Article
Engineering, Biomedical
Yong Hun Jung, Dong-Hee Choi, Kyungwon Park, Sat-Byol Lee, Jonghun Kim, Hyunho Kim, Hyun-Woo Jeong, Ji Hun Yang, Jin-A Kim, Seok Chung, Byung Soh Min
Summary: This study demonstrates a scalable organoid production platform that can produce functionally complex and uniform organoids. Hydro-organoids produced on the new platform show better consistency in drug screening and have the potential to be used in high-throughput assays and predicting clinical outcomes.
Review
Genetics & Heredity
Toshikatsu Matsui, Tadahiro Shinozawa
Summary: Organoids, three-dimensional structures fabricated in vitro, mimic complex microenvironments and physiological functions, bridging the gap between animals and humans in research. In toxicology and drug development, human organoids offer new possibilities by overcoming limitations of current models.
FRONTIERS IN GENETICS
(2021)
Review
Engineering, Biomedical
Zahra Heydari, Farideh Moeinvaziri, Tarun Agarwal, Paria Pooyan, Anastasia Shpichka, Tapas K. Maiti, Peter Timashev, Hossein Baharvand, Massoud Vosough
Summary: The limitations of monolayer culture have prompted scientists to explore new models that can accurately replicate the architecture and function of human organs. Recent advancements in protocol improvement have led to the development of three-dimensional organ-like structures known as 'organoids', which can mimic the characteristics of real organs. These organoid-based 3D models offer novel opportunities for drug screening, toxicology research, and molecular pathophysiology studies.
BIO-DESIGN AND MANUFACTURING
(2021)
Article
Medicine, General & Internal
Shaojun Shi, Henk P. Roest, Thierry P. P. van den Bosch, Marcel J. C. Bijvelds, Markus U. Boehnert, Jeroen de Jonge, Sven O. Dekker, Antoine A. F. de Vries, Hugo R. de Jonge, Monique M. A. Verstegen, Luc J. W. van der Laan
Summary: This study successfully replicated ischemic cholangiopathy in vitro using human intrahepatic cholangiocyte organoids stressed by ischemia and subsequent re-oxygenation. The model exhibited similar changes in cell proliferation, epithelial-mesenchymal transition, and cell death processes as observed in patients. The research also identified alpha-1 antitrypsin as a potent inhibitor of ischemia-induced apoptosis and necroptosis.
Review
Pharmacology & Pharmacy
Yen-Dun Tony Tzeng, Jui-Hu Hsiao, Ling-Ming Tseng, Ming-Feng Hou, Chia-Jung Li
Summary: Breast cancer is a common and heterogeneous malignancy in women worldwide. Traditional research methods have limitations, but three-dimensional organoid culture techniques have emerged as a promising alternative. These organoid models can replicate the tumor microenvironments and retain the diversity of breast cancer, making them helpful in studying different stages of the disease. Optimized breast cancer organoid models under precise culture conditions offer numerous advantages, including faithful replication of in vivo traits of breast cancer cells, which have great potential in precision medicine, organ transplantation, disease modeling, gene therapy, and drug innovation.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Oncology
Liyang Zhang, Fangkun Liu, Nathaniel Weygant, Junxia Zhang, Ping Hu, Zailong Qin, Jingxuan Yang, Quan Cheng, Fan Fan, Yu Zeng, Yongjian Tang, Yusheng Li, Anliu Tang, Fengqiong He, Jun Peng, Weihua Liao, Zhongliang Hu, Min Li, Zhixiong Liu
Summary: Researchers developed an integrated system using patient-derived glioma cerebral organoids and xenograft models to understand glioma biology and predict response to chemotherapy drugs, providing a new strategy for personalized treatment of this deadly disease.
