A novel amphibian-derived peptide alleviated ultraviolet B-induced photodamage in mice

Although the application potential of amphibian skin-derived active peptides in alleviating ultraviolet B (UVB)-induced damage has attracted increasing attention, research remains in its infancy. In this study, a new peptide (OM-GL15, GLLSGHYGRASPVAC) was identified from the skin of the green odorous frog (Odorrana margaretae). Results showed that OM-GL15 scavenged free radicals (2,2' diazo bis 3 ethylbenzothiazoline-6-sulfonic acid and 1,1-diphenyl-2-trinitrophenylhydrazine) and reduced Fe3+ to Fe2+. Moreover, topical administration of OM-GL15 significantly alleviated UVB-induced skin photodamage in mice. Exploration of the underlying mechanisms further showed that OM-GL15 exerted antioxidant potency. Specifically, the peptide reduced the levels of lipid peroxidation and malondialdehyde and protected epidermal cells from UVB-induced apoptosis by inhibiting DNA damage via down-regulation of p53, caspase-3, caspase-9, and Box and up-regulation of Bcl-2. Our results highlight the potential application of amphibian skin-derived peptides in protection against UVB-induced photodamage and provide a novel peptide candidate for the development of anti-photodamage agents.

Automated summary

The peptide OM-GL15 derived from the skin of the green odorous frog showed antioxidant properties by scavenging free radicals, reducing Fe3+ to Fe2+, and alleviating UVB-induced skin photodamage. It also protected epidermal cells from UVB-induced apoptosis by inhibiting DNA damage and regulating the expression of apoptosis-related proteins. These findings suggest the potential application of amphibian skin-derived peptides for protection against UVB-induced photodamage.