Article
Multidisciplinary Sciences
Monira Obaid, S. M. Nashir Udden, Prasanna Alluri, Subhrangsu S. Mandal
Summary: The study demonstrates that during inflammation, HOTAIR plays a key role in regulating Glut1 expression and glucose uptake by activating NF-kB, thereby controlling metabolic programming in immune cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Benjamin R. E. Harris, Ye Zhang, Jianxin Tao, Renhui Shen, Xiaoqian Zhao, Margot P. Cleary, Tong Wang, Da-Qing Yang
Summary: Enhanced glucose uptake in cancer cells is closely correlated with poor prognosis, and the protein kinase ATM plays a key role in insulin-mediated glucose uptake. Inhibition of ATM by KU-55933 inhibits glucose uptake and glycolysis, induces apoptosis, and inhibits motility of cancer cells with overactivated Akt, showing potential as a therapeutic agent against cancer.
Article
Pharmacology & Pharmacy
Qun Lou, Meichen Zhang, Yanmei Yang, Yanhui Gao
Summary: Long term low dose exposure to arsenic can lead to cell proliferation and malignant transformation. In this study, it was found that low dose exposure to arsenic trioxide increased glucose uptake and promoted cell viability and DNA synthesis. The activation of AKT induced by arsenic trioxide upregulated the expression of GLUT1 on the plasma membrane, enhancing glucose uptake and cell proliferation.
Article
Biochemistry & Molecular Biology
Lauren L. Daniele, John Y. S. Han, Ivy S. Samuels, Ravikiran Komirisetty, Nikhil Mehta, Jessica L. McCord, Minzhong Yu, Yekai Wang, Kathleen Boesze-Battaglia, Brent A. Bell, Jianhai Du, Neal S. Peachey, Nancy J. Philp
Summary: Glucose supply is crucial for the normal functioning and survival of retinal cells. This study demonstrates the essential role of glucose in maintaining and sustaining rod photoreceptor outer segments (OS), with minimal impact on cone photoreceptor cells.
Article
Biochemistry & Molecular Biology
Adwaid Manu Krishna Chandran, Daniela Coltrini, Mirella Belleri, Sara Rezzola, Elena Gambicorti, Davide Romano, Francesco Morescalchi, Stefano Calza, Francesco Semeraro, Marco Presta
Summary: Idiopathic epiretinal membranes (ERMs) are fibrocellular membranes consisting of extracellular matrix proteins and epiretinal cells. Muller cells may play a key role in the pathogenesis of iERM, with different molecular profiles and clinical features observed in different patient clusters.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yingying Yue, Chang Zhang, Xiaoyun Zhao, Sasa Liu, Xiaoting Lv, Shitian Zhang, Jianming Yang, Liming Chen, Hongquan Duan, Youyi Zhang, Zhi Yao, Wenyan Niu
Summary: Research has shown that Tiam1 protein is part of an AMPK-Tiam1-Rac1 signaling pathway that mediates contraction-stimulated glucose uptake in skeletal muscle cells and tissue.
Article
Biochemistry & Molecular Biology
Kathryn E. Hamilton, Miranda F. Bouwer, Larry L. Louters, Brendan D. Looyenga
Summary: The study suggests that fluorescent analogs 2-NBDG and 6-NBDG may enter mammalian cells through mechanisms independent of transporters, casting doubt on their utility as accurate proxies for glucose transport.
Article
Pharmacology & Pharmacy
Silvia M. Torres, Francisca P. Carmo, Luis C. Monteiro, Claudia Silva, Nelson Andrade, Fatima Martel
Summary: Phenolic acids have been recognized as effective agents for chemoprevention and chemotherapy. This study aimed to investigate the impact of phenolic acids on glucose and glutamine uptake in breast cancer cells (MCF-7) and pancreatic cancer cells (AsPC-1). The results showed that certain phenolic acids affected the uptake of both 3H-glutamine and 3H-deoxy-D-glucose (3H-DG).
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Oncology
Junwei Zou, Zhenhan Li, Jiaheng Xie, Zhaoying Wu, Yujin Huang, Hao Xie, Huiqiu Xu, Yong Huang, Hailang Zhou
Summary: Colon cancer is a common malignant tumor with limited effective treatment. Recent studies have shown a potential association between ferroptosis and autophagy in colon cancer. In this study, we investigated the clinical significance and prognostic value of the gene SLC2A1 in colon cancer, and found a correlation between its methylation and immune suppression in tumor tissue. Our findings suggest that SLC2A1 may serve as a new therapeutic target for colon cancer.
