Article
Microbiology
Georgi Merhi, Sara Amayri, Ibrahim Bitar, George F. Araj, Sima Tokajian
Summary: This study aimed to characterize multidrug resistant Enterobacter spp. and K. aerogenes isolates recovered from hospitalized patients in Lebanon. The isolates exhibited various resistance mechanisms and plasmid profiles. Multilocus sequence typing revealed the diversity within the isolates. Screening and continuous monitoring can effectively limit the dissemination of drug-resistant isolates in hospitalized patients.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Ricardo Oliveira, Joana Castro, Sonia Silva, Hugo Oliveira, Maria Jose Saavedra, Nuno Filipe Azevedo, Carina Almeida
Summary: This study identified a diverse pool of beta-lactam resistance genetic determinants in 102 multi-drug resistant K. pneumoniae isolates from patients in northern Portugal, leading to high levels of resistance to most beta-lactam antibiotics. The co-occurrence of multiple beta-lactam resistance genes in the majority of isolates serves as a serious warning of the problem of antimicrobial resistance.
Article
Engineering, Multidisciplinary
Cemile Bagkur, Emrah Guler, Bilgen Kaymakamzade, Evren Hincal, Kaya Suer
Summary: While antibiotic resistance is a growing concern, this study focuses on the future clinical situation of ESBL-producing K. pneumoniae. By using mathematical modeling, the study evaluates the severity of the issue and identifies critical points to prevent the spread of the ESBL-producing strain. The results show that the use of antibiotics and hospital admissions play a major role in the spread of ESBL-producing K. pneumoniae, and recovery from infections is the most effective way to limit its spread.
CMES-COMPUTER MODELING IN ENGINEERING & SCIENCES
(2022)
Article
Infectious Diseases
Asegdew Atlaw Woldeteklie, Habtamu Biazin Kebede, Abdurezak Ahmed Abdela, Yimtubezinash Woldeamanuel
Summary: This study evaluated the microbiological analysis and drug susceptibility testing of diabetic foot ulcer infections, highlighting the need for improved management.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biochemistry & Molecular Biology
Nuno M. M. Moura, Xavier Moreira, Eliana Sousa Da Silva, Joaquim Luis Faria, Maria G. P. M. S. Neves, Adelaide Almeida, Maria A. F. Faustino, Ana T. P. C. Gomes
Summary: Antimicrobial photodynamic therapy is a promising and innovative approach for the photoinactivation of microorganisms. This study demonstrates the excellent performance of porphyrin derivatives incorporated into materials for efficient photodynamic effect. These materials showed high efficacy in photoinactivating methicillin-resistant Staphylococcus aureus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Hasan Ejaz
Summary: This study investigated the role of outer membrane porin loss, AmpC, and carbapenemases in extended-spectrum beta-lactamase (ESBL)-producing and extensively drug-resistant (XDR) Klebsiella pneumoniae strains. The study identified the co-existence of ESBLs, AmpC beta-lactamases, and carbapenemases in a significant number of K. pneumoniae strains, with a high prevalence of OmpK35 and OmpK36 porin loss. The findings suggest that these resistance mechanisms contribute to the emergence of XDR and pan drug resistance in K. pneumoniae.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Infectious Diseases
Shazia Naheed, Irum Umar Din, Muhammad Usman Qamar, Nasir Rasool, Matloob Ahmad, Muhammad Bilal, Aqsa Khalid, Gulraiz Ahmad, Sami A. Al-Hussain, Magdi E. A. Zaki
Summary: This study developed butyl 2-bromoisonicotinate and tested its antibacterial effectiveness against ESBL-producing E. coli ST405 and MRSA. Synthetic compound synthesis can overcome the limitations of natural sources and increase the potential for discovering new medicinal compounds. The results showed that butyl 2-bromoisonicotinate displayed antibacterial efficacy against both resistant strains.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Shang Wang, Shan Wang, Ying Tang, Guoyu Peng, Tongyu Hao, Xincheng Wu, Jiehong Wei, Xinying Qiu, Dewang Zhou, Shimao Zhu, Yuqing Li, Song Wu
Summary: This study established a system that combines PCR with CRISPR-LbCas12a for the detection of Klebsiella pneumoniae and ESBL-positive strains' nucleic acid. The system showed excellent detection specificity and sensitivity in both bench work and clinical samples. The application of this method can meet different detection requirements where qPCR is not accessible.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Mariana Castanheira, John H. Kimbrough, Sean DeVries, Rodrigo E. Mendes, Helio S. Sader
