期刊
CELL DEATH & DISEASE
卷 12, 期 2, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41419-021-03471-8
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资金
- grant from Canceropole Grand Ouest-Region Pays de la Loire (CONCERTO)
- Ligue Contre le Cancer
- Comite Grand Ouest de la Ligue Contre le Cancer
This study revealed that Bcl2 is mainly localized in the ER, and is translocated to MAM and mitochondria during apoptosis, with its interaction with TOM20 playing a significant role. The findings suggest that the Bcl2-TOM20 interaction is proapoptotic and contributes to the control of apoptosis.
In this work, we have explored the subcellular localization of Bcl2, a major antiapoptotic protein. In U251 glioma cells, we found that Bcl2 is localized mainly in the ER and is translocated to MAM and mitochondria upon induction of apoptosis; this mitochondrial transfer was not restricted to the demonstrator cell line, even if cell-specific modulations exist. We found that the Bcl2/mitochondria interaction is controlled by TOM20, a protein that belongs to the protein import machinery of the mitochondrial outer membrane. The expression of a small domain of interaction of TOM20 with Bcl2 potentiates its anti-apoptotic properties, which suggests that the Bcl2-TOM20 interaction is proapoptotic. The role of MAM and TOM20 in Bcl2 apoptotic mitochondrial localization and function has been confirmed in a yeast model in which the ER-mitochondria encounter structure (ERMES) complex (required for MAM stability in yeast) has been disrupted. Bcl2-TOM20 interaction is thus an additional player in the control of apoptosis.
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