Article
Medical Laboratory Technology
Ze Zhou, Suk Hang Cheng, Spencer C. Ding, Macy M. S. Heung, Tingting Xie, Timothy H. T. Cheng, W. K. Jacky Lam, Wenlei Peng, Jeremy Y. C. Teoh, Peter K. F. Chiu, Chi-Fai Ng, Peiyong Jiang, K. C. Allen Chan, Rossa W. K. Chiu, Y. M. Dennis Lo
Summary: The study reveals that urinary cfDNA has jagged ends, with a higher index compared to plasma DNA, particularly in patients with bladder cancer showing lower jagged end indexes. This suggests that jagged ends of urinary DNA could potentially serve as a new biomarker for bladder cancer detection.
CLINICAL CHEMISTRY
(2021)
Article
Oncology
Fernando Lozano, Carles X. X. Raventos, Albert Carrion, Carme Dinares, Javier Hernandez, Enrique Trilla, Juan Morote
Summary: The urine biomarker XBM can predict early high-risk recurrences in non-muscle invasive bladder cancer (NMIBC), but it has low sensitivity and cannot replace cystoscopy and washing cytology.
Article
Multidisciplinary Sciences
Patricia J. J. Brooks, Ethan Z. Z. Malkin, Steven De Michino, Scott V. V. Bratman
Summary: Saliva is an emerging source of disease biomarkers, particularly for cancers of the head and neck. Currently, there are no standardized methodologies for the collection and isolation of saliva for DNA analysis. In this study, various saliva collection receptacles and DNA purification techniques were evaluated, and optimal techniques for isolating DNA from saliva were defined. The study also demonstrated the stability of human papillomavirus (HPV) DNA in saliva samples and its reliable detection in patients with HPV-positive head and neck cancer. These findings contribute to the development of liquid biopsy-based cancer detection.
Article
Biochemistry & Molecular Biology
Raquel Carrasco, Mercedes Ingelmo-Torres, Ascension Gomez, Ramon Trullas, Fiorella L. Roldan, Tarek Ajami, Davinia Moreno, Leonardo Rodriguez-Carunchio, Antonio Alcaraz, Laura Izquierdo, Lourdes Mengual
Summary: Cell-free DNA (cfDNA) has emerged as a valuable biomarker for monitoring the prognosis of muscle-invasive bladder cancer (MIBC) patients. In particular, cfDNA levels and circulating tumor DNA (ctDNA) status after 4 months of radical cystectomy (RC) can predict tumor progression and clinical outcomes for these patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Peiyong Jiang, Y. M. Dennis Lo
Summary: A new assay for cell-free DNA fragmentation enhances the prediction of gene expression in liquid biopsies.
NATURE BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Stefan Harsanyi, Zuzana Varchulova Novakova, Katarina Bevizova, Lubos Danisovic, Stanislav Ziaran
Summary: Bladder cancer is the 10th most common cancer worldwide, with a variety of diagnostic methods available, including standard cystoscopy and urine cytology. Liquid biopsy as a new trend is proving to be almost equally effective in detecting bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Dai Koguchi, Kazumasa Matsumoto, Izuru Shiba, Takahiro Harano, Satoshi Okuda, Kohei Mori, Shuhei Hirano, Kazuki Kitajima, Masaomi Ikeda, Masatsugu Iwamura
Summary: Early detection of bladder cancer is crucial, but current screening methods have limited diagnostic accuracy. Liquid biopsy, including CTCs, ucfDNA, and umiRNA, has shown promise as a complementary diagnostic tool. However, more research is needed to validate its clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jae-Hak Ahn, Chan-Koo Kang, Eun-Mee Kim, Ah-Ram Kim, Aram Kim
Summary: Bladder cancer is the fourth most common cancer in men, with most cases being non-muscle-invasive. The high recurrence rate in non-muscle-invasive bladder cancer is a critical issue. Limited urine tests hinder the effective detection of superficial and small bladder tumors. Cystoscopy, although the gold standard for diagnosis, is associated with complications. Therefore, the development of urinary biomarkers is necessary for the detection of bladder cancer.
Article
Genetics & Heredity
Subhajyoti De
Summary: An effective strategy for early cancer detection requires a delicate balance in periodic screening, and genomic profiling of cell-free DNA is emerging as an attractive platform for sensitive detection of multiple types of cancer.