Summary: The temporal and longitudinal trends of beta-lactamases and their associated susceptibility patterns were analyzed for Escherichia coli and Klebsiella pneumoniae isolates consecutively collected in 56 United States hospitals during 2016-2020. The results showed that extended-spectrum beta-lactamases (ESBLs) were detected among 88.0% of the isolates without carbapenem resistance, and bla(CTX-M-15) was the most common ESBL variant. Significant increases in ESBL rates were noted among bloodstream infection and K pneumoniae isolates due to the increase in bla(CTX-M). Carbapenem resistance and carbapenemase genes were also detected, but their prevalence remained low. Ceftazidime-avibactam and carbapenems were highly active against ESBL producers without carbapenem resistance. Ceftazidime-avibactam also showed good inhibition against carbapenem-resistant isolates. Overall, the study highlights the importance of continuous surveillance of beta-lactamase-producing isolates.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Hiroyuki Yamamoto, Katsuya Hashimoto, Hiroyuki Yamada, Yoshihiko Ikeda, Takashi Takahashi, Toru Hashimoto
Summary: This case study demonstrates the importance of using 4D TEE in diagnosing MAC-related IE and annular abscess, as well as the limitations of the modified Duke criteria in cases of definite IE with dual bacteremia.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Oncology
Yaxiong He, Jiaying Pang, Zengjun Yang, Mengxue Zheng, Yanlan Yu, Zhiyong Liu, Baohua Zhao, Guangyun Hu, Rui Yin
Summary: This study evaluates the suppression efficacy of toluidine blue O-mediated photodynamic antimicrobial chemotherapy (PACT) on mature Methicillin-resistant Staphylococcus aureus (MRSA) biofilm in vitro. The results demonstrate that PACT can destroy the biofilm structure, reduce its virulence, and depress its self-recovery.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Environmental Sciences
Xiaojie Xu, Min Yang, Yunhan Jiang, Ningyao Tao, Yulong Fu, Jiahui Fan, Xin Xu, Huixiang Shi, Zhan Lu, Chaofeng Shen
Summary: The spread of antibiotic resistant pathogens and antibiotic resistance genes in the environment is a serious threat to public health. Existing methods are ineffective in removing them, but a new acridine-based photosensitizer called YM-3 has been developed in this study, which can effectively inactivate antibiotic resistant pathogens and reduce antibiotic resistance genes by generating singlet oxygen. YM-3 has shown a high inactivation rate for methicillin-resistant Staphylococcus aureus and carbapenem-resistant Acinetobacter baumannii, and it can effectively reduce the copy numbers of antibiotic resistance genes in these bacteria. It also has a long-lasting bactericidal effect, making it suitable for disinfection in various environments.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Aly E. Abo-Amer, Sanaa M. F. Gad El-Rab, Eman M. Halawani, Ameen M. Niaz, Mohammed S. Bamaga
Summary: This research investigated the prevalence of MRSA in Taif, Saudi Arabia and examined the relationship between antimicrobial sensitivity patterns and resistance genes. The study also found that silver nanoparticles showed high antimicrobial activity and synergistic effect with penicillin against MRSA isolates.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Zahid Hayat Mahmud, Salman Zahir Uddin, M. Moniruzzaman, Sobur Ali, Monir Hossain, Md Tamzid Islam, Dorin Teresa D. Costa, Mohammad Rafiqul Islam, Md Shafiqul Islam, Md Zakiul Hassan, Li-Ann Ong, Catrin E. Moore, Katrina J. Charles, Dinesh Mondal, Bruno Silvester Lopes, Shahana Parveen
Summary: The emergence of virulent extended spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KP) including carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital-acquired infections has led to significant morbidity and mortality worldwide. A study conducted in tertiary care hospitals in Bangladesh revealed that these bacterial strains exhibited high levels of antibiotic resistance, could form biofilms, and contained various antibiotic resistance and virulence genes. The findings are important for improving patient care, infection control, and the development of public health policies.
