Article
Infectious Diseases
Yaohui Xu, Xiao Zhou, Zenghai Jiang, Yaru Qi, Abdelaziz Ed-Dra, Min Yue
Summary: The study revealed that Salmonella strains isolated from chicken embryos in Henan province exhibited high resistance to ampicillin (66.67%) and sulfisoxazole (66.67%), with 67.5% of strains being multidrug resistant and 21.67% confirmed as ESBLs positive. Resistance was found to be serovar-dependent, with ESBLs positive strains showing more multi-resistance than ESBLs negative strains.
Article
Infectious Diseases
Sandra Sevilla-Navarro, Marta Cerda-Cuellar, Teresa Ayats, Jaume Jorda, Clara Marin, Santiago Vega
Summary: A study in dromedary camels in Tenerife, Spain found a prevalence of 5.5% for Salmonella, with a single serovar isolated. Genetic analysis showed low diversity, indicating camels may serve as a reservoir for Salmonella transmission. While Campylobacter was not detected in the study, mandatory control measures should be considered for zoonotic pathogens in camels due to their increasing popularity in tourism.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Immunology
Anisha M. Thanki, Steven Hooton, Natasha Whenham, Michael G. Salter, Mike R. Bedford, Helen V. Masey O'Neill, Martha R. J. Clokie
Summary: This study showed that adding a phage cocktail to chicken feed effectively reduced Salmonella colonization in chickens, with the optimal dose of phage being 10(5) PFU/day. By the end of the study, no Salmonella was detected in the group that received phage at this dose. Phage treatment also improved the growth performance of challenged birds.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Microbiology
Yasser M. Sanad, Nesreen H. Aljahdali, Bijay K. Khajanchi, Rajesh Nayak, Ashraf Khan, Steven L. Foley
Summary: Salmonella causes a large number of infections and deaths in the U.S. Javiana serotype strains from Arkansas show high antimicrobial resistance. Genetic analysis reveals the diversity and distribution of the strains. Salmonella persist in human populations for multiple years.
Article
Microbiology
Vasco T. M. Gomes, Luisa Z. Moreno, Ana Paula S. Silva, Siddhartha Thakur, Roberto M. La Ragione, Alison E. Mather, Andrea M. Moreno
Summary: This study assessed the contamination of Salmonella enterica in pork and poultry meat sold in retail markets in Sao Paulo, Brazil. The results showed the presence of S. enterica in both poultry and pork samples, with different antimicrobial resistance profiles and serotypes. The study also found high resistance rates to critically important antimicrobials in meat samples.
Article
Biochemistry & Molecular Biology
Jen-Fu Hsu, Jang-Jih Lu, Chih Lin, Shih-Ming Chu, Lee-Chung Lin, Mei-Yin Lai, Hsuan-Rong Huang, Ming-Chou Chiang, Ming-Horng Tsai
Summary: In this study, a new approach using CRISPR and RFLP analyses was utilized to study 103 CC17/III GBS isolates causing neonatal invasive diseases. It was found that GBS isolates with different CRISPR-RFLP patterns exhibited significant differences in occurrences of severe sepsis and meningitis. These findings suggest that a specific CRISPR-RFLP pattern could potentially be used as a new biomarker to predict meningitis and illness severity in CC17/III GBS infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Zunita Zakaria, Latiffah Hassan, Zawiyah Sharif, Norazah Ahmad, Rohaya Mohd Ali, Suraya Amir Husin, Norfitriah Mohamed Sohaimi, Shafini Abu Bakar, Bashiru Garba
Summary: This study investigated the pathogenicity, antimicrobial resistance, and multilocus sequence types of Salmonella enterica subspecies enterica serovar Enteritidis in the central region of peninsular Malaysia. The isolates showed multidrug-resistance features and had various virulence genes. The findings suggest potential risks to humans and livestock from this foodborne pathogen.
Article
Food Science & Technology
Xiaojie Qin, Linlin Xiao, Jiaming Li, Mingzhe Yang, Changying Yang, Qingli Dong
Summary: Antibiotic-resistant S. 1,4,[5],12:i:- isolates were first found in the poultry farm environment in China. These isolates exhibited high genetic diversity and carried various plasmid replicon types. One multidrug-resistant isolate carried multiple resistance genes and plasmids, suggesting that resistance genes may be horizontally transferred through the combination of plasmids.
