Article
Biochemical Research Methods
Regina T. Glatz, H. Ceren Ates, Hasti Mohsenin, Wilfried Weber, Can Dincer
Summary: Clinical assessment often requires the measurement of multiple analytes or samples to improve the accuracy of diagnosis and therapy. In this study, a microfluidic multiplexed electrochemical biosensor technology (BiosensorX) was developed, allowing simultaneous detection of multiple analytes or samples within a single microfluidic channel. The vertical design of the BiosensorX chip was chosen for its ease of use and superior fluidic behavior. The feasibility of the BiosensorX for on-site testing was demonstrated with the measurement of meropenem antibiotics using an antibody-free beta-lactam assay. This multiplexed biosensor platform can be further extended for the detection of other anti-infective agents and biomarkers, as well as different sample types.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Quynh Anh Phan, Linh B. Truong, David Medina-Cruz, Can Dincer, Ebrahim Mostafavi
Summary: CRISPR-Dx offers broad enhancements compared to traditional nanobiosensors by utilizing the trans-cleavage activity of the CRISPR/Cas systems. However, the current singlestranded DNA/RNA reporters of CRISPR-Dx have poor stability and limited sensitivity. Research is shifting towards using the trans-cleavage activity of CRISPR/Cas effectors on metal nanoparticles to develop novel nanobiosensors with high accuracy, sensitivity, selectivity, and versatility.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Environmental Sciences
Esmail Pishbin, Fatemeh Sadri, Amin Dehghan, Mohammad Javad Kiani, Nader Hashemi, Iman Zare, Pegah Mousavi, Amid Rahi
Summary: Alzheimer's disease, a progressive neurological condition, requires early diagnosis for effective treatment management. This paper reviews recent advancements in detecting Alzheimer's disease related exosomal microRNAs using different methods such as electrochemical, fluorescent, and SPR. The use of microfluidic devices to isolate and detect these valuable biomarkers is also summarized. Challenges with the performance of novel technologies for isolating and detecting exosomal microRNAs are addressed.
ENVIRONMENTAL RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Jing Wang, Xifang Yang, Xueliang Wang, Wanhe Wang
Summary: CRISPR/Cas-based biosensors have been widely used for nucleic acid detection, but their applications for protein detection have been overlooked. Recent studies show that CRISPR/Cas-based biosensors not only analyze proteins, but also enhance the sensitivity and specificity of protein detection. However, there is a lack of comprehensive reviews and elucidation of the mechanisms of CRISPR/Cas-based biosensors for protein detection, which hinders the development of better protein biosensors.
BIOENGINEERING-BASEL
(2022)
Article
Chemistry, Analytical
Jitendra Gupta, Shahabe Saquib Abullais, Raed H. Althomali, Deysi Margoth Guanga Chunata, Sherzod Shukhratovich Abdullaev, Hanin E. Yeslam, Orynzhamal Sarsembenova, Montather F. Ramadan, Ali Alsalamy, Safa Alkhayyat
Summary: The role of infectious pathogens bacteria in severe human illnesses or great deaths cannot be denied. The modern lifestyle has exacerbated the issue of infectious pathogen bacteria. Utilizing the CRISPR/Cas system as a microbial defense system has attracted significant attention, and advancements in nanotechnology have led to more efficient probes and sensing approaches in portable biosensors for the analysis of pathogenic bacteria.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Peijie Shen, Zhenjun Si, Di Huang, Zhipeng Xu, Ziyi Wang, Mengjun Fang, Zhinan Xu
Summary: A system combining CRISPR Cas12a-based biosensors and commercial pregnancy test strips has been developed for point-of-care testing of SARS-CoV-2. The assay showed high sensitivity and specificity, with low reagent cost and simple operation procedure. This system provides an important supplement for the prevention and early diagnosis of infectious diseases in resource-limited situations.
Article
Chemistry, Physical
Songcheng Yu, Shengnan Cao, Sitian He, Kaixiang Zhang
Summary: DNA methylation is an important epigenetic characteristic that can result in heritable and revisable changes in phenotype without altering the DNA sequence. Aberrant methylation at specific loci has been linked to various diseases. The use of Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems as biosensors has shown potential for detecting locus-specific DNA methylation.
