期刊
TALANTA
卷 224, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.121831
关键词
Enzymatic assay; Non-fluorescent substrate; Butyrylcholinesterase; Fluorescence change
资金
- National Natural Science Foundation of China [21173102, 21473072]
A novel strategy for biosensing BChE activity is developed using MnO2 nanosheets to modulate TH luminescence, enabling sensitive detection with a low detection limit. The feasibility of the biosensor in real samples analysis is also studied with satisfactory results.
In this paper, a novel strategy for biosensing butyrylcholinesterase (BChE) activity is developed based on manganese dioxide (MnO2) nanosheets to modulate the photoluminescence of thiamine (TH). The oxidase-like activity of MnO2 nanosheets enables them to catalyze the oxidation of non-fluorescent substrate TH to generate strong fluorescent thiochrome (TC). When the target BChE is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide (BTCh), MnO2 nanosheets are reduced by thiocholine to Mn2+, resulting in the loss of their oxidase-like activity and the reduction of TC fluorescence. Based on this, a BChE activity fluorescence biosensor is constructed utilizing the luminescence behavior variation of TH and the oxidase-like activity of MnO2 nanosheets. The fluorescence biosensor shows a sensitive response to BChE, and the detection limit reaches 0.036 U L-1. In addition, the feasibility of the biosensor in real samples analysis is studied with satisfactory results.
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