptides.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2023)

Article Food Science & Technology

Effect of partial replacement of NaCl by KCl and calcium ascorbate on the dynamics of bacterial community during storage of bacon based on 16S rRNA gene amplicon sequencing

Jinggang Ruan, Jingbing Xu, Zhicheng Wu, Yong Tang, Dan Xiang, Xue Li, Yiru Yu, Xinrui Xie, Jie Tang, Dong Zhang, Hongjun Li

Summary: The study used high-throughput sequencing to track changes in abundance and composition of microbial communities in low-sodium bacon during storage. The use of potassium chloride and calcium ascorbate as substitutes for sodium chloride resulted in reduced microbial population size and altered community structure. The most prevalent bacterial groups identified at the genus level were Ralstonia, Bacillus, Sphingomonas, and Lactobacillus. Correlation analysis revealed a significant relationship between physicochemical indicators and the bacterial community. Using sodium chloride as an alternative promoted the growth of Lactobacillus, which had variable effects on the bacterial community structure. The strong correlation between overall sensory score and microorganisms suggests that partial replacement of sodium chloride in bacon can impact its quality.

LWT-FOOD SCIENCE AND TECHNOLOGY (2023)

Article Food Science & Technology

Changes in Texture Characteristics and Special Requirements of Sichuan-Style Braised Beef for Industrial Production: Based on the Changes in Protein and Lipid of Beef

Xiaoli Pu, Jinggang Ruan, Zhicheng Wu, Yong Tang, Ping Liu, Dong Zhang, Hongjun Li

Summary: This study aimed to determine the optimal stewing time for industrialized preparation of Sichuan-style braised beef with different demands. The study found that the texture properties of braised beef were affected by moisture content, oxidation of proteins and lipids, and integrity of the muscle fibers during the stewing process. Based on texture traits, a stewing time of 60 minutes was recommended for industrialized production processes when braised beef is cooked with other ingredients, while stewing times of 120 or 150 minutes were recommended for industrialized production processes when consumers only use simple heating to eat the product.
Article Chemistry, Applied

Probing the mechanism of interaction between capsaicin and myofibrillar proteins through multispectral, molecular docking, and molecular dynamics simulation methods

Zhicheng Wu, Jingbing Xu, Jinggang Ruan, Jiaxin Chen, Xue Li, Yiru Yu, Xinrui Xie, Jie Tang, Dong Zhang, Hongjun Li

Summary: The interaction between myofibrillar proteins (MPs) and capsaicin (CAP) was investigated using multispectral, molecular docking, and molecular dynamics simulation methods. The study found that the resulting complex increased the hydrophobicity of the tryptophan and tyrosine microenvironment as revealed by fluorescence spectral analysis. It was also discovered that the interaction between CAP and MPs was primarily facilitated by hydrogen bonding, van der Waals forces, and hydrophobic interactions, as suggested by molecular docking models and molecular dynamics simulations.

FOOD CHEMISTRY-X (2023)

Article Food Science & Technology

Formation mechanism of texture characteristics of pork belly during stewing: focusing on the effects of myofibrillar protein modification

Zhicheng Wu, Jiamin Liu, Jinggang Ruan, Min Xu, Zhaoming Wang, Dong Zhang

Summary: The effects of stewing time and the presence of fat on the physicochemical characteristics of meat were investigated. The results showed that prolonged stewing time and the presence of fat promoted the oxidation degradation of protein in meat, leading to changes in texture.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2023)

Article Chemistry, Applied

The first harmonised total diet study in Portugal: Vitamin D occurrence and intake assessment

M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira

Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

The digestion fates of lipids with different unsaturated levels in people with different age groups

Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu

Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Fabrication and characterization of chitosan-pectin emulsion-filled hydrogel prepared by cold-set gelation to improve bioaccessibility of lipophilic bioactive compounds

Hyunjong Yu, Huisu Kim, Pahn-Shick Chang

Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

The effect of lactic acid bacteria fermentation on physicochemical properties of starch from fermented proso millet flour

Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke

Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Novel competitive electrochemical impedance biosensor for the ultrasensitive detection of umami substances based on Pd/Cu-TCPP(Fe)

Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu

Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Identification and comparison of milk fat globule membrane and whey proteins from Selle Français, Welsh pony, and Tieling Draft horse mare's milk

Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang

Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Phenols and saliva effect on virgin olive oil aroma release: A chemical and sensory approach

Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales

Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Preparation and properties of pH-sensitive cationic starch nanoparticles

Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen

Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Direct seeding compromised the vitamin C content of baby vegetables and the glucosinolate content of mature vegetables in Asian leafy brassicas

Andrea Koo, Vinayak Ghate, Weibiao Zhou

Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

ACE inhibitory peptides from enzymatic hydrolysate of fermented black sesame seed: Random forest-based optimization, screening, and molecular docking analysis

Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie

Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Exploration of digestion-resistant immunodominant epitopes in shrimp (Penaeus vannamei) allergens

Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun

Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Effect of milling on in vitro Digestion-Induced release and bioaccessibility of active compounds in rice

Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan

Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Unraveling proteome changes of Sunit lamb meat in different feeding regimes and its relationship to flavor analyzed by TMT-labeled quantitative proteomic

Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin

Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Mechanism of aroma enhancement methods in accelerating Congou black tea acidification subjected to room temperature storage

Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni

Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Immobilizing amyloglucosidase on inorganic hybrid nanoflowers to prepare time-temperature integrators for chilled pork quality monitoring

Lin Wang, Falai Ma, Zihan Li, Yan Zhang

Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.

FOOD CHEMISTRY (2024)