Review
Plant Sciences
Qiaoling Tang, Xujing Wang, Xi Jin, Jun Peng, Haiwen Zhang, Youhua Wang
Summary: CRISPR/Cas technology is an important global strategy for crop breeding to meet sustainable food demand. It has shown significant advances in gene function analysis and the generation of new germplasms with improved characteristics. Its applications in agriculture, such as crop domestication, yield improvement, and stress resistance enhancement, have great potential to transform the field of crop breeding.
Article
Chemistry, Analytical
Li Zhang, Xu Wang, Dongchen Liu, Yu Wu, Li Feng, Chunyan Han, Jiajia Liu, Ying Lu, Dmitriy V. Sotnikov, Youchun Xu, Jing Cheng
Summary: Respiratory tract infections, including COVID-19, have posed a serious threat to public health. A swing-assisted multiplexed analyzer for point-of-care respiratory tract infection testing (SMART) was proposed to achieve low-cost and multiplexed nucleic acid detection. By performing hand-swing movements, the SMART system simplified the distribution of samples into reaction chambers. It successfully detected multiple respiratory tract pathogens, including SARS-CoV-2 and respiratory bacteria, indicating its potential as a versatile tool in the diagnosis of respiratory tract infections.
Review
Chemistry, Analytical
Nazente Atceken, Defne Yigci, Berin Ozdalgic, Savas Tasoglu
Summary: Pathogen-specific point-of-care (PoC) diagnostic tests are crucial in fighting infectious diseases and epidemics. Molecular techniques, such as the combination of loop-mediated isothermal amplification (LAMP) and clustered regularly interspaced short palindromic repeat (CRISPR)-associated (CRISPR-Cas) technology, have the potential to overcome limitations in PoC diagnostics and produce tests with high sensitivity, specificity, and ease of use without the need for complex devices.
Article
Multidisciplinary Sciences
Tyler Sano, Ravipa Losakul, Holger Schmidt
Summary: Integrated optofluidic devices, combining microfluidics and photonic structures, have gained high interest for rapid biosensor devices. Researchers demonstrated a polydimethylsiloxane-based device with two on-chip optofluidic laser cavities, enabling simultaneous detection of multiple fluorescent targets.
SCIENTIFIC REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Midori Johnston, H. Ceren Ates, Regina T. Glatz, Hasti Mohsenin, Rosanne Schmachtenberg, Nathalie Goeppert, Daniela Huzly, Gerald A. Urban, Wilfried Weber, Can Dincer
Summary: This study proposes an electrochemical microfluidic multiplexed polymer-based biosensor combined with CRISPR/Cas-powered assays for low-cost and accessible point-of-care nucleic acid testing. The method allows simultaneous screening and identification of SARS-CoV-2 infections while bypassing reverse transcription and target amplification steps. Moreover, the study demonstrates the feasibility of combining different types of synthetic biology-driven assays on the same device, providing a promising on-site tool for rapid diagnosis and disease monitoring.
