Article
Microbiology
Qin Yang, Yao Zhu, Stefan Schwarz, Lingli Wang, Wenyu Liu, Wenlin Yang, Tian Luan, Siguo Liu, Wanjiang Zhang
Summary: Tetracyclines are broad-spectrum antibiotics used in veterinary medicine and food animal production. This study identified tandem tetracycline resistance genes tet(61)-tet(58) on the novel plasmid pT4303, which can confer resistance to both Gram-positive and Gram-negative bacteria. The presence of multiple tet genes on the same plasmid suggests a potential vehicle for the dissemination of tetracycline resistance genes.
VETERINARY MICROBIOLOGY
(2021)
Article
Environmental Sciences
Xin Ma, Zhiren Yang, Tingting Xu, Mengqi Qian, Xuemei Jiang, Xiuan Zhan, Xinyan Han
Summary: This study found that long-term use of chlortetracycline can significantly reduce the diversity of gut microbiota in pigs, alter its structure, and increase the abundance of antibiotic resistance genes (ARGs) in feces.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Infectious Diseases
Kavita Yadav, Linnea Garoff, Douglas L. Huseby, Diarmaid Hughes
Summary: The study revealed significant phenotypic and genetic barriers to the fixation of additional RPP genes in E. coli, except for tet(Q).
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biology
Qipeng Cheng, Yanchu Cheung, Chenyu Liu, Edward Wai Chi Chan, Kwok Yin Wong, Rong Zhang, Sheng Chen
Summary: In this study, essential residues in TetX variants that mediate the evolution of the tetecycline-inactive Tet(X2) enzyme to the active forms were identified. Based on their amino acid sequences and functional features, TetX variants were classified into three classes. Furthermore, the study showed the origin and transmission of different TetX variants, which may contribute to better clinical management of TetX-producing strains.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rupert Bassitta, Annika Nottensteiner, Johann Bauer, Reinhard K. Straubinger, Christina S. Hoelzel
Summary: This study quantified the absolute concentrations and relative abundances of antimicrobial resistance (AMR) genes in manure from organic and conventional farms. The results showed that the relative abundances of AMR genes were higher in conventional farms compared to organic farms. The use of antibiotics was linked to the relative abundance of AMR genes. However, the absolute concentrations of AMR genes were comparable between fertilizers from organic and conventional farms.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Yiping Wu, Ruowen He, Mingyang Qin, Yanxian Yang, Jieyun Chen, Yu Feng, Xiaoxue Liang, Wenbin Deng, Xin Ding, Li-Na Qin, Kang Liao, Yongqiang Yang, Guo-Bao Tian
Summary: We identified two tet(X4)-positive E. cloacae isolates and found that the pTECL_2-190k-tetX4-like plasmid has been widely disseminated across various species of Enterobacteriaceae. By analyzing 85 tet(X4)-carrying plasmids, we determined that these plasmids have multiple replicon types, including IncFIA, IncHI1A, and IncHI1B.
MICROBIOLOGY SPECTRUM
(2022)
Article
Environmental Sciences
Side Yang, Lixia Zhao, Xingping Chang, Zheng Pan, Bin Zhou, Yang Sun, Xiaojing Li, Liping Weng, Yongtao Li
Summary: The study demonstrated that earthworms can significantly accelerate the degradation of chlortetracycline and its metabolites in soil, reducing the abundance of antibiotic resistance genes. Additionally, earthworms altered the structure of soil bacterial community and promoted potential chlortetracycline degraders.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Medicine, General & Internal
Lei Xu, Yonglin Zhou, Sen Niu, Zhiying Liu, Yinuo Zou, Yanan Yang, Haihua Feng, Dejun Liu, Xiaodi Niu, Xuming Deng, Yang Wang, Jianfeng Wang
Summary: This study identified plumbagin as an effective broad-spectrum inhibitor of Tet(X3)/Tet(X4), which can synergistically kill bacteria producing these resistant genes when used in combination with tetracyclines.