FOOD QUALITY AND SAFETY
(2022)
Article
Microbiology
Cui Li, Yulong Wang, Yufeng Gao, Chao Li, Boheng Ma, Hongning Wang
Summary: This study characterized 75 Salmonella isolates from broilers or broiler products, finding that 80.00% of the strains were multidrug resistant, with the main resistance pattern being CN-AZM-AMP-AMC-CAZ-CIP-ATM-TE-SXT-FOS-C. Analysis of CRISPR arrays revealed 517 unique spacer sequences and 31 unique direct repeat sequences, leading to the development of a novel typing method.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Chenxing Liu, Kaiyong Yao, Daxi Ren, Yingping Xiao
Summary: This study investigated the prevalence and antimicrobial susceptibility patterns of Salmonella in retail meat samples from slaughterhouses in Hangzhou. The positive rates of Salmonella in pork, chicken, and goose samples were 21.1%, 10.4%, and 10.0% respectively. The analysis of antimicrobial susceptibility revealed high levels of resistance, particularly to Sulfamethoxazole.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Roberta Torres de Melo, Taciano dos Reis Cardoso, Phelipe Augusto Borba Martins Peres, Raquelline Figueiredo Braz, Guilherme Paz Monteiro, Daise Aparecida Rossi
Summary: The study analyzed virulence genes, phylogenetic relationships, biofilm formation index, and ultrastructure in S. Minnesota at different temperatures, as well as evaluated its resistance to biocidal agents. Results indicate S. Minnesota has the potential to cause disease in humans and adapt to adverse conditions, especially showing resistance to sodium hypochlorite. Cross-contamination and spread among strains suggest a connection to biofilm formation.
Article
Immunology
Mojgan Manzari, Fereshteh Fani, Masoud Alebouyeh, Alaleh Moaddeli, Marjan Rahnamaye Farzami, Maneli Amin Shahidi, Seyed Shahram Shekarforoush
Summary: This study investigated the frequency and characteristics of Salmonella isolates from food-producing animals and human diarrheal samples in Shiraz, Iran. The results showed high rates of multidrug resistance among the isolates, with specific Salmonella species circulating between different sources over time. Mutations in key genes and the presence of resistant genes were also identified in the isolates.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2022)
Article
Food Science & Technology
Charalampos Kotzamanidis, George Vafeas, Virginia Giantzi, Sofia Anastasiadou, Stavros Mygdalias, Andigoni Malousi, Ekateriniadou Loukia, Sergelidis Daniel, Antonios Zdragas
Summary: This study investigated the genetic diversity and relatedness of Staphylococcus aureus strains from clinical and subclinical mastitis cases in different ruminants using molecular typing techniques. The findings revealed varying degrees of genetic relatedness, SE gene profiles, and antimicrobial resistance levels among the isolates.
Article
Infectious Diseases
Haiyan Xu, Weibing Zhang, Kai Zhang, Yue Zhang, Zhenyu Wang, Wei Zhang, Yang Li, Qiuchun Li
Summary: The study found that S. Typhimurium was the predominant serotype among Salmonella strains in Nantong city, China, with a high rate of multi-drug resistance. S. Enteritidis and S. Rissen were major serotypes among patients, while S. London, S. Derby, and S. Meleagridis were significant serotypes among asymptomatic people.
BMC INFECTIOUS DISEASES
(2021)
Article
Agriculture, Dairy & Animal Science
Kevin M. Santana-Hernandez, Eligia Rodriguez-Ponce, Inmaculada Rosario Medina, Begona Acosta-Hernandez, Simon L. Priestnall, Santiago Vega, Clara Marin, Marta Cerda-Cuellar, Ana Marco-Fuertes, Teresa Ayats, Teresa Garcia Beltran, Pablo A. Lupiola-Gomez
Summary: The aim of this study was to investigate the invasive species Lampropeltis californiae as a reservoir of Salmonella and its ability to spread different clones of the bacterium with zoonotic potential into the environment, as well as study its antimicrobial resistance patterns in Gran Canaria (Spain). The main results showed a high diversity of Salmonella subsp. salamae strains with a high prevalence of resistance to antimicrobials of public health importance.