JOURNAL OF ONCOLOGY
(2022)
Article
Cell Biology
Xiaoguang Liu, Litong Nie, Yilei Zhang, Yuelong Yan, Chao Wang, Medina Colic, Kellen Olszewski, Amber Horbath, Xiong Chen, Guang Lei, Chao Mao, Shiqi Wu, Li Zhuang, Masha V. Poyurovsky, M. James You, Traver Hart, Daniel D. Billadeau, Junjie Chen, Boyi Gan
Summary: Liu, Nie et al. discovered that glucose starvation leads to cell death mediated by SLC7A11, which suppresses the activity of the ferroptosis pathway. They further found that glucose starvation in SLC7A11(high) cells causes abnormal formation of disulfide bonds in actin cytoskeleton proteins, resulting in a newly characterized form of cell death called disulfidptosis. They also revealed that inhibiting aberrant disulfide bonds in actin cytoskeleton suppresses disulfidptosis, while activating the ferroptosis pathway promotes disulfidptosis. Additionally, glucose transporter inhibitors induce disulfidptosis and inhibit the growth of SLC7A11(high) tumors.
NATURE CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shawna L. L. McMillin, Parker L. L. Evans, William M. M. Taylor, Luke A. A. Weyrauch, Tyler J. J. Sermersheim, Steven S. S. Welc, Monique R. R. Heitmeier, Richard C. C. Hresko, Paul W. W. Hruz, Francoise Koumanov, Geoffrey D. D. Holman, E. Dale Abel, Carol A. A. Witczak
Summary: The study found that GLUT1 does not mediate basal muscle glucose uptake and suggests the existence of a novel glucose transport mechanism for overload-stimulated glucose uptake.
Article
Multidisciplinary Sciences
Giulia Maria Piperno, Francesca Simoncello, Oriana Romano, Simone Vodret, Yuichi Yanagihashi, Regine Dress, Charles-Antoine Dutertre, Mattia Bugatti, Pierre Bourdeley, Annalisa Del Prete, Tiziana Schioppa, Emilia Maria Cristina Mazza, Licio Collavin, Serena Zacchigna, Renato Ostuni, Pierre Guermonprez, William Vermi, Florent Ginhoux, Silvio Bicciato, Shigekatzu Nagata, Nicoletta Caronni, Federica Benvenuti
Summary: Loss of TIM4 expression in lung tumor-associated cDC1 leads to inefficient uptake of cell-associated antigens and reduced activation of CD8+ T cells in advanced lung tumors. This contributes to immune surveillance and anti-tumor immune responses.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Camila Gherardelli, Pedro Cisternas, Joel Gutierrez, Milka Martinez, Nibaldo C. Inestrosa
Summary: Cerebral glucose hypometabolism is a common feature in many neurodegenerative diseases, and it has been shown that metabolic disturbances precede clinical symptoms. Andrographolide can enhance neuronal glucose uptake and metabolism, dependent on the activity of AMP-activated protein kinase.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Ophthalmology
Xiaorui Wang, Xinxing Zhu, Guangqian Huang, Lili Wu, Zhiyong Meng, Yuyu Wu
Summary: This study investigated the relationship between endoplasmic reticulum stress and Muller glial cells during retinal ischemia and hypoxia. The downregulation of PERK expression had an anti-apoptotic effect on OGD-conditioned rMC-1 cells, suggesting a possible mechanism of MG cell apoptosis during retinal ischemic injury.
Article
Neurosciences
Osmar Vieira Ramires Junior, Josiane Silva Silveira, Tiago Marcon dos Santos, Fernanda Silva Ferreira, Adriana Fernanda K. Vizuete, Carlos Alberto Goncalves, Angela T. S. Wyse
Summary: This study verified the effects of Homocysteine (Hcy) on neurochemical parameters and signaling pathway in hippocampal slices, and investigated the neuroprotective effects of ibuprofen and rivastigmine. Hcy increased cellular and tissue damage, while ibuprofen reduced its damaging effects. The combination treatment of rivastigmine and ibuprofen attenuated Hcy-induced damage by regulating the signaling pathway.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Virginia Actis Dato, Aleyda Benitez-Amaro, David de Gonzalo-Calvo, Maximiliano Vazquez, Gustavo Bonacci, Vicenta Llorente-Cortes, Gustavo Alberto Chiabrando
Article
Ophthalmology
Marilyn A. Marquez, Claudio P. Juarez, Maria C. Sanchez, Jose D. Luna
Summary: This case report describes a unique presentation in a patient with NF1, rubeosis iridis, high IOP, and macular hemorrhages from multiple corkscrew retinal vessels in a well-perfused retina. Treatment with anti-angiogenics led to a good visual outcome.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Virginia Actis Dato, Gustavo Alberto Chiabrando
Summary: Studies have shown that α-2 Macroglobulin activated protein enhances insulin-induced glucose uptake in myocardium by promoting GLUT4 recycling through PI3K/Akt and MAPK/ERK signaling pathways and reduces intracellular cholesteryl ester accumulation. This antagonist action on LRP1 counteracts the detrimental effects of aggregated LDL in cardiomyocytes, potentially offering therapeutic implications for cardiovascular diseases associated with hypercholesterolemia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Paula Subirada, Maria C. Paz, Maria V. Vaglienti, Jose D. Luna, Pablo F. Barcelona, Maria C. Sanchez