Article
Engineering, Environmental
Yulin Fu, Yiqiang Chen, Dejun Liu, Dawei Yang, Zhihai Liu, Yingyu Wang, Jiayi Wang, Xueyang Wang, Xiangyue Xu, Xing Li, Junjia He, Junyao Jiang, Weishuai Zhai, Lingli Huang, Tao He, Xi Xia, Chang Cai, Yang Wang, Haiyang Jiang
Summary: The study found that tet(X4), tmexCD1-toprJ1, and other tigecycline resistance genes were more abundant in chicken feces, indicating a higher risk of TRG propagation in chicken feces. Positive correlations between ISCR2/IS26 and TRGs further demonstrated the potential transfer and mobilization of these resistance genes.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Zhen Bi, Ge Song, Xiaoming Sun
Summary: The responses of anammox consortia to antibiotics SDZ and CTC were evaluated in terms of general performance, microbial activity, diversity and abundance of antibiotic resistance genes (ARGs). The results showed that the consortia had great resistance to SDZ but were heavily affected by CTC. The presence of ARGs, particularly in Ca. Brocadia bacteria, played a significant role in the resistance mechanism.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Genetics & Heredity
Antony T. Vincent, Romain P. Bergeron, Laurie C. Piche, David Prado, Linda Saucier
Summary: This study analyzes the genomic level of the tetracycline-resistant strain B. thermosphacta BT469 to understand the molecular determinants responsible for this resistance. Three plasmids have been identified in the strain, with pBT469-2 being unique to the BT469 strain. Similar plasmid sequences have also been found in other bacterial genera, suggesting a diverse family of plasmids present in multiple bacterial genera.
Article
Biotechnology & Applied Microbiology
Sinan Zhang, Jihong Wang
Summary: Biochar-immobilized microorganism technology using Chinese medicine residue waste substrate is an effective method to remove antibiotic contamination from water bodies. The study optimized the conditions for microbial immobilization by biochar and analyzed the mechanisms of chlortetracycline removal by the complex, providing a new idea for water purification.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Genetics & Heredity
Vesna Milanovic, Antonietta Maoloni, Luca Belleggia, Federica Cardinali, Cristiana Garofalo, Cristiana Cesaro, Lucia Aquilanti, Andrea Osimani
Summary: The presence of antibiotic resistance genes, especially tet(M), in surstromming, a traditional Swedish fermented herring, may be due to selective pressure from the natural marine environment on the gut microbiota of herrings used as raw materials. However, the contribution of the manufacturers to the contamination cannot be excluded.
Article
Environmental Sciences
Vanesa Santas-Miguel, Laura Rodriguez-Gonzalez, Avelino Nunez-Delgado, Esperanza Alvarez-Rodriguez, Montserrat Diaz-Ravina, Manuel Arias-Estevez, David Fernandez-Calvino
Summary: The presence of antibiotics in soil can increase bacterial community tolerance to these pollutants, especially at high concentrations. Soils with low organic matter content have a higher risk, but this risk decreases over time.
Review
Infectious Diseases
Sabrina Lunara Santos Pavelquesi, Ana Carolina Almeida de Oliveira Ferreira, Angeislenie Ricelle Magalhaes Rodrigues, Calliandra Maria de Souza Silva, Daniela Castilho Orsi, Izabel Cristina Rodrigues da Silva
Summary: The study revealed high resistance rates of Salmonella to tetracyclines and sulfonamides, with poultry and pork being the most frequent samples used to isolate Salmonella. The most frequently detected tetracycline resistance genes in Salmonella were tetA and tetB, while sul1 was the most common sulfonamide resistance gene. These genes are associated with plasmids, transposons, or both, and have potential for transfer to other bacteria, environments, animals, and humans.
Article
Agriculture, Dairy & Animal Science
Ruonan Chen, Qingpo Chu, Chunyan Shen, Xian Tong, Siyuan Gao, Xinpeng Liu, Bo Zhou, Allan P. Schinckel
Article
Agriculture, Dairy & Animal Science
Chunyan Shen, Xian Tong, Ruonan Chen, Siyuan Gao, Xinpeng Liu, Allan P. Schinckel, Yuan Li, Feilong Xu, Bo Zhou
APPLIED ANIMAL BEHAVIOUR SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
Xian Tong, Chunyan Shen, Ruonan Chen, Siyuan Gao, Xinpeng Liu, Allan P. Schinckel, Bo Zhou
Article
Agriculture, Dairy & Animal Science
Yuan Li, Feilong Xu, Xian Tong, Ruonan Chen, Chunyan Shen, Tingting Liang, Qingpo Chu, Bo Zhou
Article
Biology
Jing Zhao, Siyuan Gao, Yanli Guo, Qinglei Xu, Mingzheng Liu, Chunlei Zhang, Meng Cheng, Xianle Zhao, Allan P. Schinckel, Bo Zhou
Summary: This study evaluated the impact of the dopamine receptor D2 gene on aggressive behavior in pigs and identified a functional single-nucleotide polymorphism that affects the binding of transcriptional factors. The findings shed light on the genetics and neurophysiology of aggressive behavior in pigs, providing insight towards controlling this behavior.