FRONTIERS IN GENETICS
(2021)
Review
Oncology
Camilla De Carlo, Marina Valeri, Devin Nicole Corbitt, Miriam Cieri, Piergiuseppe Colombo
Summary: This review summarizes the recent evidence on prognostic and predictive tissue biomarkers in non-muscle invasive bladder cancer (NMIBC), highlighting the importance of histological features and molecular events in predicting recurrence and progression. The review also emphasizes the potential of immunohistochemical evaluation as a cost-effective tool for clinical practice.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Weizhuo Wang, Xi Zhang, Silin Jiang, Peng Xu, Kang Chen, Kai Li, Fei Wang, Xiang Le, Ke Zhang
Summary: This study aimed to construct a prognostic model based on differential gene expression between muscular and non-muscular invasive bladder cancer (BCa), and to investigate the prognosis of BCa patients. BCa patient data from GEO and TCGA databases were analyzed, and single-cell sequencing and microarray data were used to validate the differential gene expression. The prognostic model showed accuracy and validity, and S100A9 may be a better prognostic marker and therapeutic target for BCa.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Qingxiao Fang, Ziming Yuan, Hanqing Hu, Weiyuan Zhang, Guiyu Wang, Xishan Wang
Summary: Through genome-wide analysis and validation, we identified four CpG sites (cg04486886, cg06712559, cg13539460, and cg27541454) as DNA methylation markers in colorectal cancer tissues. The diagnostic prediction model based on these markers can effectively distinguish colorectal cancer from polyp patients, and the md-score can robustly detect colorectal cancer. The cg27541454 may be a promising candidate noninvasive biomarker for early diagnosis of colorectal cancer.
CLINICAL EPIGENETICS
(2023)
Article
Oncology
Jinghua Zhang, Daofeng Dai, Junqiang Tian, Lifeng Li, Jing Bai, Yaping Xu, Zhiping Wang, Aifa Tang
Summary: This study demonstrated that using targeted sequencing to detect ctDNA in NMIBC patients can serve as a prognostic marker, revealing mutations in both tumor tissue DNA and plasma ctDNA. Patients with high heterogeneity in tumor DNA showed shorter disease-free survival, and a positive correlation was found between larger tumor size and molecular tumor burden index (mTBI) in ctDNA.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Douglas G. Ward, Laura Baxter, Sascha Ott, Naheema S. Gordon, Junhui Wang, Prashant Patel, Kim Piechocki, Lee Silcock, Chris Sale, Maurice P. Zeegers, K. K. Cheng, Nicholas D. James, Richard T. Bryan
Summary: Mutational analysis of urinary DNA can accurately detect and monitor bladder cancer, providing a noninvasive method for diagnosis and surveillance.
EUROPEAN UROLOGY ONCOLOGY
(2023)
Article
Oncology
Monica Cappetta, Lucia Fernandez, Lucia Brignoni, Nora Artagaveytia, Carolina Bonilla, Miguel Lopez, Manel Esteller, Bernardo Bertoni, Maria Berdasco
Summary: Human diversity poses a challenge in the development of robust worldwide biomarkers in oncology, with epigenetic variability linked to genetic backgrounds, lifestyles, and environmental exposures. In a study of sporadic breast cancer in Uruguay, a panel of 38 differentially methylated CpG positions was identified, with CYFIP1 methylation as a potential novel epigenetic biomarker for breast cancer in the Uruguayan population.
MOLECULAR ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Chak Kwong Cheng, Xiao Lin, Yujie Pu, Joyce Ka Yu Tse, Yu Wang, Cheng-Lin Zhang, Xiaoyun Cao, Chi Wai Lau, Juan Huang, Lei He, Jiang-Yun Luo, Yu-Tsung Shih, Song Wan, Chi Fai Ng, Li Wang, Ronald Ching Wan, Jeng-Jiann Chiu, Ting Fung Chan, Xiao Yu Tian, Yu Huang
Summary: This study utilized single-cell RNA sequencing to explore the transcriptional landscape and heterogeneity of dysfunctional endothelial cells in atherosclerotic aortae. SOX4 was identified as a novel marker for atherosclerosis and was shown to promote atherogenesis and endothelial-to-mesenchymal transition. Hyperlipidemia-associated cytokines and oscillatory blood flow were found to upregulate SOX4 expression, while the anti-diabetic drug metformin suppressed SOX4 levels in endothelial cells.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Endocrinology & Metabolism
Yan Hiu Athena Lee, Jeremy Man Ho Hui, Jeffrey Shi Kai Chan, Kang Liu, Edward C. Dee, Kenrick Ng, Pias Tang, Gary Tse, Chi Fai Ng
Summary: This study examined the associations between concurrent use of metformin and androgen deprivation therapy (ADT) and mortality risks in Asian, diabetic patients with prostate cancer (PCa). The results showed that the use of metformin concurrently with ADT was associated with lower risks of PCa-related mortality and all-cause mortality in these patients.