Summary: Retinopathies are a group of diseases that affect the neurosensory tissue of the eye, leading to neurodegeneration and changes in vascular function and structure. By using animal models and image processing, we can measure retinal vascular parameters such as density, diameter, tortuosity, and neovascular areas. These tools are highly helpful in evaluating and quantifying vascular alterations in retinopathies.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Paula V. Subirada, Maria V. Vaglienti, Mariana B. Joray, Maria C. Paz, Pablo F. Barcelona, Maria C. Sanchez
Summary: This study investigated the changes in autophagy flux during hypoxia and hypoxia-reoxygenation, as well as the effects of Rapamycin and Resveratrol on the gliotic and pro-angiogenic response of Muller glial cells (MGCs) under hypoxic conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Virginia Actis Dato, Aleyda Benitez-Amaro, Eduardo Garcia, Lene Claudi, Maria Teresa LaChica Lhoest, Antoni Iborra, Jose Maria Guerra, Joan Carles Escola-Gil, Valerie Samouillan, Carlos Enrich, Gustavo Chiabrando, Vicenta Llorente-Cortes
Summary: The study found that targeting the P3 sequence on LRP1 through anti-P3 antibodies can specifically reduce CE accumulation in the heart and restore InsR and GLUT4 levels in the hearts of rabbits fed with a high-fat diet.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Ricardo A. Albertini, Juan C. Nicolas, Virginia Actis Dato, Dario G. Ferrer, Maria E. Tinti, Raul H. Capra, Gustavo A. Chiabrando
Summary: This study found that the expression of LRP1 on the surface of circulating monocytes is reduced in asymptomatic individuals with atherosclerosis, indicating its association with the development of the disease.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Immunology
Maria Florencia Soler, Rocio del Carmen Bravo-Miana, Dana Maria Negretti-Borga, Paula Subirada, Vanina Alejandra Alamino, Maria Cecilia Sanchez, Ana Carolina Donadio, Claudia Gabriela Pellizas, Maria del Mar Montesinos
Summary: T3-conditioned dendritic cell vaccine shows effective anti-tumor effects in a murine model of colon carcinoma, enhancing immune response by increasing CD86 expression, promoting the production of inflammatory factors, and inducing specific cytotoxic T lymphocytes. T3 also promotes the maturation and activation of human dendritic cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Paula Virginia Subirada, Albana Tovo, Maria Victoria Vaglienti, Jose Domingo Luna Pinto, Horacio Uri Saragovi, Maria Cecilia Sanchez, Agustin Anastasia, Pablo Federico Barcelona
Summary: Choroidal neovascularization (CNV) is a pathological angiogenesis of the choroidal plexus of the retina and is a key feature in the wet form of age-related macular degeneration. Mononuclear phagocytic cells (MPCs) are recruited and activated through p75 neurotrophin receptor (p75(NTR)) in a mouse model of laser-induced CNV, contributing to CNV pathology. Knockout of p75(NTR) or treatment with its antagonist reduces MPC recruitment, glial activation, CNV area, and preserves retinal function in mice with CNV.
Article
Cell Biology
Maria V. Vaglienti, Paula V. Subirada, Mariana B. Joray, Gustavo Bonacci, Maria C. Sanchez
Summary: Inflammation, oxidative stress, and nitrosative stress are involved in the pathogenesis of proliferative retinopathies. Nitro-oleic acid (NO2-OA) shows a protective effect on oxidative damage, gliosis, and the pro-angiogenic response in Muller glial cells.
Article
Chemistry, Medicinal
Valerie Samouillan, Eduardo Garcia, Aleyda Benitez-Amaro, Maria Teresa La Chica Lhoest, Jany Dandurand, Virginia Actis Dato, Jose Maria Guerra, Joan Carles Escola-Gil, Gustavo Chiabrando, Carlos Enrich, Vicenta Llorente-Cortes
Summary: The accumulation of lipids in cardiomyocytes leads to cardiac dysfunction. Blocking cardiomyocyte cholesteryl ester (CE) loading specifically with antibodies against the P3 sequence of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 antibodies on biophysical alterations was analyzed.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Virginia Actis Dato, Maria C. Paz, Federico E. Rey, Maria C. Sanchez, Vicenta Llorente-Cortes, Gustavo A. Chiabrando, Danilo G. Ceschin
Summary: Cardiovascular disease is associated with high levels of atherogenic lipoproteins, which result in lipid accumulation and alterations in gene expression profiles essential for cardiac functionality. These changes can lead to impaired insulin response, disrupted lipid metabolism, altered cardiac structure, and increased susceptibility to cardiovascular events.
SCIENTIFIC REPORTS
(2023)
Review
Neurosciences
Maria C. Sanchez, Gustavo A. Chiabrando
Summary: Muller glial cells (MGCs) play an important role in retinal homeostasis and the development of proliferative retinopathies, while low-density lipoprotein receptor-related protein 1 (LRP1) has been identified as a key player in these processes. Further research in this area could lead to novel therapeutic approaches.