Article
Multidisciplinary Sciences
Rui-Kai Zhang, Wei-Yue Sun, Yan-Xia Liu, Emma Y. Zhang, Zhen-Chuan Fan
Summary: This study reveals the mechanism of signal protein export from cilia via the RABL2-ARL3 cascade-mediated outward BBSome transition zone diffusion pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Yan-Xia Liu, Rui-Kai Zhang, Zhen-Chuan Fan
Summary: BBSome is a key component for the export of signaling proteins in cilia. IFT27 binds its partner IFT25 to form the IFT25/27 complex, which is necessary for BBSome reassembly. The interaction between IFT25/27 and the IFT-B subcomplex core IFT-B1 is enhanced by GTP-loading onto IFT27. The presence of IFT25/27 at the ciliary tip facilitates BBSome-dependent PLD export, regardless of the nucleotide state of IFT27.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yan-Xia Liu, Wen -Juan Li, Rui-Kai Zhang, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: Certain ciliary signaling proteins are transported from the tip to the base of cilia via retrograde intraflagellar transport (IFT), facilitated by the BBSome and ARL13. The interaction between ARL13GTP and BBS3GTP at the ciliary tip plays a crucial role in properly coupling the BBSome with signaling cargo. This understanding provides mechanistic insights into the defects seen in hedgehog signaling in humans with ARL13b and BBS3 mutations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Deng-Fu Guo, Ronald A. Merrill, Lan Qian, Ying Hsu, Qihong Zhang, Zhihong Lin, Daniel R. Thedens, Yuriy M. Usachev, Isabella Grumbach, Val C. Sheffield, Stefan Strack, Kamal Rahmouni
Summary: The study reveals that the BBSome protein complex plays a critical role in regulating mitochondrial dynamics and function. The disruption of the BBSome leads to excessive fusion of mitochondria in various cell types, and this morphological change is associated with functional abnormalities, including reduced oxygen consumption rate and altered mitochondrial distribution and calcium handling. Mechanistically, the BBSome modulates the activity of the key regulator protein DRP1 through regulating its phosphorylation and translocation, and rescuing the decrease in DRP1 activity can normalize the defects induced by BBSome deficiency.
MOLECULAR METABOLISM
(2023)
Article
Cell Biology
Xinxing Zhang, Jinzhi Liu, Tong Pan, Alex Ward, Jianfeng Liu, X. Z. Shawn Xu
Summary: BBSome has a cilia-independent function in regulating the stability of LITE-1 protein through DLK-MAPK signaling. BBSome exerts its function by controlling the expression of DLK.
DEVELOPMENTAL CELL
(2022)
Review
Peripheral Vascular Disease
Yuying Zhao, Kamal Rahmouni
Summary: The BBSome is a protein complex associated with Bardet-Biedl syndrome (BBS) and has been linked to various cardiovascular conditions. It was initially believed to be involved in cilia function, but further studies have shown its role in multiple cellular processes, including receptor localization and gene expression. Animal models have helped uncover the importance of the BBSome in cardiovascular regulation, such as blood pressure control, renal function, vascular reactivity, and cardiac development. The BBSome also plays a critical role in key systems involved in cardiovascular control, including the renin-angiotensin system.
Article
Biochemistry & Molecular Biology
Francesco Chiuso, Rossella delle Donne, Giuliana Giamundo, Laura Rinaldi, Domenica Borzacchiello, Federica Moraca, Daniela Intartaglia, Rosa Iannucci, Emanuela Senatore, Luca Lignitto, Corrado Garbi, Paolo Conflitti, Bruno Catalanotti, Ivan Conte, Antonio Feliciello
Summary: Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal degeneration, obesity, renal abnormalities, postaxial polydactyly, and developmental defects. The E3 ubiquitin ligase PJA2 has been identified as a regulator of the BBSome, an octameric complex that controls ciliary trafficking. Ubiquitylation of BBS1 by PJA2 stabilizes the BBSome and promotes its binding to BBS3, leading to proper ciliary membrane targeting. Disruption of PJA2 or expression of a ubiquitylation-defective BBS1 mutant affects GPCR trafficking and gene transcription, recapitulating the BBS phenotype in a medaka fish model.