Review
Oncology
Johannes Betge, Rene Jackstadt
Summary: Patient derived organoids are valuable tools for modeling human diseases and understanding tumor biology. This review focuses on the recent developments in organoid-based research, using colorectal cancer as a prime example. The role of organoids in decoding cancer cell dynamics, tumor microenvironmental complexity, and the challenges faced in precision medicine and co-clinical trials are discussed.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Chemistry, Multidisciplinary
Zhichao Li, Haibo Xu, Yanqing Gong, Wei Chen, Yonghao Zhan, Lei Yu, Yangyang Sun, Aolin Li, Shiming He, Bao Guan, Yucai Wu, Gengyan Xiong, Dong Fang, Yuhui He, Qi Tang, Lin Yao, Zheng Hu, Hongbing Mei, Zhisong He, Zhiming Cai, Yinglu Guo, Xuesong Li, Liqun Zhou, Weiren Huang
Summary: The study successfully generated a library of 25 patient-derived UTUC organoid lines using a modified organoid culture system, retaining similar histological architectures and gene expression profiles. The research demonstrates that this model can be used to identify responses of UTUC organoids to different anticancer drugs and supports exploration of novel treatment strategies.
Article
Pharmacology & Pharmacy
Leonardo Delello Di Filippo, Juliana Hofstatter Azambuja, Jessyca Aparecida Paes Dutra, Marcela Tavares Luiz, Jonatas Lobato Duarte, Luiza Ribeiro Nicoleti, Sara Teresinha Olalla Saad, Marlus Chorilli
Summary: Glioblastoma multiforme (GBM) is the most common primary brain cancer with aggressive development and increasing resistance to drugs like temozolomide (TMZ). Functionalized nanocarriers can serve as an efficient and safe strategy for delivering TMZ, improving its biopharmaceutical properties and potentially repurposing its use in anti-GBM therapy.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Cell Biology
Min Soo Kim, Da-Hyun Kim, Hyun Kyoung Kang, Myung Geun Kook, Soon Won Choi, Kyung-Sun Kang
Summary: Novel brain organoid model systems have been developed from adult dermal fibroblast-derived neural stem cells, exhibiting characteristics of the human cerebral cortex for studying neuronal proliferation and maturation. When subjected to hypoxic injury, reoxygenation restored neuronal cell proliferation but not maturation in these neural organoids, suggesting new opportunities for drug screening and personalized modeling of neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Parisa Gazerani
Summary: Human organoids have revolutionized medical science by providing a way to understand diseases, test drugs, and develop novel treatments. Brain organoids in particular have the potential to investigate and treat various brain disorders, including migraines. By studying genetic factors and environmental stressors, as well as testing drug candidates, brain organoids can provide insights into migraine pathogenesis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Zhen Fang, Peijuan Li, Fengying Du, Liang Shang, Leping Li
Summary: Organoids are established through in vitro 3D culture and have the ability to mimic the structure and physiological functions of organs or tissues in vivo. They have gained significant attention and are considered reliable platforms for cancer research and treatment. Organoids have great potential in areas such as new drug development, drug screening, tumor mechanism research, and precision medicine. This review primarily focuses on recent advances in the application of organoids in cancer research and discusses the opportunities and challenges they face, aiming to provide insights for their future development.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Gianluca Costamagna, Giacomo Pietro Comi, Stefania Corti
Summary: Different research groups in academia have developed induced pluripotent stem cell-based protocols for generating neural organoids, providing new insights into brain biology and diseases. Challenges remain in adopting organoid technology for large-scale drug screening in the industry, but potential technical solutions include CRISPR, single-cell RNA sequencing, and machine learning for analyzing complex data sets. Rapid progress in the field will help translate organoid technology towards large-scale drug screening for neurological disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Hao Chen, Xin Jin, Tie Li, Zhuang Ye
Summary: Brain organoids, generated through the differentiation of pluripotent stem cells, provide a valuable platform for studying human brain development, disease modeling, and drug screening.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
George M. Ramzy, Maxim Norkin, Thibaud Koessler, Lionel Voirol, Mathieu Tihy, Dina Hany, Thomas McKee, Frederic Ris, Nicolas Buchs, Mylene Docquier, Christian Toso, Laura Rubbia-Brandt, Gaetan Bakalli, Stephane Guerrier, Joerg Huelsken, Patrycja Nowak-Sliwinska
Summary: This study proposes a new approach for designing personalized treatment for colorectal cancer patients by combining efficacy testing and mathematical modeling. The research identified four low-dose synergistic drug combinations that showed promising results in both primary and metastatic colorectal cancer models. This approach allows for the optimization of patient-tailored multi-drug combinations within a clinically relevant timeframe.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)