Letter
Medicine, General & Internal
Jeffrey Shi Kai Chan, Yan Hiu Athena Lee, Kang Liu, Jeremy Man Ho Hui, Edward Christopher Dee, Kenrick Ng, Danish Iltaf Satti, Pias Tang, Gary Tse, Chi Fai Ng
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Genetics & Heredity
Zirui Dong, Jicheng Qian, Tracy Sze Man Law, Matthew Hoi Kin Chau, Ye Cao, Shuwen Xue, Steve Tong, Yilin Zhao, Yvonne K. Kwok, Karen Ng, David Yiu Leung Chan, Peter K. -F. Chiu, Chi-Fai Ng, Cathy Hoi Sze Chung, Jennifer Sze Man Mak, Tak Yeung Leung, Jacqueline Pui Wah Chung, Cynthia C. Morton, Kwong Wai Choy
Summary: This study used Mate-pair genome sequencing to analyze 101 males with idiopathic infertility and identified clinically relevant structural variants in patients with non-obstructive azoospermia and severe oligozoospermia. The findings not only advance our understanding of the underlying mechanisms of male infertility but also impact clinical management.
Article
Urology & Nephrology
Jean de la Rosette, Jose Dominguez-Escrig, Kai Zhang, Jeremy Teoh, Eric Barret, Juan Casanova Ramon-Borja, Gordon Muir, Julia Bohr, Theo de Reijke, Chi-Fai Ng, Chi-Ho Leung, Rafael Sanchez-Salas, Pilar Laguna
Summary: The study aimed to evaluate the effect of focal vs extended irreversible electroporation on side effects, patient-reported quality of life, and early oncologic control for localized low- intermediate risk prostate cancer patients. The study found that focal and extended irreversible electroporation had similar safety profile, urinary function, and oncologic outcomes, but focal ablation demonstrated superior erectile function outcome.
JOURNAL OF UROLOGY
(2023)
Review
Oncology
Junjie Huang, Chi-Kwok Chan, Samuel Yee, Yunyang Deng, Yijun Bai, Sze Chai Chan, Man Sing Tin, Xianjing Liu, Veeleah Lok, Lin Zhang, Wanghong Xu, Zhi-Jie Zheng, Jeremy Yuen-Chun Teoh, Chi-Fai Ng, Martin C. S. Wong
Summary: This study is the first comprehensive meta-analysis examining the global prevalence of LUTS. It identified a high level of LUTS prevalence in the general population, with a higher burden in male subjects, older individuals, and the Asian population. There has been an increasing trend in the prevalence of LUTS since the 1990s.
PROSTATE CANCER AND PROSTATIC DISEASES
(2023)
Article
Surgery
Kai Zhang, Jeremy Teoh, Pilar Laguna, Jose Dominguez-Escrig, Eric Barret, Juan Casanova Ramon-Borja, Gordon Muir, Julia Bohr, Paula Pelechano Gomez, Chi-Fai Ng, Rafael Sanchez-Salas, Jean de la Rosette
Summary: This study found that focal and extended IRE ablation resulted in similar oncological outcomes in patients with localized low- or intermediate-risk prostate cancer. For some patients with intermediate-risk prostate cancer, who are still candidates for active surveillance, focal therapy may be a promising option.
