期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 118, 期 7, 页码 2585-2596出版社
WILEY
DOI: 10.1002/bit.27777
关键词
13C solid‐ state NMR; artificial protein; biomaterial; peptide aptamer; transgenic silk fibroin
资金
- Japan Society for the Promotion of Science [23240135, JP19K05609]
- Grants-in-Aid for Scientific Research [23240135] Funding Source: KAKEN
In this study, silk fibroin (SF) from Bombyx mori was endowed with reversible binding ability to titanium surfaces by embedding a titanium binding motif. The functionalized SF (TiBP-SF) can accelerate the differentiation of bone cells, providing a new method for endowing titanium materials with osseointegration functionality in potential medical applications.
Silk fibroin (SF) from Bombyx mori has superior properties as both a textile and a biomaterial, and has been used to functionalize the surfaces of various medical inorganic materials including titanium (Ti). In this study, we endowed SF with reversible binding ability to Ti by embedding a titanium binding motif (minTBP-1 and RKLPDA). Artificial SF proteins were first created by conjugating gene cassettes for SF motif (AGSGAG) and minTBP-1 motif with different ratios, which have been shown to bind reversibly to Ti surfaces in quartz crystal microbalance analyses. Based on these results, the functionalized SF (TiBP-SF) containing the designed peptide [TS[(AGSGAG)(3)AS](2)RKLPDAS](8) was prepared from the cocoon of transgenic B. mori, which accelerates the ossific differentiation of MC3T3-E1 cells when coated on titanium substrates. Thus, TiBP-SF presents an alternative for endowing the surfaces of titanium materials with osseointegration functionality, which would allow the exploration of potential applications in the medical field.
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