期刊
PLANT GENOME
卷 14, 期 1, 页码 -出版社
WILEY
DOI: 10.1002/tpg2.20084
关键词
-
资金
- Earmarked Fund for China Agriculture Research System [CARS-27]
This study identified 13 QTL, including three major ones, on chromosome 03, 08, and 16, influencing the shelf life of apple fruits. A candidate gene encoding pectin acetylesterase negatively affected fruit shelf life, with a deletion in the coding sequence disrupting its function and a SNP in the promoter region reducing its transcription activity.
Room-temperature shelf life is a key factor in fresh market apple (Malus domestica Borkh.) quality and commercial value. To investigate the genetic and molecular mechanism underlying apple shelf life, quantitative trait loci (QTL) were identified using bulked segregant analysis via sequencing (BSA-seq). Ethylene emission, flesh firmness, or crispness of apple fruit from 1,273 F-1 plants of M. asiatica Nakai 'Zisai Pearl' x M. domestica 'Golden Delicious' were phenotyped prior to and during 6 wk of room-temperature storage. Segregation of ethylene emission and the flesh firmness or crispness traits was detected in the population. Thirteen QTL, including three major ones, were identified on chromosome 03, 08, and 16. A candidate gene encoding pectin acetylesterase, MdPAE10, from the QTL Z16.1 negatively affected fruit shelf life. A 379-bp deletion in the coding sequence of MdPAE10 disrupted its function. A single nucleotide polymorphism (SNP) in the MdPAE10 promoter region reduced its transcription activity. These findings provided insight into the genetic control of fruit shelf life and can be potentially used in apple marker-assisted selection.
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