期刊
3 BIOTECH
卷 11, 期 2, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-020-02623-5
关键词
Porcine parvovirus 7; Loop-mediated isothermal amplification; Visualization; Rapid detection
资金
- Anhui Provincial Primary Research & Developement Plan [202004f06020008, 201904f06020030]
- Ningbo Health Branding Subject Fund
The developed LAMP-based method for detecting PPV7 proved to be simple, rapid, sensitive, and specific, making it suitable for rapid diagnosis in pig farms.
We developed and optimized a loop-mediated isothermal amplification (LAMP)-based method to detect porcine parvovirus 7 (PPV7). After using three pairs of specific primers to amplify PPV7 isothermally at 62 degrees C for 40 min, the amplified product was mixed with SYBR Green I, after which the sample turned green. The method detected PPV7 at concentrations as low as 40 copies/mu L, and the sensitivity was consistent with that of nested polymerase chain reaction (PCR) analysis, which was tenfold higher than that of conventional PCR. No cross-reactivity occurred with porcine parvovirus 1, porcine circovirus type 3, porcine circovirus type 2, porcine pseudorabies virus, porcine epidemic diarrhea virus, or porcine reproductive and respiratory syndrome virus. Simultaneous analysis of 76 clinical samples was performed using LAMP, conventional PCR, and nested PCR. The results showed that our method is simple, rapid, sensitive, and specific for the rapid diagnosis of PPV7 in pig farms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据