Article
Horticulture
Chuanhong Liu, Gengxing Song, Yonghui Zhao, Bing Fang, Zhiyong Liu, Jie Ren, Hui Feng
Summary: This study improves the embryogenesis and reduces the embryonic mortality in cut flower ornamental kale through histone deacetylation inhibitor treatment, with optimal TSA concentrations determined by variety, leading to a higher proportion of double haploid regenerated plants.
Article
Horticulture
Jiaqi Zou, Xiao Zou, Zhichao Gong, Gengxing Song, Jie Ren, Hui Feng
Summary: Curly kale, known for its rich nutrition and health benefits, is highly sought after and considered one of the healthiest vegetables. However, conventional breeding methods for this vegetable take a long time to produce homozygous inbred lines. In this study, the isolated microspore culture (IMC) technique was successfully used to accelerate the breeding cycle of curly kale through efficient cytokinin thidiazuron (TDZ) treatment. The results showed that 0.2 mg/L TDZ had the optimal effect on embryo survival and plant regeneration, significantly shortening the breeding cycle.
Article
Horticulture
Ying Zhao, Wenfeng Zheng, Jie Li, Xinghua Qi, Hui Feng, Yun Zhang
Summary: The study successfully established an effective microspore culture protocol for broccoli, which improved the frequency of microspore embryogenesis and plantlet regeneration.
SCIENTIA HORTICULTURAE
(2022)
Article
Food Science & Technology
Jacinta Collado-Gonzalez, Maria Carmen Pinero, Gines Otalora, Josefa Lopez-Marin, Francisco M. del Amor
Summary: The study investigated the effects of exogenous arginine application on cauliflower florets under heat stress, showing that the combination of high temperature and arginine increased antioxidant activity and nutrient content.
Article
Multidisciplinary Sciences
K. N. Rakshita, Shrawan Singh, Veerendra Kumar Verma, Brij Bihari Sharma, Navinder Saini, Mir Asif Iquebal, Akanksha Sharma, Shyam Sunder Dey, T. K. Behera
Summary: The study analyzed the molecular and agro-morphological diversity in 92 diverse cauliflower genotypes, along with cabbage and broccoli genotypes. Differences in traits and genetic diversity were observed, indicating potential for genetic improvement and heterosis breeding in cauliflower.
Article
Plant Sciences
Mingzhao Zhu, Limei Yang, Yangyong Zhang, Mu Zhuang, Jialei Ji, Xilin Hou, Zhansheng Li, Fengqing Han, Zhiyuan Fang, Honghao Lv, Yong Wang
Summary: In this study, clubroot resistance (CR) genes from Brassica rapa were introduced into the cabbage genome, making it resistant to clubroot disease. The mechanism of gene introgression was explored through cytoplasmic replacement and distant hybridization, revealing homoeologous exchange as the basis for the introgression of CR resistance. The successful introduction of CR into the cabbage genome provides valuable insights for creating introgression lines in other species of interest.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Chuanhong Liu, Gengxing Song, Bing Fang, Zhiyong Liu, Jiaqi Zou, Shiyao Dong, Sai Du, Jie Ren, Hui Feng
Summary: Isolated Microspore Culture (IMC) is an efficient method for obtaining homozygous strains, but it is challenging in ornamental kale. Histone acetylation, an important epigenetic mechanism, can promote microspore embryogenesis. The study found that the histone deacetylase inhibitor SAHA can enhance embryogenesis in microspores of cut-flower ornamental kale.
Article
Plant Sciences
Daniela Losacco, Marina Tumolo, Pietro Cotugno, Natalia Leone, Carmine Massarelli, Stefano Convertini, Angelo Tursi, Vito Felice Uricchio, Valeria Ancona
Summary: Biochar application can reduce nitrate pollution and increase crop yield. This experiment showed that biochar affected soil properties and nitrogen leachate, and increased the marketable yield of horticultural crops.