Review
Biochemistry & Molecular Biology
Xiumin Yan, Yidong Shen
Summary: Primary cilia are important sensory organelles in cells, with intraflagellar transport essential for their assembly, maintenance, and function. Recent studies have highlighted the critical regulatory roles of three ciliary Rab-like small GTPases in ciliary BBSome transport.
Article
Biochemistry & Molecular Biology
Shichao Duan, Hao Li, Yirong Zhang, Suming Yang, Yawen Chen, Benhua Qiu, Cheng Huang, Juan Wang, Jinsong Li, Xueliang Zhu, Xiumin Yan
Summary: This study reveals the molecular switch function of small GTPase Rabl2 in the passage through the ciliary barrier transition zone, where GTP hydrolysis regulates the outward passage of BBSome and its cargos with retrograde IFT machinery crucial for proper ciliary signaling.
Article
Cell Biology
Toneisha Stubbs, James I. Bingman, Jason Besse, Kirk Mykytyn
Summary: Primary cilia are important for neuronal functions and their dysfunction is linked to ciliopathies. Bardet-Biedl syndrome proteins play a crucial role in trafficking G protein-coupled receptors (GPCRs) in neuronal cilia, and their absence leads to abnormal localization of signaling proteins in the brain.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Neurosciences
Toneisha Stubbs, Andrew Koemeter-Cox, James I. Bingman, Fangli Zhao, Anuradha Kalyanasundaram, Leslie A. Rowland, Muthu Periasamy, Calvin S. Carter, Val C. Sheffield, Candice C. Askwith, Kirk Mykytyn
Summary: A rod-shaped appendage called a primary cilium projects from most central neurons in the mammalian brain. Cilia are important for neuronal signaling and their dysfunction is associated with various neuropathologies. This study demonstrates that disrupting the localization of a specific ciliary GPCR, called dopamine receptor 1 (D-1), in neurons leads to obesity and reduced locomotor activity in male and female mice. Loss of a BBS protein or cilia on D-1-expressing neurons also reduces D-1-mediated signaling. These findings highlight the importance of neuronal cilia for proper GPCR signaling and shed light on the role of cilia in regulating weight and locomotor activity.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Chao Xie, Julien C. Habif, Kirill Ukhanov, Cedric R. Uytingco, Lian Zhang, Robert J. Campbell, Jeffrey R. Martens
Summary: Ciliopathies are a class of genetic diseases that cause dysfunction in cilia in multiple organ systems, including the olfactory system. This study investigates the mechanism behind the reduction of olfactory cilia in ciliopathy Bardet-Biedl syndrome (BBS) and finds that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and F-actin play important roles in cilia shortening. The researchers successfully restored cilia length and rescued odor detection and perception in BBS using a gene therapeutic approach. These findings provide valuable insights for the treatment of ciliopathies.
Review
Urology & Nephrology
Ailis L. Moran, Laura Louzao-Martinez, Dominic P. Norris, Dorien J. M. Peters, Oliver E. Blacque
Summary: Primary cilia serve as cell surface antennae, coordinating cellular responses to sensory inputs and signaling molecules that regulate development and homeostasis. The compartmentalized structure of cilia, along with active transport pathways and gating mechanisms, plays a crucial role in maintaining its unique molecular composition and signaling environment. Understanding the major compartmentalizing pathways at the cilium has provided insights into ciliary disease and associated renal disorders.
