Article
Immunology
Derek E. Dimcheff, Andrew L. Valesano, Kalee E. Rumfelt, William J. Fitzsimmons, Christopher Blair, Carmen Mirabelli, Joshua G. Petrie, Emily T. Martin, Chandan Bhambhani, Muneesh Tewari, Adam S. Lauring
Summary: The study investigated the detection of total and subgenomic RNA of SARS-CoV-2 in nasopharyngeal swab samples and their relationship to symptom duration. Results showed that subgenomic RNA levels declined linearly with total RNA levels, with a constant relationship over the duration of symptoms. This suggests that subgenomic RNA may not provide additional information on infectivity beyond what can be determined by total RNA copies.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Joel D. Pearson, Daniel Trcka, Suying Lu, Sharon J. Hyduk, Mark Jen, Marie-Ming Aynaud, J. Javier Hernandez, Philippos Peidis, Miriam Barrios-Rodiles, Kin Chan, Jim Woodgett, Tony Mazzulli, Liliana Attisano, Laurence Pelletier, Myron I. Cybulsky, Jeffrey L. Wrana, Rod Bremner
Summary: The study evaluated the use of direct RT-qPCR and indirect methods with RNA extraction in COVID-19 testing. Results showed that the BGI system outperformed other systems in performance but at a higher cost, while the Norgen system was able to accurately detect samples with clinical Ct < 33 and save costs.
Article
Environmental Sciences
Golam Islam, Ashley Gedge, Linda Lara-Jacobo, Andrea Kirkwood, Denina Simmons, Jean-Paul Desaulniers
Summary: This study investigates the effects of five different parameters on the detection of the SARS-CoV-2 virus and fecal abundance indicator gene in wastewater samples. It provides method recommendations for reliably estimating the presence of the virus in diverse domestic wastewater samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Subhanjan Mondal, Nathan Feirer, Michael Brockman, Melanie A. Preston, Sarah J. Teter, Dongping Ma, Said A. Goueli, Sameer Moorji, Brigitta Saul, James J. Cali
Summary: Studies have shown that SARS-CoV-2 RNA can be detected in the feces of infected individuals, prompting investigation into using wastewater-based epidemiology to monitor the virus. A direct unbiased total nucleic acid (TNA) extraction method was developed to overcome the limitations of previous methods, allowing for a simple, rapid, and modular alternative for sample concentration and purification. This method showed potential for wastewater surveillance of SARS-CoV-2 and other infectious agents in communities.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Pharmacology & Pharmacy
Li-Jian Chen, Jie-Tao He, Ming Pan, Jia-Li Liu, Kai-Kai Zhang, Jia-Hao Li, Li-Bin Wang, Ling-Ling Xu, Yu-Kui Chen, Qin-Yao Zhang, Dong-Ri Li, Jing-Tao Xu, Xiao-Li Xie
Summary: The study found that antibiotics can attenuate METH-induced hepatotoxicity by regulating oxidative stress and the TLR4/MyD88/Traf6 axis, suggesting a potential protective role of antibiotics in preventing liver injury caused by METH.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Bingjian Cui, Chuncheng Liu, Chao Hu, Shengxian Liang
Summary: This study explores the physiological and gene expression characteristics of perennial ryegrass under cadmium (Cd) stress. High concentrations of Cd significantly inhibit the growth of ryegrass, while lower concentrations have minor effects. The activity levels of antioxidant enzymes increase in Cd-treated ryegrass leaves. The transcriptomic analysis identifies 1103 differentially expressed genes (DEGs) and reveals significant changes in molecular regulatory pathways.
Article
Plant Sciences
Michael Moebes, Heike Kuhlmann, Dmitri Demidov, Inna Lermontova
Summary: This study optimized the transcriptional RT-qPCR analysis of low-expression epigenetic genes in rapeseed, using KNL2 as an example. By combining various factors, the sensitivity of RT-qPCR for detecting the expression of BnKNL2A and BnKNL2C genes was significantly increased.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Medicine, General & Internal
Magdalena Komiazyk, Jaroslaw Walory, Aleksandra Kozinska, Izabela Wasko, Anna Baraniak
Summary: This study evaluated two RNA extraction methods and four commercial RT-qPCR assays for detecting SARS-CoV-2 in human upper respiratory tract specimens. The results demonstrated the significant impact of laboratory-selected diagnostic methods on the detection of SARS-CoV-2 with low viral loads in clinical specimens.