Article
Plant Sciences
Andreea Lupitu, Cristian Moisa, Flavia Bortes, Denisa Peteleu, Mihaela Dochia, Dorina Chambre, Virgiliu Ciutina, Dana Maria Copolovici, Lucian Copolovici
Summary: This study explores the interplay between elevated atmospheric CO2, drought stress, and plant physiological responses. The findings suggest that elevated CO2 accelerates assimilation rate decline during drought and influences photosynthetic components and electron transport. Additionally, drought-induced monoterpene emissions, polyphenols, flavonoid concentrations, and chlorophyll responses were affected by CO2 concentration and species-specific responses. These findings contribute to a nuanced understanding of CO2-drought interactions and their intricate effects on plant physiology.
Article
Plant Sciences
Simone Treccarichi, Hajer Ben Ammar, Marwen Amari, Riccardo Cali, Alessandro Tribulato, Ferdinando Branca
Summary: Marker assisted selection can be used to identify lost alleles in Brassica oleracea crops and improve their resistance and product quality. Analysis of genomic correlations and SSR loci variation can help find genomic regions involved in the hypertrophy of inflorescences.
Article
Biotechnology & Applied Microbiology
Weishu Chen, Yun Zhang, Shengnan Huang, Jie Ren, Hui Feng
Summary: Isolated microspore culture (IMC) is a potential alternative technique in plant breeding for producing homozygous lines. However, low embryogenesis rate, high embryo death rate, and low chromosome doubling frequency limit its implementation. This study investigated the effects of L-ascorbic acid sodium salt (VcNa) concentrations on embryogenesis and regeneration in kale using IMC, and developed an artificial chromosome doubling protocol for kale microspore-derived haploids. The results showed that 4 μM VcNa at pH 5.84 was the optimum concentration for embryogenesis, and dipping haploid plantlet roots in colchicine solution at specific concentrations and durations achieved nearly 50% chromosome doubling rate. This study provides an effective tissue culture process for promoting chromosomal ploidy of microspore-derived regenerated plants.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Congcong Kong, Henan Su, Siping Deng, Jialei Ji, Yong Wang, Yangyong Zhang, Limei Yang, Zhiyuan Fang, Honghao Lv
Summary: In this study, the regulatory roles of DNA methylation and miRNA in early microspore embryogenesis (ME) were investigated in cabbage. The results showed that heat shock induced DNA methylation and miRNA, which influenced ME by modulating reactive oxygen species (ROS) response and abscisic acid (ABA) signaling. These findings provide new insights into the molecular regulation of ME induced by heat shock.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Ranjan K. Shaw, Yusen Shen, Zhenqing Zhao, Xiaoguang Sheng, Jiansheng Wang, Huifang Yu, Honghui Gu
Summary: Cauliflower is an important vegetable crop globally, but its production is constrained by destructive fungal diseases like downy mildew. Developing resistant varieties through molecular breeding is crucial for sustainable cauliflower production. Identifying novel resistant resources and mapping resistance QTLs are essential for developing durable resistant cultivars.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Horticulture
Jacinta Collado-Gonzalez, Maria Carmen Pinero, Gines Otalora, Josefa Lopez-Marin, Francisco M. del Amor
Summary: The combination of heat stress and exogenous spermidine led to an increase in antioxidant activity, total phenolic compounds, sugars, proteins, and mineral components in cauliflower florets. Foliar application of spermidine at high temperature increased the contents of inositol, fructose, and sucrose, while also resulting in the accumulation of sulphate, phosphate, and nitrate.
SCIENTIA HORTICULTURAE
(2021)
Article
Thermodynamics
Hande Mutlu Ozturk
Summary: This study investigates the energy and exergy analyses of a vacuum cooling system for cooked foods, with a focus on boiled cauliflower as a case study. The study analyzes the effects of pressure drop, relative humidity, and average temperature on exergy product and total exergy, as well as the parameters affecting exergetic efficiency and COP for the vacuum cooling system. Experimental results show that the COP of vacuum cooling can reach 26 and exergy efficiency can increase to 91% when pressure is close to absolute zero.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)