Review
Chemistry, Physical
Mohamed Sharafeldin, James F. Rusling
Summary: Rapid and accurate diagnoses are crucial for efficient healthcare, enabling early disease detection, avoiding unnecessary treatment, and improving outcomes. Electrochemical techniques offer user-friendly, sensitive, and low-cost assays to support clinical applications by analyzing relevant disease biomarkers. Multiplexed electrochemical assays provide more accurate and precise diagnostics compared to single biomarker assays, leveraging the inherent sensitivity and portability of electrochemistry.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Review
Chemistry, Analytical
Jianyu Han, Songqin Liu, Zhi Wang, Yafeng Wu
Summary: This review systematically summarizes the recent advances in micro/nanofluidic-electrochemical biosensors for in situ tumor cell analysis, including circulating tumor cells, biomarkers on tumor cells, and biomarkers secreted by tumor cells. The technology offers advantages of high sensitivity, easy integration, and low cost.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Food Science & Technology
Yunhao Lu, Hao Yang, Jinrong Bai, Qiang He, Ruijie Deng
Summary: Foodborne pathogenic infections have caused various problems in human life, and there is a need for advanced detection technologies. CRISPR/Cas-based biosensors have the potential to address the challenges of conventional assays such as insensitivity, long turnaround time, and complex pretreatments. This review focuses on the strategies of CRISPR/Cas-assisted diagnostics for foodborne pathogens, specifically discussing biosensing platforms based on fluorescence, colorimetric, (electro)chemiluminescence, electrochemical, and surface-enhanced Raman scattering detection. The review summarizes the detection principles for foodborne pathogenic bacteria, fungi, and viruses. The current challenges and technical barriers of these methods in broad application are also discussed, along with alternative solutions to improve the potential of CRISPR/Cas for food safety.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
H. Ceren Ates, Hasti Mohsenin, Christin Wenzel, Regina T. Glatz, Hanna J. Wagner, Richard Bruch, Nico Hoefflin, Sashko Spassov, Lea Streicher, Sara Lozano-Zahonero, Bernd Flamm, Rainer Trittler, Martin J. Hug, Maja Koehn, Johannes Schmidt, Stefan Schumann, Gerald A. Urban, Wilfried Weber, Can Dincer
Summary: Personalized antibiotherapy can maximize efficacy, minimize side effects, and prevent drug resistance. To better integrate therapeutic drug monitoring into clinical practice, a versatile biosensor with an antibody-free assay for on-site testing is introduced. This system offers rapid, low-cost, sample-independent, and multiplexed analysis, which can potentially shift the paradigm of one-size-fits-all strategy.
ADVANCED MATERIALS
(2022)
Article
Biophysics
Quynh Anh Phan, Linh B. Truong, David Medina-Cruz, Can Dincer, Ebrahim Mostafavi
Summary: CRISPR-Dx offers broad enhancements compared to traditional nanobiosensors by utilizing the trans-cleavage activity of the CRISPR/Cas systems. However, the current singlestranded DNA/RNA reporters of CRISPR-Dx have poor stability and limited sensitivity. Research is shifting towards using the trans-cleavage activity of CRISPR/Cas effectors on metal nanoparticles to develop novel nanobiosensors with high accuracy, sensitivity, selectivity, and versatility.
BIOSENSORS & BIOELECTRONICS
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Alara Altay, Robert Learney, Firat Guder, Can Dincer
Summary: Blockchain technology has sparked global discussions on digital decentralization to connect societies and economies. Through the connection of low-cost sensors in Internet of Things networks, automated measurements, data exchange, and decision-making can be achieved over decentralized networks, improving healthcare, product quality, and supply chain efficiency.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Gozde Kabay, Andreas Manz, Can Dincer
Summary: This perspective highlights the significance of nanotheranostic materials (NTMs) and the role of microfluidic systems in facilitating their clinical application. It also emphasizes the current research methods and existing issues in the development of NTMs.
Article
Multidisciplinary Sciences
Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V. Mymrikov, Nikolas M. Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra A. M. Fischer, Katharina E. Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse, Winfried Roemer, Thomas Ott
Summary: The organization of membranes in plants is mainly controlled by the cell wall and cytoskeleton, with some proteins, such as remorins, playing a role in protein and lipid organization. Symbiosis-specific SYMREM1 gene mutations disrupt membrane organization in root nodules. AlphaFold modeling and biochemical analyses reveal that SYMREM1 forms oligomers and may contribute to higher-order membrane scaffolding.
NATURE COMMUNICATIONS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Wilfried Weber, Martin Fussenegger
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Infectious Diseases
Noe Brasier, H. Ceren Ates, Juliane R. Sempionatto, Menino O. Cotta, Andreas F. Widmer, Jens Eckstein, Jorg Goldhahn, Jason A. Roberts, Wei Gao, Can Dincer
Summary: Bacterial antimicrobial resistance is a major cause of death worldwide, and therapeutic drug monitoring using wearable sensors can help mitigate this problem. Monitoring drug concentrations in non-invasively collectable biofluids can provide valuable information on the effectiveness of treatment at the site of infection.