Article
Multidisciplinary Sciences
Osei Akoto, Salome Yakubu, Linda A. Ofori, Nesta Bortey-sam, Nathaniel O. Boadi, Jonathan Horgah, Lyndon N. A. Sackey
Summary: This study focused on heavy metal contamination in topsoil within a mining area in Nangodi, Ghana. The soil samples were analyzed for Hg, Pb, Cd, As, Cr, and Fe. The results classified the soil samples as moderately contaminated to heavily contaminated and identified the sources of the metals.
Article
Food Science & Technology
Charity Wiafe Akenten, Linda Aurelia Ofori, Neyaz Ahmed Khan, Joyce Mbwana, Nimako Sarpong, Juergen May, Thorsten Thye, Kwasi Obiri-Danso, Ellis Kobina Paintsil, Dennis Fosu, Richard Odame Philipps, Daniel Eibach, Ralf Krumkamp, Denise Dekker
Summary: This study aimed to determine the frequency, genetics, and antimicrobial resistance profiles of ESBL-producing E. coli in free-range poultry in Agogo, Ghana. Results showed that 56.2% of collected fecal samples were positive for ESBL-producing E. coli, with high resistance to tetracycline (93.8%) and trimethoprim-sulfamethoxazole (66.7%). The majority of ESBL-producing E. coli carried the bla(CTX-M-15) gene.
FOODBORNE PATHOGENS AND DISEASE
(2023)
Article
Agriculture, Dairy & Animal Science
Abdurrahman Hassan Jibril, Iruka N. Okeke, Anders Dalsgaard, John Elmerdahl Olsen
Summary: This study investigated the prevalence of Salmonella in hatchlings in Nigeria, with a focus on resistant strains. The study also analyzed the genetic relatedness between hatchling strains and strains from poultry farms. The results showed a high prevalence of Salmonella and a significant presence of multidrug-resistant strains.
Article
Multidisciplinary Sciences
Yaovi Mahuton Gildas Hounmanou, Agnes Wanyana, Stephen Alafi, Fred Wabwire-Mangen, Henrik Christensen, John Elmerdahl Olsen, Denis Karuhize Byarugaba
Summary: This study found that multidrug-resistant ESBL-producing Escherichia coli is a major cause of infections in hospitals and communities. The study suggests that ESBL genes can be transmitted between healthcare and community settings through discharged patients who are still colonized. The long-term transmission is sustained by the transfer of plasmids circulating across niches and disseminating related resistomes.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Alessandra Tata, Filippo Marzoli, Miriam Cordovana, Alessia Tiengo, Carmela Zacometti, Andrea Massaro, Lisa Barco, Simone Belluco, Roberto Piro
Summary: This study developed and validated a method to automatically serotype Legionella pneumophila and distinguish it from Legionella non-pneumophila using Fourier-transform infrared spectroscopy (FT-IR) and machine learning. Data validation strategies were employed to ensure consistent performance of the method across a large microbial population. The method achieved high accuracy on different test sets from European collections.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Sara Petrin, Lucas Wijnands, Elisa Beninca, Lapo Mughini-Gras, Ellen H. M. Delfgou-van Asch, Laura Villa, Massimiliano Orsini, Carmen Losasso, John E. E. Olsen, Lisa Barco
Summary: Whole genome sequencing (WGS) is a standard typing technique used for characterizing foodborne pathogens. This study aimed to determine if different genes can predict the probability of infection [P(inf)]. Results showed variability in P(inf) values among and within serovars, with no clear pattern observed. Additionally, significant associations between P(inf) and biofilm formation were found.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Xiao Fei, Qiuchun Li, Xinan Jiao, John Elmerdahl Olsen
Summary: This study identified genes and single nucleotide polymorphisms (SNPs) that affected the infection dynamics of Salmonella Pullorum in avian macrophages. Further studies of these genes may provide insights into the development of host-specific infections by S. Pullorum.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Alessia Tiengo, Massimiliano Orsini, Sara Petrin, Carmen Losasso, Alessandra Longo, Giulia Cento, Laura Ciot, Raffaella Ceruti, Veronica Cibin, Lisa Barco
Summary: This article reports the genome sequence of a Salmonella enterica subsp. enterica serovar Bispebjerg strain isolated from a turkey flock in 2011. Genome analysis revealed the strain's pathogenic potential due to antimicrobial resistance and a multitude of Salmonella pathogenicity islands and virulence factors.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Immunology
Xiao Fei, Casper Schroll, Kaisong Huang, Jens P. Christensen, Henrik Christensen, Sebastien Lemire, Mogens Kilstrup, Line E. Thomsen, Lotte Jelsbak, John E. Olsen
Summary: In this study, the researchers investigated the gene expression in the peritoneal cavity of hens infected with Salmonella enterica serovar Gallinarum. They found specific up-regulation of genes associated with virulence and macrophage survival in S. Gallinarum. Furthermore, the study showed that host specificity was characterized by metabolic finetuning and unique expression of virulence associated pathways.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Ana Herrero Fresno, Anna Luiza Farias Alencar, Gang Liu, Mathilde Weinreich Wridt, Freja Bylling Andersen, Hanne Skovsgaard Pedersen, Henrik Laessoe Martin, Soren Saxmose Nielsen, Soren Aabo, John Elmerdahl Olsen, Annette Nygaard Jensen
Summary: This study aimed to investigate the effect of feeding milk fermented with a combination of four probiotic bacterial strains on reducing the occurrence of calf-diarrhoea. The results showed that this feeding approach did not significantly reduce the prevalence of diarrhoea but had a detrimental effect on daily-weight-gain.