NATURE REVIEWS NEPHROLOGY
(2023)
Article
Cell Biology
Longhui Zeng, Ivan Palaia, Andela Saric, Xiaolei Su
Summary: Phospholipase C gamma 1 (PLC gamma 1) promotes phase separation of LAT in the TCR pathway, directly cross-linking LAT through its two SH2 domains. PLC gamma 1 also protects LAT from dephosphorylation by CD45, and enhances LAT-dependent ERK activation and SLP76 phosphorylation. A nonmonotonic effect of PLC gamma 1 on LAT clustering was discovered, and computer simulations showed how cluster size is regulated by protein compositions. These results define a critical role of PLC gamma 1 in promoting phase separation of the LAT complex and TCR signal transduction.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Cell Biology
Staci E. Engle, Ruchi Bansal, Patrick J. Antonellis, Nicolas F. Berbari
Summary: Ciliopathies are rare genetic disorders associated with pediatric obesity, and the mechanisms linking cilia dysfunction to obesity are complex. In addition to syndromic forms of obesity, mutations in cilia signaling genes can also lead to morbid obesity. Cilia play critical roles in energy homeostasis, potentially impacting obesity development and maintenance in both central and peripheral tissues.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Environmental
Yunxue Li, Sitong Liu, Zhiyong Ji, Jun Sun, Xianhua Liu
Summary: This study systematically investigated the sorption behavior of two typical microplastics (PVC and PE) to Zn(II) and their combined toxic effects on Chlorella vulgaris. The results showed that different microplastics exhibited different adsorption capacities for Zn(II) and the combined exposure to Zn(II) and microplastics had distinct effects on cell growth and oxidative stress.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Yuqiu Wei, Dongsheng Ding, Ting Gu, Yong Xu, Xuemei Sun, Keming Qu, Jun Sun, Zhengguo Cui
Summary: Data on the independent or combined effects of ocean acidification and warming on coastal eutrophication and organic pollution are limited. Acidification favors eutrophication and organic pollution, while warming inhibits these two variables. However, the interaction between acidification and warming in the future may exacerbate organic pollution but mitigate eutrophication.
MARINE POLLUTION BULLETIN
(2023)
Article
Biology
Satheeswaran Thangaraj, Jun Sun
Summary: Physiological experiments on Heterosigma akashiwo provide insights into the metabolic changes of algal blooms under future warming and acidification of the ocean. Despite the expected increase in harmful algal species due to ocean warming and acidification, the population of Heterosigma akashiwo has decreased. However, the physical and metabolic modifications of this species in terms of C:N:P ratio and macromolecule accumulation are still unknown.
COMMUNICATIONS BIOLOGY
(2023)
Article
Oceanography
Congcong Guo, Guicheng Zhang, Shan Jian, Wei Ma, Jun Sun
Summary: The quantification of transparent exopolymer particles (TEP) by colorimetric method is prone to large errors and low repeatability, mainly due to undefined and unassessed steps in the original method. It is uncertain whether the 80% sulfuric acid solution used as the extraction solution for TEP determination is prepared based on volume ratio or mass ratio. In addition, changes in the solubility of Gum Xanthan (GX) from the market rendered the original protocol ineffective, and the grinding of GX stock solution was replaced with shaking using a rotating shaker to prevent excessive dissolution. Different preparation techniques resulted in varied concentrations of 80% H2SO4. The shaking duration during the preparation of standard solution significantly affected the calibration curve slope, leading to different correction results for TEP.
ACTA OCEANOLOGICA SINICA
(2023)
Article
Environmental Sciences
Xudong Cui, Liying Peng, Yu Guo, Guicheng Zhang, Haijiao Liu, Yujian Wen, Guodong Zhang, Jun Sun
Summary: This paper investigates the distribution, sources, and deposition fluxes of aerosol nutrients in the Bohai Bay and their impacts on the marine ecosystem. The results show that the concentrations of typical aerosol nutrients are generally higher in autumn than in summer, with high values mainly occurring in the central region. The potential sources of pollution are mainly concentrated in Shandong and Mongolia, and include agriculture, dust, and industry.
