期刊
MICROORGANISMS
卷 9, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms9030533
关键词
lignocellulosic biomass; bioprospecting; Hymenaea courbaril; Tamarindus indica; enzymes
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/25862-6, 2017/23989-9, 2017/09000-4, 2018/25898-3]
- National Institute of Science and Technology of Bioethanol [FAPESP 2008/57908-6, 2014/50884-5, 2018/07522-6]
- Conselho Nacional de Desenvolvimento Cientifico (CNPq) [574002/2008-1, 465319/2014-9, 301963/2017-7]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
- CNPq
This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis. The optimal conditions for enzyme production were found to be media supplemented with tamarind seeds under agitation. The research highlighted the strong influences of cultivation conditions on the production of enzymes by different fungi.
The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. alpha-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-alpha-L-arabinanase, beta-D-galactosidase, beta-D-glucosidase, beta-glucanase, beta-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-beta-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.
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