VETERINARY MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Charity Wiafe Akenten, Neyaz Ahmed Khan, Joyce Mbwana, Ralf Krumkamp, Dennis Fosu, Ellis Kobina Paintsil, Kennedy Gyau Boahen, James Osei-Mensa, Oumou Maiga-Ascofare, Juergen May, Kwasi Obiri-Danso, Richard Odame Phillips, Linda Aurelia Ofori, Denise Dekker
Summary: We provide data on the faecal carriage, phenotypic resistance patterns, and gene variation of ESBL-EC and ESBL-KP among children in the Agogo region of Ghana. The results showed a high carriage rate of ESBL-EC and ESBL-KP, with no significant difference in prevalence between children with and without diarrhoea. Both ESBL-EC and ESBL-KP isolates showed over 70% resistance to tetracycline and sulfamethoxazole-trimethoprim.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Veterinary Sciences
Sophie Amalie Blirup-Plum, Henrik Elvang Jensen, Soren Saxmose Nielsen, Karen Pankoke, Mette Sif Hansen, Ken Steen Pedersen, Esben Ostergaard Eriksen, Jens Peter Nielsen, John Elmerdahl Olsen, Egle Kudirkiene, Lars Erik Larsen, Nicole Bakkegard Goecke, Kristiane Barington
Summary: This study investigated the associations between pathological manifestations and pathogens in pigs with and without post-weaning diarrhoea (PWD). The results showed that most of the pigs had gastric lesions, but gastric ulcers were less common in PWD pigs compared to pigs without PWD. Abnormal content in the colon was associated with PWD. No apparent associations were found between lesions and the various pathogens or a combination of these.
ACTA VETERINARIA SCANDINAVICA
(2023)
Article
Pharmacology & Pharmacy
Zaituni Msengwa, David Credo, Magesa Mafuru, James Mwesongo, Faith Philemon Mabiki, Beda John Mwang'onde, Madundo Mkumbukwa Mtambo, Lughano Jeremy Kusiluka, Robinson Hammerthon Mdegela, John Elmerdahl Olsen
Summary: This study investigated the antimicrobial activity and toxicity on red blood cells of compounds present in Commiphora swynnertonii extracts. The results showed that the extracts had moderate activity against Gram-positive bacteria and weak activity against Gram-negative bacteria. Furthermore, the extracts had low toxicity on red blood cells. Therefore, C. swynnertonii exhibits good antimicrobial activity and low toxicity.
RESEARCH JOURNAL OF PHARMACOGNOSY
(2023)
Article
Gastroenterology & Hepatology
Ellis Kobina Paintsil, Linda Aurelia Ofori, Charity Wiafe Akenten, Andreas E. Zautner, Joyce Mbwana, Neyaz Ahmed Khan, John P. A. Lusingu, Joseph Kaseka, Daniel T. R. Minja, Samwel Gesase, Anna Jaeger, Maike Lamshoeft, Juergen May, Kwasi Obiri-Danso, Ralf Krumkamp, Denise Dekker
Summary: This study investigated the frequency of potentially pathogenic Arcobacter species in commercial and smallholder farm animals in Ghana and Tanzania. The results showed high levels of multidrug-resistant Arcobacter in both countries, indicating the need for urgent attention and comprehensive strategies to control the spread of antimicrobial resistance in these pathogens.