ENVIRONMENTAL POLLUTION
(2023)
Article
Cell Biology
Yan-Xia Liu, Rui-Kai Zhang, Zhen-Chuan Fan
Summary: BBSome is a key component for the export of signaling proteins in cilia. IFT27 binds its partner IFT25 to form the IFT25/27 complex, which is necessary for BBSome reassembly. The interaction between IFT25/27 and the IFT-B subcomplex core IFT-B1 is enhanced by GTP-loading onto IFT27. The presence of IFT25/27 at the ciliary tip facilitates BBSome-dependent PLD export, regardless of the nucleotide state of IFT27.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yan-Xia Liu, Wen -Juan Li, Rui-Kai Zhang, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: Certain ciliary signaling proteins are transported from the tip to the base of cilia via retrograde intraflagellar transport (IFT), facilitated by the BBSome and ARL13. The interaction between ARL13GTP and BBS3GTP at the ciliary tip plays a crucial role in properly coupling the BBSome with signaling cargo. This understanding provides mechanistic insights into the defects seen in hedgehog signaling in humans with ARL13b and BBS3 mutations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Environmental
Yang Liu, Xiaofang Liu, Yi Long, Yujian Wen, Chao Ma, Jun Sun
Summary: This study investigates the molecular composition and component characteristics of marine dissolved organic matter (DOM) in the eastern Indian Ocean. It reveals the presence of thousands of individual DOM formulas, including recalcitrant compounds and stable components. The study also uncovers a shift in the composition of DOM with depth, indicating the aging of the water column.
Article
Environmental Sciences
Wenzhuo Yan, Zhuo Chen, Linlin Zhang, Feng Wang, Guicheng Zhang, Jun Sun
Summary: This study used the Utermohl method and carbon volume conversion to investigate the composition and distribution of phytoplankton in the western Tropical Pacific Ocean. Four primary groups of phytoplankton were identified: dinoflagellates (181 species), diatoms (73 species), cyanobacteria (4 species), and chrysophyceae (2 species). Clustering analysis revealed that phytoplankton could be classified into four groups based on their composition, which were closely related to ocean currents. The study also found that diatoms were abundant in areas influenced by current-seamount interaction, while areas with little influence from ocean currents were dominated by Trichodesmium. The majority of phytoplankton had an equivalent spherical diameter (ESD) of 2-12 μm, with some exceeding 25 μm. Nanophytoplankton (ESD = 2-20 μm) dominated cell abundance, but microphytoplankton (ESD = 20-200 μm) contributed significantly to carbon biomass (792.295 mg m(-3)). This study provides valuable insights into the distribution and composition of phytoplankton in the western Tropical Pacific Ocean and their relationship with ocean currents, as well as fundamental information regarding cell size and carbon biomass within the region.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Environmental Sciences
Ziming Wang, Dai Jia, Shuai Song, Jun Sun
Summary: Given the threat to water quality from human activities, it is necessary to identify and quantify potential pollution sources for water pollution control. Multivariate methods were used to assess water quality in the Yuqiao Reservoir and surrounding rivers, and identified seven main pollution sources including cities, rural districts, industries, weather, fertilizers, upstream areas, and vehicles. The results showed that upstream and urban districts were the major contributors to pollution. The study also compared positive matrix factorization (PMF) and absolute principal component scores and multiple linear regression (APCS-MLR) modeling, with APCS-MLR performing better.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Rui-Kai Zhang, Wei-Yue Sun, Yan-Xia Liu, Emma Y. Zhang, Zhen-Chuan Fan
Summary: This study reveals the mechanism of signal protein export from cilia via the RABL2-ARL3 cascade-mediated outward BBSome transition zone diffusion pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Chan Zhang, Rui-Kai Zhang, Ying Feng, Sheng-Nan Sun, Zhen-Chuan Fan
Summary: In this study, a fish-derived antimicrobial peptide (AMP), CATH_BRALE, was expressed in Chlamydomonas reinhardtii as a recombinant peptide with stable expression and broad-spectrum antibacterial activity. It showed strong thermostability and pH stability, and did not exhibit toxicity on different cell lines. The results demonstrate the potential of using C. reinhardtii as a host for producing biologically active CATH_BRALE.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hui Wang, Ying Feng, Hadiatullah Hadiatullah, Zhen-Chuan Fan
Summary: Researchers expressed antimicrobial peptides Mytichitin-CB and Hispidalin in Chlamydomonas reinhardtii and created a chimeric peptide called MCH. MCH demonstrated high efficiency in inhibiting bacterial growth and displayed strong resistance to temperature, pH, and proteases, while showing low toxicity towards erythrocytes and human kidney cells.