Article
Food Science & Technology
Alba Martinez-Laorden, Celia Arraiz-Fernandez, Elena Gonzalez-Fandos
Summary: The aim of this study was to assess the microbial safety and quality of fresh turkey meat sold in Spain, focusing on antibiotic-resistant bacteria such as methicillin-resistant S. aureus, ESBL-producing E. coli, and K. pneumoniae. The results showed varying levels of contamination, with Enterobacteriaceae being the most prevalent. Antibiotic resistance was also detected among the isolates, including resistance to critical antibiotics. These findings highlight the importance of monitoring and controlling bacterial contamination in fresh turkey meat.
Article
Environmental Sciences
Robert Bajgar, Anna Moukova, Nela Chalupnikova, Hana Kolarova
Summary: The study focused on the impact of longer-wavelength UV components (UVA and UVB) on skin cells. Results showed that UVB radiation may cause more severe damage, indicating a need for more cautious application.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Immunology
Michal Krupka, Tereza Matusu, Helena Sutova, Katerina Wezdenkova, Renata Vecerova, Yvona Smesna, Milan Kolar, Hana Bilkova Frankova, Jana Krivankova, Miroslav Jorenek, Zdenek Novak, Milan Raska, Ondrej Holy
Summary: This study analyzed data from four laboratories in the Olomouc Region, Czech Republic, and found significant differences in measles positive results depending on the individual laboratories and detection methods used. However, individuals born before the introduction of vaccination showed higher levels of antibodies.
Article
Biology
Yuliya Morozova, Iva Voborna, Radovan Zizka, Katerina Bogdanova, Renata Vecerova, Dominik Rejman, Milan Kolar, Duy Dinh Do Pham, Pavel Holik, Roman Mostek, Matej Rosa, Lenka Pospisilova
Summary: The study focused on the ex vivo effects of second- and third-generation LPPOs on Enterococcus faecalis in root canal samples, comparing them with other disinfectants. Results showed that LPPOs demonstrated superior bactericidal effects compared to the tested disinfectants by reducing planktonic bacterial cells and inhibiting biofilm formation.
Article
Engineering, Biomedical
Martin Cernan, Tomas Szotkowski, Jaromir Hubacek, Milan Kolar, Edgar Faber, Karel Indrak, Tomas Papajik
Summary: This study retrospectively analyzed the incidence of infectious complications in AML patients without routine antibiotic prophylaxis in a clinical site in Czech Republic over a 15-year period. The results showed that infectious complications were common, but infectious mortality was comparable to patients with prophylaxis. Additionally, achieving complete remission had a significant effect on the duration of febrile episodes and overall survival, while infection or fever of unknown origin at diagnosis increased the colonization of multi-drug resistant bacterial strains and prolonged antibiotic therapy.
BIOMEDICAL PAPERS-OLOMOUC
(2023)
Article
Multidisciplinary Sciences
Lucie Hochvaldova, David Panacek, Lucie Valkova, Robert Prucek, Vera Kohlova, Renata Vecerova, Milan Kolar, Libor Kvitek, Ales Panacek
Summary: This study reports an effective way of enhancing and restoring the antibacterial activity of inactive antibiotics by using a cyanographene/Ag nanohybrid. The combined treatment showed synergistic and partial synergistic effects against multiresistant bacterial strains, indicating the potential of the nanohybrid in overcoming antibiotic resistance. The study also revealed that the interaction between the antibiotic's mode of action and the bacterial resistance mechanism strongly influenced the efficacy of the combined treatment.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Biology
Milan Kolar
Article
Biology
Petr Jakubec, Katerina Fiserova, Samuel Genzor, Milan Kolar
Summary: According to the study, COVID-19 patients hospitalized 29 days or more after infection most commonly had respiratory tract infections, with pneumonia being the most frequent complication. Patients with infectious diseases were hospitalized sooner after testing positive for PCR and had more non-respiratory complications. The overall in-hospital mortality rate was 5.1%.
