Salinity alleviates the toxicity level of ozone in a halophyte Mesembryanthemum crystallinum L

Mesembryanthemum crystallinum (Ice plant) is an annual halophytic plant species spread in the coastal areas of the Mediterranean Sea, Egypt. Information about the behaviour of halophytes under the future concentration of ozone (O-3) is scanty. Therefore, we have assessed the effects of elevated O-3 (ambient + 20 ppb), moderate salinity (200 mM NaCl), and their combined treatment (salinity + elevated O-3) on various morphological, growth, physiological, biochemical and anatomical parameters of Egyptian ice plant. Under salinity stress, plant growth, percentage of pigmented leaf and its thickness, ROS levels, antioxidative enzymes, and ROS scavenging activities were increased, while photosynthetic pigments and efficiency were decreased compared to the control. Elevated O-3 exposure led to reductions in most of the growth parameters and pigments, while ROS levels, histochemical localization of H2O2 and center dot O-2(-), antioxidative enzymes and nonenzymatic antioxidants (betacyanin, phenolics, thiols and ascorbic acid) showed increases. Surprisingly, salinity alleviated the oxidative stress of elevated O-3 due to the rise of SOD activity, antioxidant compounds, and a decrease of center dot O-2(-) production rate with concomitant increases of most of the growth parameters. Thick lower collenchyma and enhancement of xylem parenchyma under O-3 and combined treatment suggested that anatomical acclimation also operated under O-3 stress and salinity played a vital role in the growth of this plant under combined stress. Results showed that salt is essential for the optimum development of this species and its role is extended to alleviate the oxidative damage caused by elevated O-3. The results further recommend the use of Egyptian M. crystallinum as a O-3 tolerant crop for saline areas along the Mediterranean Sea coast. [GRAPHICS] .

Automated summary

Ice plant is a halophytic plant species, and little is known about its response to future ozone concentration. This study evaluated the effects of elevated O3 concentration, salinity, and their combination on various parameters of ice plant. The results showed that salinity played a crucial role in alleviating oxidative stress caused by elevated O3, emphasizing the importance of salt for the growth of this plant under combined stress.