Article
Agriculture, Dairy & Animal Science
Yanli Guo, Jing Zhao, Qinglei Xu, Siyuan Gao, Mingzheng Liu, Chunlei Zhang, Allan P. Schinckel, Bo Zhou
Summary: This study identified functional single nucleotide polymorphisms of the porcine SLC6A4 gene associated with aggressive behavior in pigs after mixing. miR-671-5p and MAZ were found to mediate the expression level of SLC6A4 in pigs, suggesting a possible molecular mechanism for regulating aggressive behavior.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Siyuan Gao, Jing Zhao, Qinglei Xu, Yanli Guo, Mingzheng Liu, Chunlei Zhang, Allan P. Schinckel, Bo Zhou
Summary: Porcine miR-20b and miR-31 target HSD17B14 gene, and miR-31 also targets FSHR gene to regulate the metabolism and apoptosis of porcine ovarian GCs.
Article
Cell Biology
Qinglei Xu, Jing Zhao, Yanli Guo, Mingzheng Liu, Allan P. Schinckel, Bo Zhou
Summary: Pigs are important biomedical model animals for the study of human neurological diseases. Aggressive behavior in weaned pigs can have negative effects on animal welfare and growth performance. The ARHGAP24 gene has been identified as playing a critical role in the aggressive behavior of pigs, and specific SNPs have been found to be associated with this trait. The identification of these SNPs provides valuable molecular markers for genetic improvement programs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qing-Lei Xu, Chang Liu, Xiao-Jian Mo, Meng Chen, Xian-Le Zhao, Ming-Zheng Liu, Shu-Bai Wang, Bo Zhou, Cheng-Xin Zhao
Summary: This study evaluated the potential effects of adding acidifiers to the drinking water on the growth performance, complete blood count, antioxidant indicators, and diversity of gastrointestinal microbiota for weaned pigs. The results showed that supplementing the drinking water with acidifiers improved the growth performance, immunity, antioxidant capacity, and intestinal flora of weaned pigs.
Article
Biology
Mingzheng Liu, Qinglei Xu, Jing Zhao, Yanli Guo, Chunlei Zhang, Xiaohuan Chao, Meng Cheng, Allan P. Schinckel, Bo Zhou
Summary: This study investigates the roles of lincRNAs in the estrus expression of gilts and reveals their associations with ovarian steroid hormone and follicular development. The study identifies differentially expressed lincRNAs in the follicular tissues of Large White and Mi gilts and constructs PPI and ceRNA networks related to estrus expression. The findings suggest that lincRNAs and their target genes may impact estrus expression.
Article
Agronomy
Weiping Ao, Meng Cheng, Yanxu Chen, Jipeng Sun, Chunlei Zhang, Xianle Zhao, Mingzheng Liu, Bo Zhou
Summary: The supplementation of fermented apple pomace has beneficial impacts on the growth, plasma biochemistry and immune indicators, and gut microbiota of weaned pigs.
Article
Biochemistry & Molecular Biology
Chunlei Zhang, Huan Yang, Qinglei Xu, Mingzheng Liu, Xiaohuan Chao, Jiahao Chen, Bo Zhou, Yang Liu
Summary: This study investigates the interplay between copy number variation (CNV), gene expression changes, and aggressive behavior in pigs. The results suggest that CNVs affecting the SLCO3A1 gene may influence gene expression through a dosage effect, highlighting the potential of SLCO3A1 as a candidate gene associated with aggressive traits in pig breeding programs.
Article
Biochemistry & Molecular Biology
Siyuan Gao, Ruixin Tao, Xian Tong, Qinglei Xu, Jing Zhao, Yanli Guo, Allan P. Schinckel, Bo Zhou
Article
Agriculture, Dairy & Animal Science
Janaina Cristina da Silva Maciel de Souza, Laya Kannan Silva Alves, Eduarda Buck Bernardes Guimaraes, Gabriela dos Santos Madella, Bruno Braga Carnino, Emilye Izabele Cristina de Moraes, Pedro Ventura Dibo, Nicolas Carvalho Braga, Johnny Maciel de Souza, Bo Zhou, Cesar Augusto Pospissil Garbossa
JOURNAL OF ANIMAL BEHAVIOUR AND BIOMETEOROLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Qinxue Gao, Qingpo Chu, Mingzheng Liu, Siyuan Gao, Qinglei Xu, Bo Zhou
Summary: The study revealed the functionality and differential expression mechanism of the porcine SULT1C1 gene promoter region, identifying two key single nucleotide polymorphisms that may influence gene expression levels and estrus expression in pigs.
ANIMAL SCIENCE JOURNAL
(2022)