PROCESS BIOCHEMISTRY
(2023)
Article
Environmental Sciences
Yiyan Guo, Chao Wu, Zhi Wang, Yifeng Shi, Jun Sun
Summary: This study provides preliminary insights into the distribution patterns of bacterial communities and metal resistance genes in the coastal sediments of Bohai Bay, laying the groundwork for understanding microbial community adaptations and supporting ecological restoration efforts in multi-metal polluted environments.
ENVIRONMENTAL POLLUTION
(2023)
Article
Geosciences, Multidisciplinary
Zhibo Shao, Yangchun Xu, Hua Wang, Weicheng Luo, Lice Wang, Yuhong Huang, Nona Sheila R. Agawin, Ayaz Ahmed, Mar Benavides, Mikkel Bentzon-Tilia, Ilana Berman-Frank, Hugo Berthelot, Isabelle C. Biegala, Mariana B. Bif, Antonio Bode, Sophie Bonnet, Deborah A. Bronk, Mark V. Brown, Lisa Campbell, Douglas G. Capone, Edward J. Carpenter, Nicolas Cassar, Bonnie X. Chang, Dreux Chappell, Yuh-ling Lee Chen, Matthew J. Church, Francisco M. Cornejo-Castillo, Amalia Maria Sacilotto Detoni, Scott C. Doney, Cecile Dupouy, Marta Estrada, Camila Fernandez, Bieito Fernandez-Castro, Debany Fonseca-Batista, Rachel A. Foster, Ken Furuya, Nicole Garcia, Kanji Goto, Jesus Gago, Mary R. Gradoville, M. Robert Hamersley, Britt A. Henke, Cora Horstmann, Amal Jayakumar, Zhibing Jiang, Shuh-Ji Kao, David M. Karl, Leila R. Kittu, Angela N. Knapp, Sanjeev Kumar, Julie LaRoche, Hongbin Liu, Jiaxing Liu, Caroline Lory, Carolin R. Loscher, Emilio Maranon, Lauren F. Messer, Matthew M. Mills, Wiebke Mohr, Pia H. Moisander, Claire Mahaffey, Robert Moore, Beatriz Mourino-Carballido, Margaret R. Mulholland, Shin-ichiro Nakaoka, Joseph A. Needoba, Eric J. Raes, Eyal Rahav, Teodoro Ramirez-Cardenas, Christian Furbo Reeder, Lasse Riemann, Virginie Riou, Julie C. Robidart, Vedula V. S. S. Sarma, Takuya Sato, Himanshu Saxena, Corday Selden, Justin R. Seymour, Dalin Shi, Takuhei Shiozaki, Arvind Singh, Rachel E. Sipler, Jun Sun, Koji Suzuki, Kazutaka Takahashi, Yehui Tan, Weiyi Tang, Jean-Eric Tremblay, Kendra Turk-Kubo, Zuozhu Wen, Angelicque E. White, Samuel T. Wilson, Takashi Yoshida, Jonathan P. Zehr, Run Zhang, Yao Zhang, Ya-Wei Luo
Summary: This study presents an updated version (version 2) of the global oceanic diazotroph database, which significantly increases the number of in situ diazotrophic measurements and provides more detailed insights into nitrogen fixation in the global ocean. Version 2 yields significantly higher estimates of nitrogen fixation rates compared to version 1, particularly in the South Pacific and North Atlantic regions. Furthermore, additional data sheets for non-cyanobacterial diazotrophs and cell-specific N2 fixation rates have been included in version 2, enhancing the database's comprehensiveness.
EARTH SYSTEM SCIENCE DATA
(2023)