Article
Biochemistry & Molecular Biology
Aniko Kun-Varga, Barbara Guban, Vanda Miklos, Shahram Parvaneh, Melinda Guba, Diana Szucs, Tamas Monostori, Janos Varga, Akos Varga, Zsolt Razga, Zsuzsanna Bata-Csorgo, Lajos Kemeny, Klara Megyeri, Zoltan Vereb
Summary: The proper functioning of mesenchymal stem cells (MSCs) is crucial for the body's homeostasis. Inflammation and infection can alter the function of MSCs, which in turn affects tissue regeneration and immunological status. This study aimed to investigate the response of adipose-derived MSCs (ADMSCs) to HSV infection in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Zilong Zhang, Jin Shang, Qinyan Yang, Zonglin Dai, Yuxin Liang, Chunyou Lai, Tianhang Feng, Deyuan Zhong, Haibo Zou, Lelin Sun, Yuhao Su, Su Yan, Jie Chen, Yutong Yao, Ying Shi, Xiaolun Huang
Summary: This study suggests that exosomes derived from human adipose mesenchymal stem cells (hADMSCs-Exo) have significant therapeutic effects on liver fibrosis and cirrhosis. The researchers found that hADMSCs-Exo can inhibit the proliferation of activated hepatic stellate cells, effectively suppress the expression of profibrogenic proteins and epithelial-to-mesenchymal transition (EMT), and improve liver function and reduce liver inflammation. The key mechanism of hADMSCs-Exo in anti-hepatic fibrosis is the inhibition of the PI3K/AKT/mTOR signaling pathway and the regulation of choline metabolism.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Agnieszka Zok, Mariusz Kaczmarek, Wojciech Pienkowski, Paul Mozdziak, Hanna Piotrowska-Kempisty, Bartosz Kempisty
Summary: Wharton's jelly (WJ) from umbilical cords contains mesenchymal stem cells (MSCs) with immunomodulatory properties and differentiation capacity, which makes them a promising tool for cellular therapies. In this study, WJ-MSCs were cultured for differentiation into osteogenic, chondrogenic, adipogenic, and neurogenic lineages, and differentially expressed genes were identified using RNA analysis. The study provides insights into the transcriptomic changes and identification of novel markers in the differentiation process of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yong Liu, Jing Huang, Rajan Pandey, Pengyuan Liu, Bhavika Therani, Qiongzi Qiu, Sridhar Rao, Aron M. Geurts, Allen W. Cowley Jr, Andrew S. Greene, Mingyu Liang
Summary: This study found that in order to accurately identify differentially expressed genes (DEGs) with various characteristics in single-cell clusters, there need to be at least 2000 cells. However, for the majority of DEGs with extremely small p values or transcript abundance greater than a few hundred transcripts per million in bulk RNA-seq analysis, clusters with as few as 50-100 cells may be sufficient.
Article
Cell Biology
Dovydas Gecys, Ruta Skredeniene, Emilija Gecyte, Arunas Kazlauskas, Ingrida Balnyte, Aiste Jekabsone
Summary: Adipose tissue-derived mesenchymal stem cell extracellular vesicles (ASC-EVs) possess anti-tumor properties, reducing glioblastoma cell proliferation and invasiveness, and can be applied as anticancer therapeutics and medicine carriers.
