期刊
PLANT CELL AND ENVIRONMENT
卷 44, 期 6, 页码 1830-1845出版社
WILEY
DOI: 10.1111/pce.14040
关键词
lysine acetylome; perennial; Populus
资金
- Fundamental Research Funds for the Central Universities [2662019PY007]
- National Natural Science Foundation of China [31971676, 31800561]
- Opening Project of State Key Laboratory of Tree Genetics and Breeding [K2020103]
This study investigates the impact of lysine acetylation on hybrid poplar dormant buds, revealing that Kac modification affects the activity of key enzymes in primary metabolic pathways. The results suggest that Kac of enzymes could be an important strategy for reconfiguration of metabolic processes during bud dormancy release.
For perennials in boreal and temperate ecosystems, bud dormancy is crucial for survival in harsh winter. Dormancy is released by prolonged exposure to low temperatures and is followed by reactive growth in the spring. Lysine acetylation (Kac) is one of the major post-translational modifications (PTMs) that are involved in plant response to environmental signals. However, little information is available on the effects of Kac modification on bud dormancy release. Here, we report the dynamics of lysine acetylome in hybrid poplar (Populus tremula x Populus alba) dormant buds. A total of 7,594 acetyl-sites from 3,281 acetyl-proteins were identified, representing a large dataset of lysine acetylome in plants. Of them, 229 proteins were differentially acetylated during bud dormancy release and were mainly involved in the primary metabolic pathways. Site-directed mutagenesis enzymatic assays showed that Kac strongly modified the activities of two key enzymes of primary metabolism, pyruvate dehydrogenase (PDH) and isocitrate dehydrogenase (IDH). We thus propose that Kac of enzymes could be an important strategy for reconfiguration of metabolic processes during bud dormancy release. In all, our results reveal the importance of Kac in bud dormancy release and provide a new perspective to understand the molecular mechanisms of seasonal growth of trees.
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