期刊
BIOCHIP JOURNAL
卷 15, 期 2, 页码 195-203出版社
KOREAN BIOCHIP SOCIETY-KBCS
DOI: 10.1007/s13206-021-00019-y
关键词
SARS-CoV-2; SARS-CoV; Nucleoprotein (NP); Spike protein (SP); Immunoassay
资金
- National Research Foundation of Korea [NRF-2020R1A2B5B01002187, NRF-2020R1A5A101913111]
- National Research Foundation of Korea [4199990514159] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study purified antibodies against SARS-CoV-2 NP from pig serum in two steps, demonstrating enhanced specificity through immunoassays and estimating binding constants using a biosensor. Competitive assays showed the potential of these antibodies to detect SARS-CoV-2 and differentiate it from other related coronaviruses in viral fluids.
Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants (K-d) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
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