Article
Cell Biology
Huijiao Lv, Tao Wang, Shangkun Zhai, Zhuocheng Hou, Sirui Chen
Summary: Using transcriptomic analysis, we elucidated the transcriptomic changes during the osteogenic differentiation of chicken BMSCs at different time points. DEGs were mainly enriched in cell proliferation pathways during the early stage of osteogenic differentiation and pathways related to TGF-beta signaling during the middle and late stages. This study provides the first insight into the dynamic transcriptome changes during BMSC differentiation into osteoblasts in chickens.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Wentian Sun, Yidan Song, Kai Xia, Liyuan Yu, Xinqi Huang, Zhihe Zhao, Jun Liu
Summary: A transcriptome-wide m(6)A methylome was built during the osteogenic differentiation of human adipose-derived stem cells (hASCs), revealing the influence of m(6)A on various signaling pathways and hub genes related to bone regeneration. Further epigenetic studies based on these findings could enhance the understanding of the mechanisms underlying hASCs osteogenic differentiation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Sue A. Krause, Gayle Overend, Julian A. T. Dow, David P. Leader
Summary: FlyAtlas 2 is a database and web application for studying gene expression in different tissues of Drosophila melanogaster, with enhancements including a 'Profile' search function and a dedicated feature for exploring gene expression in the larval midgut. The database now includes data from 13 tissues, five sex-specific tissues, and eight larval tissues, with major improvements to the interface.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Mengting Huang, Yixuan Yang, Xingzhao Wen, Weiqiang Xu, Na Lu, Xiao Sun, Jing Tu, Zuhong Lu
Summary: Although single cell RNA sequencing technologies are well developed, acquiring large-scale single cell expression data can still be costly. The study proposes a method of compressing expression profiles from the sample dimension by assigning each cell into multiple pools and demonstrates that expression profiles can be inferred from pool expression data with a overlapping pooling design and compressed sensing strategy. This approach, when combined with plate-based scRNA-seq measurement, maintains superior gene detection sensitivity and individual identity while reducing library costs by half.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Agricultural Engineering
Aleya Ferdausi, Xianmin Chang, Meriel Jones
Summary: This study investigated the differential expression of transcripts related to secondary metabolite biosynthesis in Narcissus basal plate and in vitro callus. Enzymes involved in the initial biosynthetic pathways were upregulated in callus, while genes responsible for regulating the later stage of alkaloid biosynthesis were mainly upregulated in field samples. The findings provide insights into the genes and enzymes involved in secondary metabolism in Narcissus plants.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Multidisciplinary Sciences
Amichay Afriat, Vanessa Zuzarte-Luis, Keren Bahar Halpern, Lisa Buchauer, Sofia Marques, Angelo Ferreira Chora, Aparajita Lahree, Ido Amit, Maria M. Mota, Shalev Itzkovitz
Summary: This study characterized the temporal expression programs of the rodent malaria parasite Plasmodium berghei ANKA and the host hepatocyte in a zonally controlled manner using single-cell RNA sequencing and single-molecule transcript imaging. The study identified differences in parasite gene expression in distinct zones and identified a subpopulation of hepatocytes with reduced levels of Plasmodium transcripts and parasitophorous vacuole breakdown. These findings provide insights into the liver stage of Plasmodium infection.
Article
Biology
Ana Gutierrez-Franco, Franz Ake, Mohamed N. Hassan, Natalie Chaves Cayuela, Loris Mularoni, Mireya Plass
Summary: The critical step in single-cell transcriptomics is sample preparation. Different preservation methods show varying effects on cell quality and expression biases. In this study, methanol fixation is identified as the preferred method for droplet-based single-cell transcriptomics experiments on neural cell populations derived from induced pluripotent stem cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Analytical
Sylwia A. Stopka, Ellen A. Wood, Rikkita Khattar, Beverly J. Agtuca, Walid M. Abdelmoula, Nathalie Y. R. Agar, Gary Stacey, Akos Vertes
Summary: This study utilized mass spectrometry imaging (MSI) to analyze tissue-embedded single cells in high throughput, achieving accurate single-cell sampling through automated image analysis and discovering cellular phenotypes characterized by differing metabolite levels.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Xiaonan Fu, Li Sun, Runze Dong, Jane Y. Chen, Runglawan Silakit, Logan F. Condon, Yiing Lin, Shin Lin, Richard D. Palmiter, Liangcai Gu
Summary: This study introduces a stamping method for fabricating polony gels, reducing the cost and time of barcode sequencing, and developing a single-cell spatial transcriptomic assay. This method offers high resolution and RNA capture efficiency for mapping and analyzing cell-cell communication in tissues.
