Article
Biochemistry & Molecular Biology
Qiang Chen, Kun Cui, Zengqi Zhao, Xiang Xu, Yongtao Liu, Yanan Shen, Fan Chen, Kangsen Mai, Qinghui Ai
Summary: In this study, we revealed the mechanism by which LPS stabilizes HIF-1α by increasing its acetylation via the PARP1-SIRT1 and ACLY-Tip60 pathways, and found that reducing HIF-1α acetylation could alleviate LPS-induced inflammation.
Review
Medicine, General & Internal
Andreas Zimmermann, Frank Madeo, Abhinav Diwan, Junichi Sadoshima, Simon Sedej, Guido Kroemer, Mahmoud Abdellatif
Summary: Mitochondrial dysfunction and its removal through mitophagy play a central role in ageing and chronic diseases. The regulation of mitophagy involves metabolic pathways and various molecular switches. Understanding the intersection of metabolic regulation and mitophagy can provide insights for delaying ageing and treating mitochondria-associated chronic diseases.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Rana M. Nassif, Elias Chalhoub, Pia Chedid, Margarita Hurtado-Nedelec, Elia Raya, Pham My-Chan Dang, Jean-Claude Marie, Jamel El-Benna
Summary: Metformin exhibits anti-inflammatory effects by inhibiting the differentiation of human monocytes into proinflammatory macrophages and by limiting ROS production by macrophages via the activation of AMPK.
Review
Nutrition & Dietetics
Jyothi Dhuguru, Ryan W. W. Dellinger, Marie E. E. Migaud
Summary: Dietary vitamin B3 components have been found to increase NAD(+) levels, which decline with age and disease. Supplementation with NAD(+) biosynthetic intermediates, such as NR and NMN, can effectively raise NAD(+) levels. In addition, inhibiting NAD(+)-consuming enzymes or activating biosynthetic pathways is also a viable approach to increase NAD(+) and NADH. However, the pharmacological significance of NAD(H) catabolites is often overlooked, despite some of these metabolites serving as biomarkers in physiological disorders.
Article
Chemistry, Applied
Jingyu Wang, Elif Erdem, John M. Woodley
Summary: Biocatalytic oxidation holds promise for the selective synthesis of active pharmaceutical intermediates. Bubbling air or oxygen is an effective method to enhance oxygen transfer, but it leads to enzyme deactivation. This study investigates the stability of NAD-(P)H oxidase (NOX) at different gas-liquid interfaces and provides insights for improving biocatalyst performance.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Toxicology
Yewen Cong, Yujing Hong, Dandan Wang, Pei Cheng, Zhisheng Wang, Changming Xing, Wenxing Sun, Guangfei Xu
Summary: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is widely recognized as the most toxic and prevalent carcinogen in the world. Exposure to TCDD causes disorder in liver lipid metabolism and leads to steatosis. The molecular mechanism of TCDD-induced liver lipid accumulation is not fully understood.
TOXICOLOGICAL SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Andreas S. Bommarius
Summary: While many oxidases are active and selective, they often lack stability for chemical synthesis or large-scale processing due to various stresses, such as loss of flavin co-enzyme, overoxidation in the presence of reactive oxygen species, or instability in the presence of air/water interfaces if the co-substrate oxygen is supplied by sparging.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Isabella D. Cooper, Yvoni Kyriakidou, Lucy Petagine, Kurtis Edwards, Bradley T. Elliott
Summary: The pursuit of longevity and healthspan requires overcoming chronic diseases of aging such as cardiovascular disease, hypertension, cancer, dementias, and type 2 diabetes. Hyperinsulinemia and oxidative stress play a crucial role in the development of these diseases, and fasting and ketogenic diets can improve antioxidant synthesis and mitochondrial function, leading to better health.
Review
Pharmacology & Pharmacy
Gulam Mohmad Rather, Alvinsyah Adhityo Pramono, Zoltan Szekely, Joseph R. Bertino, Philip Michael Tedeschi
Summary: Cancer cells require elevated levels of NADPH for nucleotide synthesis and protection from ROS, which can be generated in several ways. Targeting NADPH synthesis may provide an opportunity for cancer treatment.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Chemistry, Physical
Jingyu Wang, John M. Woodley
Summary: This paper investigates the regeneration of important cofactors NAD(P)H and NAD(P) in biocatalytic synthesis for ensuring the economic sustainability of the process. The kinetic stability of a water-forming NAD(P)H oxidase (NOX) for in-situ cofactor regeneration in a bubble column is studied. The results show that NOX exhibits two-stage and single-stage deactivation kinetics, with aggregation and unfolding at the gas-liquid interface being the main cause of deactivation.
Article
Chemistry, Multidisciplinary
Sooyoung Lim, Hee-wang Yoo, Sharad Sarak, Byung-gee Kim, Hyungdon Yun
Summary: The study optimized the enzymatic production of dodecanedioic acid from fatty acids, identified a suitable enzyme for the process, and achieved improved productivity through fusion protein method.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Neurosciences
Yang Zhang, Xun Guo, Zhifeng Peng, Chang Liu, Lili Ren, Jia Liang, Peng Wang
Summary: This study investigates the potential role of nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1) in blood-brain barrier (BBB) damage after ischemic stroke. The results suggest that recombinant human NMNAT1 (rh-NMNAT1) can reduce infarct volume, improve functional outcome, and decrease BBB permeability. The protective effects of rh-NMNAT1 may be mediated through the regulation of nicotinamide adenine dinucleotide (NAD(+))/NADH ratio and Sirtuin 1 (SIRT1) level. Rh-NMNAT1 treatment can also decrease the levels of acetylated nuclear factor-kappa B, acetylated p53, and matrix metalloproteinase-9 in ischemic microvessels. The findings indicate that NMNAT1 may be a potential therapeutic target for reducing BBB disruption after ischemic stroke.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Chemistry, Applied
Marc Biermann, Martin Schuermann, Thomas Schmitges, Andreas Vogel, Jan Brummund
Summary: A biotransformation method to produce (R)-Undecavertol has been developed and successfully demonstrated at the pilot scale with high productivity. By using a highly selective alcohol dehydrogenase and NAD(P)H oxidase, racemic resolution of the substrate is achieved, and oxygen limitation is prevented by the supply of pure oxygen.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Article
Chemistry, Multidisciplinary
Maodi Wang, Huicong Dai, Qihua Yang
Summary: In this study, a metal nanoparticle and metal complex integrated core-shell nanoreactor was prepared for H2-driven NAD(P)H regeneration. The integrated catalyst showed enhanced activity and selectivity in NAD(P)H regeneration due to the spillover effect of activated H species. The size-sieving effect of the porous organic polymer enabled the coupling of the core-shell nanoreactor with aldehyde ketone reductase for chemoenzymatic reduction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Plant Sciences
Ian Max Moller, Allan G. Rasmusson, Olivier Van Aken
Summary: The study of plant mitochondria began in the 1950s, focusing on establishing basic properties and unique characteristics compared to mammalian mitochondria. With advancements in molecular biology and genomics, further unique features were discovered, including the large size of plant mtDNA, unique mRNA maturation process, and a large proteome with many unique proteins.