LANCET INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Pamela E. Molinari, Adriana R. Krapp, Andrea Weiner, Hannes M. Beyer, Arun Kumar Kondadi, Tim Blomeier, Melina Lopez, Pilar Bustos-Sanmamed, Evelyn Tevere, Wilfried Weber, Andreas S. Reichert, Nora B. Calcaterra, Mathias Beller, Nestor Carrillo, Matias D. Zurbriggen
Summary: NADP(H) is a central metabolic hub that provides reducing equivalents to various pathways in living organisms. The genetically-encoded biosensor NERNST is able to interact with NADP(H) and estimate its redox state, allowing for the monitoring of NADP(H) dynamics in different organisms and potential applications in biochemical, biotechnological, and biomedical research.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Katrin Raute, Juliane Strietz, Maria Alejandra Parigiani, Geoffroy Andrieux, Oliver S. Thomas, Klaus M. Kistner, Marina Zintchenko, Peter Aichele, Maike Hofmann, Houjiang Zhou, Wilfried Weber, Melanie Boerries, Mahima Swamy, Jochen Maurer, Susana Minguet
Summary: There is currently no targeted therapy for patients with triple-negative breast cancer (TNBC). TNBC is enriched in breast cancer stem cells (BCSC), which play a key role in metastasis, chemoresistance, relapse, and mortality. y8 T cells hold great potential in immuno-therapy against cancer and might provide an approach to therapeutically target TNBC.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Maximilian Hoerner, Jan Becker, Rebecca Bohnert, Miguel Banos, Carolina Jerez-Longres, Vanessa Muehlhaeuser, Daniel Haerrer, Tin Wang Wong, Matthias Meier, Wilfried Weber
Summary: Hydrogels with adjustable mechanical properties have been developed to manipulate cell fate and function by converting optical signals into reversible changes in hydrogel mechanics. However, the limited dynamic range of addressable stiffness hampers their broader implementation. In this study, a photoreceptor-based hydrogel with reversibly adjustable stiffness is developed, enabling a wide range of stiffness from approximately 800 Pa to the sol state. The hydrogel's light-responsive mechanical properties are characterized and it is applied for the spatiotemporally controlled deposition of mammalian cells, which holds promise in overcoming spatial restrictions.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biochemistry & Molecular Biology
Marissa Russ, Anna K. Ehret, Maximilian Hoerner, Daniel Peschkov, Rebecca Bohnert, Vincent Idstein, Susana Minguet, Wilfried Weber, Bjoern F. Lillemeier, O. Sascha Yousefi, Wolfgang W. Schamel
Summary: The kinetics of ligand-receptor interaction can be experimentally and reversibly changed using optogenetics. Researchers have developed a method to control the interaction between a modified receptor and an engineered ligand using light. By expressing the SpyCatcher fused to a transmembrane domain, they successfully displayed PhyB as a membrane-bound protein and coupled it with ligands expressed on other cells. These cells, called opto-antigen presenting cells (opto-APCs), allow two-dimensional ligand-receptor interactions to be controlled by light stimulation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Materials Science, Biomaterials
Carolina Jerez-Longres, Marieta Gomez-Matos, Jan Becker, Maximilian Hoerner, Franz-Georg Wieland, Jens Timmer, Wilfried Weber
Summary: Encapsulated cell-based therapies involve genetically-modified cells embedded in a material to produce therapeutic agents at specific locations in the body. While promising, safety concerns such as cell escape and uncontrolled production of therapeutic agents still exist. This study develops a material-genetic interface as a safety switch for engineered mammalian cells, improving the safety and translation of cell therapies.
BIOMATERIALS ADVANCES
(2023)
Editorial Material
Oncology
Pierre V. M. Trehin, Geisler Munoz-Guamuro, Wilfried Weber
MOLECULAR THERAPY-ONCOLYTICS
(2023)
Article
Biotechnology & Applied Microbiology
Carmela Niederberger, Arthur Vermeersch, Flavia Davidhi, Collin Y. Ewald, George Havenith, Joerg Goldhahn, Can Dincer, Noe Brasier
Summary: This article proposes a sweat-and wearable-based test to determine biological age, which is a real-time, noninvasive, and clinically applicable aging test in humans. This test would enable users to monitor their aging process and actively manage their lifestyle and health.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