Article
Cell & Tissue Engineering
Yang Zhou, Bo Zhao, Xin-Liao Zhang, Yi-jun Lu, Shou-Tao Lu, Jian Cheng, Yu Fu, Lin Lin, Ning-Yan Zhang, Pei-Xin Li, Jing Zhang, Jun Zhang
Summary: This study investigated the potential clinical application roles of human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. The combined administration of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration showed additional benefits in promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Multidisciplinary
Zhuo Ye, Moxuan Ji, Kefeng Wu, Jie Yang, An-An Liu, Wei Sun, Dan Ding, Dingbin Liu
Summary: This study introduces a dual-target sequentially activated fluorescence reporting system, iSHERLOCK, for precise identification of atherosclerotic plaques. It aids clinical assessment, diagnosis, and treatment of cardiovascular diseases and overcomes the limitation of precise tissue biopsy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Cardiac & Cardiovascular Systems
Wei Sun, Huayiyang Zou, Yonghong Yong, Boxun Liu, Hao Zhang, Jieyu Lu, Yihui Shen, Peng Li, Tianhua Xu, Xuguan Chen, Anning Du, Ming Jiang, Yang Hua, Yanhui Sheng, Bin Zhou, Chaim Lotan, Xiangqing Kong
Summary: The study demonstrated that percutaneous radiofrequency ablation-based interatrial shunting is a safe and potentially effective therapy for patients with HFpEF, improving clinical outcomes and survival rates.
CIRCULATION-HEART FAILURE
(2022)
Article
Biochemistry & Molecular Biology
Ming Jiang, Jiaming Yang, Huayiyang Zou, Menghuan Li, Wei Sun, Xiangqing Kong
Summary: This study found that in the adult population of the United States, MHR was significantly associated with all-cause and cardiovascular mortality, with participants in the highest MHR tertile having a higher risk compared to those in the lowest tertile.
LIPIDS IN HEALTH AND DISEASE
(2022)
Article
Cell Biology
Ming Qiu, Huanyu Shu, Lu Li, Yejiao Shen, Yunfan Tian, Yue Ji, Wei Sun, Yan Lu, Xiangqing Kong
Summary: This study demonstrates that interleukin 10 (IL-10) has anti-inflammatory effects and can attenuate hypertrophic remodelling caused by overloaded pressure and improve cardiac function. IL-10 levels were found to be decreased in hypertensive patients and angiotensin II (Ang II)-induced hypertensive mice. Treatment with IL-10 reduced aortic thickness, collagen deposition, and fibrosis-associated markers in Ang II-treated mice. IL-10 also inhibited inflammation and oxidative stress in the vascular tissue. Furthermore, IL-10 suppressed proliferation, fibrosis, and inflammation of vascular adventitial fibroblasts. Mechanistically, IL-10 suppressed p38 MAP kinase and NF-kappa B signaling in Ang II-induced vascular fibrosis. Overall, IL-10 shows promise as a potential therapeutic target for limiting aortic remodelling induced by Ang II.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Peripheral Vascular Disease
Chen Cheng, Jin-Yu Sun, Ying Zhou, Qi-Yang Xie, Li-Yuan Wang, Xiang-Qing Kong, Wei Sun
Summary: This study investigated the relationship between waist circumference and hypertension risk in normal-weight/overweight individuals with normal cardiometabolic profiles. The results showed that even in those with normal metabolic profiles, high waist circumference was significantly associated with an increased prevalence of hypertension. Additionally, individuals with high waist circumference and hypertension had elevated all-cause mortality. Therefore, waist circumference should be routinely measured and controlled regardless of metabolic profiles.
JOURNAL OF CLINICAL HYPERTENSION
(2022)
Article
Multidisciplinary Sciences
Peng Li, Boxun Liu, Xiaoguang Wu, Yan Lu, Ming Qiu, Yihui Shen, Yunfan Tian, Chi Liu, Xiru Chen, Chuanxi Yang, Mengqing Deng, Yaqing Wang, Jia Gu, Zhongping Su, Xuguan Chen, Kun Zhao, Yanhui Sheng, Shijiang Zhang, Wei Sun, Xiangqing Kong
Summary: Afferent nerves in perirenal adipose tissue play a key role in maintaining high blood pressure, and their ablation or denervation can reduce blood pressure in hypertensive rats. Calcitonin gene-related peptide is an important substance that suppresses hypertension.
