期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 24, 期 5, 页码 4871-4881出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-016-8175-y
关键词
Green biocides; Cultural heritage; Bacillus sp.; Biosurfactants; Lipopeptides; Antifungal activity; Peptide synthetase
资金
- European Union through the European Regional Development Fund ALENTEJO (Regional Operational Programme of the Alentejo) [ALT20-03-0145-FEDER-000015, ALT20-03-0246-FEDER-000004]
Several biosurfactants with antagonistic activity are produced by a variety of microorganisms. Lipopeptides (LPPs) produced by some Bacillus strains, including surfactin, fengycin and iturin are synthesized nonribosomally by mega-peptide synthetase (NRPS) units and they are particularly relevant as antifungal agents. Characterisation, identification and evaluation of the potentials of several bacterial isolates were undertaken in order to establish the production of active lipopeptides against biodeteriogenic fungi from heritage assets. Analysis of the iturin operon revealed four open reading frames (ORFs) with the structural organisation of the peptide synthetases. Therefore, this work adopted a molecular procedure to access antifungal potential of LPP production by Bacillus strains in order to exploit the bioactive compounds synthesis as a green natural approach to be applied in biodegraded cultural heritage context. The results reveal that the bacterial strains with higher antifungal potential exhibit the same morphological and biochemical characteristics, belonging to the genera Bacillus. On the other hand, the higher iturinic genetic expression, for Bacillus sp. 3 and Bacillus sp. 4, is in accordance with the culture antifungal spectra. Accordingly, the adopted methodology combining antifungal screening and molecular data is represent a valuable tool for quick identification of iturin-producing strains, constituting an effective approach for confirming the selection of lipopeptides producer strains.
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