期刊
ACS NANO
卷 15, 期 4, 页码 7040-7052出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.1c00135
关键词
smartphone; optogenetics; engineering bacteria; gut; ulcerative colitis; optotheranostic nanosystem
类别
资金
- National Key Research and Development Program of China [2019YFA0906500, 2017YFA0205104]
- National Natural Science Foundation of China [31971300, 817719709, 51873150]
- Tianjin Natural Science Foundation [19JCYBJC28800]
- Young Elite Scientists Sponsorship Program by Tianjin
- Key Project of Tianjin Foundational Research (JingJinJi) Program, China [19JCZDJC64100]
A study developed an optotheranostic nanosystem for self-diagnosis and long-acting mitigation of ulcerative colitis (UC). The system utilized smartphone optical sensing for disease prescreening and an optogenetic engineered bacteria system for real-time intervention. Treatment with IL-10-secreting EcN effectively suppressed intestinal inflammatory response and protected intestinal mucosa in UC mice.
Ulcerative colitis (UC) is a relapsing disorder characterized by chronic inflammation of the intestinal tract. However, the home care of UC based on remote monitoring, due to the operational complexity and time-consuming procedure, restrain its widespread applications. Here we constructed an optotheranostic nanosystem for self-diagnosis and long-acting mitigations of UC at home. The system included two major modules: (i) A disease prescreening module mediated by smartphone optical sensing. (ii) Disease real-time intervention module mediated by an optogenetic engineered bacteria system. Recombinant Escherichia coli Nissle 1917 (EcN) secreted interleukin-10 (IL-10) could down-regulate inflammatory cascades and matrix metalloproteinases; it is a candidate for use in the therapeutic intervention of UC. The results showed that the Detector was able to analyze, report, and share the detection results in less than 1 min, and the limit of detection was 15 ng.mL(-1). Besides, the IL-10-secreting EcN treatment suppressed the intestinal inflammatory response in UC mice and protected the intestinal mucosa against injury. The optotheranostic nanosystems enabled solutions to diagnose and treat disease at home, which promotes a mobile health service development.
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