NATURE COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Yue Yuan, Wei Sun, Xiangqing Kong
Summary: Metabolically healthy obesity (MHO) has a negative impact on the development of prehypertension and hypertension, especially in young adults. Additionally, abdominal obesity with a healthy metabolic state is significantly associated with incident hypertension.
DIABETOLOGY & METABOLIC SYNDROME
(2022)
Article
Public, Environmental & Occupational Health
Jin-Yu Sun, Wen-Jun Huang, Yang Hua, Qiang Qu, Chen Cheng, Heng-Li Liu, Xiang-Qing Kong, Yong-Xiang Ma, Wei Sun
Summary: This study investigates the trend in general obesity and abdominal obesity among US adults from 2001 to 2018. The study found that both general obesity and abdominal obesity are significant health burdens among US adults, and the prevalence has been increasing during this time period.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Pharmacology & Pharmacy
Yunfan Tian, Jingyu Sun, Ming Qiu, Yan Lu, Xuesong Qian, Wei Sun, Xiangqing Kong
Summary: The study found a positive association between the TyG index and albuminuria in hypertensive patients. This finding provides important reference for the assessment and treatment of hypertension in patients.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Buqing Ni, Jing Shi, Wei Sun, Yongfeng Shao
EUROPEAN HEART JOURNAL-CASE REPORTS
(2023)
Article
Cell Biology
Yunxi Zhao, Luyang Wang, Mingwei Liu, Anning Du, Ming Qiu, Huanyu Shu, Lu Li, Xiangqing Kong, Wei Sun
Summary: Reactive oxygen species (ROS) have a crucial role in macrophage polarization. However, the harmful effects of reducing ROS through epigenetic influence are often overlooked. In this study, lipopolysaccharide (LPS) was used to stimulate macrophages and increase ROS levels, while N-acetylcysteine (NAC) was used to reduce ROS. Inflammatory factors were used to evaluate macrophage M1 polarization, and it was discovered that reducing ROS led to an increase in the demethylase KDM6A and a decrease in H3K27me3 levels, which in turn increased NOX2 transcription, ROS production, and the production of inflammatory factors. Knocking out KDM6A reduced NOX2 transcription and ROS production, preventing macrophage M1 polarization. In comparison, direct inhibition of KDM6A was found to be more effective in reducing ROS production and inhibiting macrophage M1 polarization.
CELL STRESS & CHAPERONES
(2023)
Article
Cardiac & Cardiovascular Systems
Yang Hua, Jin-Yu Sun, Yu-Xuan Lou, Wei Sun, Xiang-Qing Kong
Summary: This study investigated the association between MLR and mortality and cardiovascular disease mortality. The results showed that individuals with high MLR had a higher risk of death and cardiovascular disease mortality. MLR was a strong independent predictor of mortality and cardiovascular disease mortality in the general population.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuan Ma, Wei Sun, Zhifei Ye, Liuhui Liu, Menghuan Li, Jinhui Shang, Xinyu Xu, Hui Cao, Li Xu, Yongchao Liu, Xiangqing Kong, Guosheng Song, Xiao-Bing Zhang
Summary: This study presents a multiplexed diagnostic tool that has the potential to assess oxidative stress levels in the development of atherosclerosis. The tool incorporates photoacoustic imaging and urinalysis to dynamically report malondialdehyde levels in plaque and urine. By leveraging molecular design and a ratiometric photoacoustic nanoprobe, the tool enables the reflection of intraplaque malondialdehyde and detection of urinary malondialdehyde.
Meeting Abstract
Peripheral Vascular Disease
Yue Yuan, Wei Sun, Xiangqing Kong
JOURNAL OF HYPERTENSION
(2022)
Meeting Abstract
Peripheral Vascular Disease
Yue Yuan, Wei Sun, Xiangqing Kong
JOURNAL OF HYPERTENSION
(2022)