Article
Chemistry, Medicinal
Duy Dinh Do Pham, Viktor Mojr, Michaela Helusova, Gabriela Mikusova, Radek Pohl, Eva Davidova, Hana Sanderova, Dragana Vitovska, Katerina Bogdanova, Renata Vecerova, Miroslava Htoutou Sedlakova, Radovan Fiser, Petra Sudzinova, Jiri Pospisil, Oldrich Benada, Tomas Krizek, Adela Galandakova, Milan Kolar, Libor Krasny, Dominik Rejman
Summary: The novel antibacterial compounds LEGO-LPPOs were developed based on LPPOs, showing activity against various bacteria with low cytotoxicity and resistance development potential. This new class of compounds demonstrates efficacy against multiresistant strains and persisters, by depleting membrane potential and creating pores in the cytoplasmic membrane.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Infectious Diseases
Lenka Doubravska, Miroslava Htoutou Sedlakova, Katerina Fiserova, Vendula Pudova, Karel Urbanek, Jana Petrzelova, Magdalena Roderova, Katerina Langova, Kristyna Mezerova, Pavla Kucova, Karel Axmann, Milan Kolar
Summary: This observational retrospective study analyzed the changes in bacterial pathogens and their resistance to antibiotics among intensive care patients in a Czech hospital during the COVID-19 pandemic. The study found that there were shifts in the distribution and resistance of bacterial pathogens, possibly due to antibiotic selection pressure and clonal spread of bacteria.
Article
Biology
Martin Cernan, Tomas Szotkowski, Jiri Minarik, Milan Kolar, Pavel Sauer, Vojtech Latal, Jana Zapletalova, Tomas Papajik
Summary: This paper analyzes the impact of COVID-19 vaccination on the course of breakthrough infection in patients with hematologic malignancies. The results show that vaccinated patients have a milder clinical course, lower hospitalization rates, and lower mortality.
Article
Biochemistry & Molecular Biology
Anna Moukova, Lukas Malina, Hana Kolarova, Robert Bajgar
Summary: The study focused on the protective effects of eight organic UV-absorbing compounds on skin cells against UVA and UVB radiation. Only a few compounds, such as trans-urocanic acid and hyperoside, had a significant impact on UV-induced cell damage. Hyperoside was found to be a highly effective UV-protective compound, especially against UVA radiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Josef Chudacek, Petr Spicka, Milan Kolar, Martin Stasek, Stefan Kolcun, Dusan Klos, Kristyna Hricova, Patrik Mlynarcik, Vendula Pudova, Olga Klementova, Rostislav Horacek
Summary: This study extends the existing knowledge of hospital-acquired pneumonia (HAP) complicating secondary peritonitis by providing detailed microbiological characteristics, including the identification of isolated bacterial pathogens and their potential sources. The study emphasizes the importance of initial antibiotic therapy effective against enterobacteria, ESBL and AmpC β-lactamases producing strains, Pseudomonas aeruginosa, and Enterococcus faecalis in patients with secondary peritonitis complicated by HAP. Monitoring the respiratory tract bacterial microflora in these patients is also highlighted as important.
Review
Pharmacology & Pharmacy
Viktor Sebek, Pavel Anzenbacher, Dominik Rejman, Alena Spicakova, Milan Kolar
Summary: Lipophosphonoxins (LPPOs) are a new group of antibiotics that target bacterial cell membranes. They have bactericidal activity against both Gram-positive and Gram-negative bacteria, with better selectivity and less resistance compared to previous generations. LPPOs have shown potential therapeutic effects in bone cement infections and wound infections.
Article
Biochemistry & Molecular Biology
Ludmila Zarska, Zuzana Mala, Katerina Langova, Lukas Malina, Svatopluk Binder, Robert Bajgar, Petr Henke, Jiri Mosinger, Hana Kolarova
Summary: The study showed that using TPP photosensitizers embedded in polystyrene nanoparticles for PDT can generate reactive oxygen species, leading to cytotoxicity only after activation by blue light (414nm), with a significantly higher effect on tumor cells compared to non-tumor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Lucie Hochvaldova, Renata Vecerova, Milan Kolar, Robert Prucek, Libor Kvitek, Lubomir Lapcik, Ales Panacek
Summary: This review thoroughly analyzes and evaluates the combined antibacterial effect of nanomaterials and antibiotics. The study reveals that the antibiotic's mode of action and bacterial resistance mechanism determine the effectiveness of the combined treatment. Only specific combinations of nanomaterials and antibiotics can enhance and restore the antibacterial effectiveness against resistant bacteria.
NANOTECHNOLOGY REVIEWS
(2022)