Article
Horticulture
Jianmin Tang, Rong Zou, Xiao Wei, Dianpeng Li
Summary: In this study, the complete chloroplast genomes of five species of Ormosia were sequenced and compared. Ten hypervariable regions were identified, with petN-psbM showing the highest variation. Phylogenetic analysis revealed the evolutionary relationships within the genus Ormosia and provided valuable insights for future studies.
Article
Forestry
Jianmin Tang, Rong Zou, Taiguo Chen, Lipo Pan, Shujing Zhu, Tao Ding, Shengfeng Chai, Xiao Wei
Summary: The study analyzed the chloroplast genomes of six Cycas plants and found that the Cycas chloroplast genome is highly conserved with some significant variations at non-coding sites. By using phylogenetic analysis based on protein-coding genes, Cycas can be divided into four main groups. The findings of this study are valuable for evaluating genetic diversity, phylogenetic connections, and the advancement of wild germplasm resources.
Article
Plant Sciences
Xingxing Mao, Ji Wang, Nawal Shrestha, Yazhen Ma, Jianquan Liu
Summary: This study aimed to delimit species boundaries within the Rhododendron species complex, resulting in the identification of two distinct species and one putative hybrid group. The findings were based on morphological analyses and population genetic data, indicating a potential introgression event that formed the hybrid group. Further investigations are needed to validate the existence and characteristics of this hybrid group.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Ina Balke, Vilija Zeltina, Nikita Zrelovs, Ieva Kalnciema, Gunta Resevica, Rebeka Ludviga, Juris Jansons, Inga Morocko-Bicevska, Dalija Seglina, Andris Zeltins
Summary: This study identified, isolated, and sequenced a virus from a wild plantation of sea buckthorn for the first time, showing high similarity with viruses belonging to the genus Marafivirus. The viral genome is 6989 nucleotides in length and encodes two open reading frames, characterized by the polyprotein encoding ORF1 and the coat protein encoding ORF2. The expression of the major coat protein was found to be essential for virion assembly.
Article
Plant Sciences
Hoi-Yan Wu, Kwan-Ho Wong, Bobby Lim-Ho Kong, Tin-Yan Siu, Grace Wing-Chiu But, Stacey Shun-Kei Tsang, David Tai-Wai Lau, Pang-Chui Shaw
Summary: The pantropical genus Dalbergia consists of 269 species of trees, shrubs, and woody lianas. Due to illegal logging and trafficking driven by the high economic value of its heartwood, this genus is listed in CITES Appendices. However, species identification of Dalbergia timber and herbs is challenging but essential for CITES implementation. In this study, the chloroplast genomes of five Dalbergia species native to Hong Kong, including four medicinal plants, were sequenced and analyzed. Four divergence hotspots in the chloroplast genomes were identified as potential genetic markers for the identification of medicinal Dalbergia species.
Article
Food Science & Technology
Xingang Lyu, Yixuan Wang, Shiwei Gao, Xiao Wang, Wei Cao, Carlos L. Cespedes-Acuna
Summary: Sea buckthorn oil with the addition of sea buckthorn leaf extract showed improved stability and antioxidant properties. The best results were obtained when 2% SBLE was added to the SBO microcapsules prepared by spray drying. SBLE not only increased the antioxidant activity of SBO, but also showed better effectiveness compared to synthetic antioxidants.
Article
Agricultural Engineering
Xinran Chong, Yunlong Li, Minlei Yan, Yue Wang, Mingzhi Li, Yanwei Zhou, Hong Chen, Xiaoqing Lu, Fan Zhang
Summary: The genus Ilex includes economically important ornamental and medicinal plant species. This study describes the complete chloroplast genome sequences of three Ilex species and develops DNA barcodes for species identification. The findings provide resources for further research on the identification, genetic engineering, evolution, and phylogeny of Ilex species.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Forestry
Weihan Peng, Beibei Wang, Zhuolong Shen, Qirong Guo
Summary: The chloroplast genome of Pleioblastus ovatoauritus was analyzed and compared with other Pleioblastus species. Differences in genome structures, codon usage patterns, and SSR sites were found between Chinese and Japanese species. Nine SSR primers were developed for future identification and germplasm investigation of Pleioblastus genus.
Article
Biochemistry & Molecular Biology
Jiun-Nong Lin, Chung-Hsu Lai, Chih-Hui Yang, Yi-Han Huang, Alicja Wegrzyn
Summary: Bacteria in the genus Elizabethkingia have been identified as a cause of life-threatening infections in humans. In this study, the accuracy of 16S rRNA and rpoB gene sequences in identifying Elizabethkingia species was evaluated. The analysis showed that the complete rpoB gene sequencing is a useful marker for accurate identification of Elizabethkingia species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Qi Jin, Zitian Yang, Wenjing Yang, Xiaoyang Gao, Changning Liu
Summary: In this study, the LBD genes in Euphorbiaceae were analyzed, and the differences in gene number and phylogenetic relationship were discovered among different species. It was also found that LBD genes within the same clade have similar protein features and gene structures. Furthermore, these LBD genes may play important regulatory roles in flower development in Euphorbiaceae plants.
Article
Plant Sciences
Liwei Wu, Yingxian Cui, Qing Wang, Zhichao Xu, Yu Wang, Yulin Lin, Jingyuan Song, Hui Yao
Summary: The study showed that the chloroplast genomes of the five Crataegus species had a conserved structure and gene content. Complete chloroplast genome sequences were found to be more suitable for accurate identification and differentiation of Crataegus species. This suggests that the complete chloroplast genome can serve as a super-barcoding tool for authenticating Crataegus species.
Article
Biotechnology & Applied Microbiology
Niu Yanfei, Su Tai, Wu Chunhua, Deng Jia, Yang Fazhong
Summary: This study sequenced, assembled, and annotated the chloroplast genome of A. transsectum. The complete cp genome contains 131 genes and is similar in size and gene composition to the cp genomes of seven other Ranunculaceae species. Phylogenetic analysis showed that A. transsectum is closely related to certain Aconitum species.
Article
Horticulture
Zhenshan Liu, Jing Zhang, Yan Wang, Hao Wang, Lei Wang, Lu Zhang, Muran Xiong, Wen He, Shaofeng Yang, Qing Chen, Tao Chen, Ya Luo, Yong Zhang, Haoru Tang, Xiaorong Wang
Summary: In this study, a comparative genomics strategy was used to construct a database containing thousands of polymorphic SSR loci for genetic identification and trait-association analysis of Chinese cherry. The results showed that these markers exhibited high levels of informativeness and transferability, and were significantly associated with traits such as soluble solids and fruit size in Chinese cherry.
Article
Agronomy
Seyyed Javad Mousavizadeh, Juan Gil, Patricia Castro, Mohammad Reza Hassandokht, Roberto Moreno
Summary: This study assessed the genetic variability between wild related asparagus species and cultivated asparagus using molecular markers, revealing a certain genetic relationship between wild polyploid accessions and cultivated asparagus, while diploid accessions showed a greater genetic distance from them.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Yancui Jia, Youguo Li
Summary: This study identifies and analyzes the RALF gene family in Arabidopsis, soybean, Lotus, and Medicago. It reveals the evolutionary mechanism of RALFs in symbiosis and their potential involvement in nodule release.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)