期刊
CHEMICAL ENGINEERING JOURNAL
卷 424, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.130348
关键词
Peptide; Covalent self-assembly; Dark-field hyperspectral imaging; Cell tracking; Turbatrix aceti nematodes
资金
- National Natural Science Foundation of China [22025207, 21977095]
- Chinese Postdoctoral Science Foundation [2020M680674]
- Russian Fund for Basic Research [18-53-80067 BRICS_t]
- National Natural Science Fund BRICS STI Framework Program [51861145304]
- Key Research Program of Frontier Sciences of Chinese Academy of Sciences (CAS) [QYZDB-SSW-JSC034]
Peptide nanoparticles fabricated through covalent self-assembly of dipeptides and genipin enhance optical scattering intensity and stability, allowing for easy visualization and tracking in cells and organisms for Enhanced dark-field hyperspectral imaging (EDF-HSI). These results provide a promising approach for visualization and analysis of biomaterial activities in vitro and in vivo.
Enhanced dark-field hyperspectral imaging (EDF-HSI) integrates advanced spectroscopy and imaging techniques, enabling fast and quantitative insights into the interactions between nanomaterials and organisms at the cellular or invertebrate level. However, the usage of biomolecule-based nanomaterials as contrast agents for EDF-HSI is highly challenging because these nanomaterials usually lack the characteristic optical scattering properties that can be readily distinguished from the scattering signals of organisms. Herein, we report peptide nanoparticles fabricated through covalent self-assembly of dipeptides and genipin for EDF-HSI in vitro and in vivo. Two dipeptides with either C-terminal amidation or not were used as the building blocks for covalent self-assembly. Significantly, the optical scattering intensity of the resulting nanoparticles is enhanced by the amidation. The amidation also enhances the stability of the peptide nanoparticles both in solutions and inside cells or organisms due to inhibition of electrostatic repulsion among the building blocks. Therefore, the nanoparticles fabricated from the dipeptide with uncharged C-terminal amide can be facilely visualized and tracked inside cells and nematodes through EDF-HSI. These results demonstrate that peptide nanomaterials can be endowed with optical scattering properties suitable for EDF-HSI, providing a promising approach for visualization and analysis of the activities of biomaterials in vitro and in vivo.
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