Local Deuteration Enables NMR Observation of Methyl Groups in Proteins from Eukaryotic and Cell‐Free Expression Systems

Improved strategy for isoleucine 1H/13C methyl labeling in Pichia pastoris

(2019) Rustam Ali et al.   JOURNAL OF BIOMOLECULAR NMR

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins

(2018) Mariya Lazarova et al.   ACS Chemical Biology

Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system

(2018) Yutaka Kofuku et al.   JOURNAL OF BIOMOLECULAR NMR

Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems

(2018) Saeko Yanaka et al.   JOURNAL OF BIOMOLECULAR NMR

Investigating the Dynamics of Destabilized Nucleosomes Using Methyl-TROSY NMR

(2018) Julianne L. Kitevski-LeBlanc et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies

(2018) Lindsay Clark et al.   JOURNAL OF BIOMOLECULAR NMR

Unraveling self-assembly pathways of the 468-kDa proteolytic machine TET2

(2017) Pavel Macek et al.   Science Advances

Improvement of Ligand Affinity and Thermodynamic Properties by NMR-Based Evaluation of Local Dynamics and Surface Complementarity in the Receptor-Bound State

(2016) Yumiko Mizukoshi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Sensitive proton-detected solid-state NMR spectroscopy of large proteins with selective CH3 labelling: application to the 50S ribosome subunit

(2016) Vilius Kurauskas et al.   CHEMICAL COMMUNICATIONS

Activation of the A2A adenosine G-protein-coupled receptor by conformational selection

(2016) Libin Ye et al.   NATURE

Conductance of P2X4purinergic receptor is determined by conformational equilibrium in the transmembrane region

(2016) Yuichi Minato et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

β 2 -Adrenergic Receptor Conformational Response to Fusion Protein in the Third Intracellular Loop

(2016) Matthew T. Eddy et al.   STRUCTURE

A Dynamic molecular basis for malfunction in disease mutants of p97/VCP

(2016) Anne K Schuetz et al.   eLife

Mapping Monoclonal Antibody Structure by 2D 13C NMR at Natural Abundance

(2015) Luke W. Arbogast et al.   ANALYTICAL CHEMISTRY

Identification of a Conformational Equilibrium That Determines the Efficacy and Functional Selectivity of the μ-Opioid Receptor

(2015) Junya Okude et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells

(2015) Agnieszka Sitarska et al.   JOURNAL OF BIOMOLECULAR NMR

Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris

(2015) Lindsay Clark et al.   JOURNAL OF BIOMOLECULAR NMR

Propagation of conformational changes during μ-opioid receptor activation

(2015) Rémy Sounier et al.   NATURE

Functional Dynamics of Deuterated β2-Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy

(2014) Yutaka Kofuku et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression

(2014) Rasmus Linser et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility

(2013) Manuel Etzkorn et al.   STRUCTURE

Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

(2010) Pierre Gans et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor

(2010) Michael P. Bokoch et al.   NATURE

Dynamic Regulation of Archaeal Proteasome Gate Opening As Studied by TROSY NMR

(2010) T. L. Religa et al.   SCIENCE