Article
Biotechnology & Applied Microbiology
C. Covas, A. Vaz, B. Almeida, J. Lourenco, G. Figueiredo, O. L. Franco, S. Mendo, T. Caetano
Summary: Secondary metabolites are bioactive compounds that are challenging to produce in the laboratory. This study used RNA-seq to investigate the cellular pathways of Pedobacter lusitanus NL19 modulated by high concentrations of peptone from casein (PC). The results showed that high PC concentrations significantly repressed the biosynthesis of biotin and the production of certain nonribosomal peptides, highlighting the effect of PC on gene expression and metabolic pathways.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Piyawat Pitikultham, Thitirat Putnin, Dechnarong Pimalai, Nuankanya Sathirapongsasuti, Chagriya Kitiyakara, Qiao Jiang, Baoquan Ding, Deanpen Japrung
Summary: In this study, a novel self-assembly approach based on rolling circle amplification and graphene oxide was proposed for the ultrasensitive detection of miRNA21 and miRNA16 in human saliva, which are oral cancer biomarkers. The proposed method exhibited ultrasensitive detection, a wide range of detection, and high selectivity, suggesting its potential application for the diagnosis and screening of early-stage oral cancer.
Article
Biotechnology & Applied Microbiology
Hyuna Park, Wonjong Jung, Hyeongseok Jang, Kak Namkoong, Kwon-Young Choi
Summary: This study developed a simple and rapid thermolysis method that can bypass the need for viral RNA extraction and purification. The method integrates thermolysis, RT, and digital PCR into a single chip for large-scale viral analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Kyungho Kim, Jothikumar Narayanan, Anindito Sen, Shankararaman Chellam
Summary: Iron electrocoagulation effectively removes and inactivates non-enveloped viruses under slightly acidic conditions, producing reactive oxygen species that can destroy viruses. It also causes morphological damages to viral capsids, showing promise as a technology for small-scale distributed water treatment.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marine Lambert, Abderrahim Benmoussa, Idrissa Diallo, Katheryn Ouellet-Boutin, Veronique Dorval, Nathalie Majeau, Charles Joly-Beauparlant, Arnaud Droit, Alain Bergeron, Bernard Tetu, Yves Fradet, Frederic Pouliot, Patrick Provost
Summary: A new family of unusually short RNAs, called doRNAs, has been discovered and found to potentially be more abundant and important than microRNAs. These doRNAs interact with hnRNPs in the cytoplasm and have differential expression in prostate cancer cells, possibly regulating cell migration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Juliana Vanessa Cavalcante Souza, Hazerral de Oliveira Santos, Anderson Brandao Leite, Marta Giovanetti, Rafael dos Santos Bezerra, Eneas de Carvalho, Jardelina de Souza Todao Bernardino, Vincent Louis Viala, Rodrigo Haddad, Massimo Ciccozzi, Luiz Carlos Junior Alcantara, Sandra Coccuzzo Sampaio, Dimas Tadeu Covas, Simone Kashima, Maria Carolina Elias, Svetoslav Nanev Slavov
Summary: Viral metagenomics is a valuable tool for identifying emerging and re-emerging viral pathogens in clinical samples with unknown etiology. This study showcased the utility of metagenomics in identifying arboviruses in samples with inconclusive amplification results, and identified Dengue virus type 2 and Chikungunya virus in the samples.
Article
Genetics & Heredity
Wenwu Chen, Haiming Ma, Biao Li, Fang Yang, Yu Xiao, Yan Gong, Zhi Li, Ting Li, Qinghua Zeng, Kang Xu, Yehui Duan
Summary: This study systematically analyzed circRNAs in the liver of Ningxiang pigs and revealed their expression profiles at different developmental stages. The results showed that these circRNAs are involved in pathways related to lipid metabolism and signal transduction, and a regulatory network was constructed.