期刊
TALANTA
卷 223, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.121705
关键词
Aptamer; Circular DNA template; Ligate probe; Ochratoxin A; Electrochemical sensing; Amplification
资金
- Mashhad University of Medical Sciences
An aptasensor based on RCA was developed for electrochemical determination of OTA, showing superior analytical performance and sensitivity. Additionally, the technique has the potential to be applied for developing sensors to detect various toxins.
Herein, an aptasensor is presented for electrochemical determination of ochratoxin A (OTA) based on nontargettriggered production of rolling circular amplification (RCA). The surface of gold electrode is modified with thiolated complementary strand of aptamer (CS) as both capture probe and primer and OTA aptamer (Apt) as both sensing molecule and padlock probe (PLP). Following the addition of OTA, Apt/OTA conjugate is formed and detached from the electrode surface. Therefore, no RCA is produced after incubation of the modified electrode with T4 DNA ligase and phi29 DNA polymerase and a sharp current signal occurs. The analytical response ranged from 30 pM to 120 nM with detection limit of 5 pM. The designed aptasensor showed superior analytical performance in comparison with other approaches for OTA detection. Also, the approach exhibited good performance for OTA determination in spiked grape juice samples. The technique presented in this study, can be applied to develop sensors for detecting different toxins by replacing the relevant aptamers and complementary strands.
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