Article
Oncology
Jeffrey Shi Kai Chan, Danish Iltaf Satti, Yan Hiu Athena Lee, Jeremy Man Ho Hui, Edward Christopher Dee, Kenrick Ng, Kang Liu, Gary Tse, Chi Fai Ng
Summary: Chinese patients with prostate cancer receiving ADT have an increasing risk of cardiovascular events despite a decreasing mortality rate.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Kaoru Murakami, Hideki Furuya, Kanani Hokutan, Steve Goodison, Ian Pagano, Runpu Chen, Cheng-Huang Shen, Michael W. Y. Chan, Chi Fai Ng, Takashi Kobayashi, Osamu Ogawa, Makito Miyake, Mark Thornquist, Yoshiko Shimizu, Kazukuni Hayashi, Zhangwei Wang, Herbert Yu, Charles J. Rosser
Summary: Bladder cancer (BCa) is a common cancer type with high recurrence rate. Plasminogen activator inhibitor-1 (PAI1) has been shown to play an important role in BCa development. This study evaluated the mutational status of PAI1 in a total of 660 subjects and identified two clinically relevant SNPs. Patients with at least one of these SNPs had a higher risk of recurrence and shorter survival. In vitro experiments demonstrated that one SNP increased the anti-apoptotic effect of PAI1, while another SNP led to uncontrolled cellular growth. These findings highlight the relevance and influence of these SNPs in bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Chris H. M. Wong, Ning Xu, Jasmine Lim, Kuo-kang Feng, Wayne K. W. Chan, Marco T. Y. Chan, Steven Ch Leung, Dong-ning Chen, Yun-zhi Lin, Peter K. F. Chiu, Chi Hang Yee, Jeremy Y. C. Teoh, Chiu-yuen Huang, Wei-sien Yeoh, Teng-aik Ong, Yong Wei, Chi-fai Ng
Summary: This study investigated the adverse effects of androgen deprivation therapy (ADT) on metabolic parameters in an Asian population. The results showed that ADT was associated with changes in glycemic profiles, lipid profiles, and body weight, which could be observed early in treatment and may persist beyond the first year.
Article
Urology & Nephrology
Hongda Zhao, Kang Liu, Stilianos Giannakopoulos, Emrah Yuruk, Geert De Naeyer, Mario Alvarez-Maestro, Chi-Fai Ng, Pilar Laguna, Jean De La Rosette, Jeremy Yuen-Chun Teoh
Summary: Based on a study of 2380 patients with upper tract urothelial carcinoma (UTUC), it was found that patients with a history of previous malignancy, especially non-urothelial cancer, had a higher risk of recurrence. Therefore, personalized follow-up plans and active treatment strategies should be considered for UTUC patients.
Article
Urology & Nephrology
Ja Hyeon Ku, Lui Shiong Lee, Tzu-Ping Lin, Eiji Kikuchi, Hiroshi Kitamura, Chi-Fai Ng, Junice Yi Siu Ng, Darren Ming-Chun Poon, Ravindran Kanesvaran, Ho Kyung Seo, Carmel Spiteri, Ee Min Tan, Ben Tran, Yuh-Shyan Tsai, Hiroyuki Nishiyama
Summary: This study highlights the considerable variation in risk stratification and management of non-muscle-invasive bladder cancer (NMIBC) in the Asia-Pacific region. It emphasizes the importance of developing practical algorithms to facilitate the recognition of NMIBC and standardize its treatment.
INTERNATIONAL JOURNAL OF UROLOGY
(2023)
Article
Urology & Nephrology
Peter Ka-Fung Chiu, Chun-Hong Chan, Alex Qinyang Liu, Sui-Yan Lau, Chi-Ho Leung, Yun-Sang Chan, Steffi Kar-Kei Yuen, Chi-Hang Yee, Jeremy Yuen-Chun Teoh, Wai-Lun Tang, Wing-Tat Poon, Chi-Fai Ng
Summary: This study aimed to investigate the effect of 5-alpha reductase inhibitor (5ARI) on prostate-specific antigen (PSA) and prostate health index (phi) in men with elevated PSA. The results showed that p2PSA and phi were reduced by approximately 55% and 34% in men on finasteride. A conversion factor of division by 0.66 is needed for phi in men on 5ARI.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2023)
Article
Oncology
Kang Liu, Hongda Zhao, Chi-Fai Ng, Jeremy Yuen-Chun Teoh, Pilar Laguna, Paolo Gontero, Iliya Saltirov, Jean de la Rosette
Summary: In patients with upper tract urothelial carcinoma, being overweight or obese is associated with a decreased risk of recurrence but does not affect overall survival or cancer-specific survival.
Meeting Abstract
Oncology
Lui Shiong Lee, Ravindran Kanesvaran, Eiji Kikuchi, Hiroshi Kitamura, Ja Hyeon Ku, Lin Tzu-Ping, Hiroyuki Nishiyama, Chi Fai Ng, Junice Yi Siu Ng, Darren Poon, Ho Kyung Seo, Carmel Spiteri, Ee Min Tan, Ben Tran, Yuh-Shyan Tsai
ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY
(2023)