Article
Biochemistry & Molecular Biology
Cheng Zhen, Xinguo Hua, Xue Jiang, Guimin Tong, Chunming Li, Chuanping Yang, Yuxiang Cheng
Summary: Fasciclin-like arabinogalactan proteins (FLAs) play an important role in plant development and adaptation to the environment. In this study, 50 PtrFLA genes were identified in poplar, and they were classified into four groups: A to D. Most PtrFLAs in group A were highly expressed in developing xylem. The loss of PtrFLA40 and PtrFLA45 increased stem length and diameter in ptrfla40ptrfla45 double mutants, and also led to enlargement of xylem fibers and vessels. Additionally, the mutants showed an increase in wood lignin content and up-regulation of lignin biosynthesis-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
W. Patrick Bewg, Scott A. Harding, Nancy L. Engle, Brajesh N. Vaidya, Ran Zhou, Jacob Reeves, Thomas W. Horn, Nirmal Joshee, Jerry W. Jenkins, Shengqiang Shu, Kerrie W. Barry, Yuko Yoshinaga, Jane Grimwood, Robert J. Schmitz, Jeremy Schmutz, Timothy J. Tschaplinski, Chung-Jui Tsai
Summary: In this study, a single gRNA was used to target conserved sequences for efficient mutagenesis of a multigene family and the recovery of specific mutants in poplar plants. Deep sequencing and PCR analyses confirmed successful editing across multiple targets with minimal off-target mutations. Additionally, the study revealed a potential new role for non-glandular trichomes in poplar plants.
Article
Materials Science, Paper & Wood
Bingwei Chen, Shengcheng Zhai, Yu'na Kan, Xiaodong Fan, Xinzhou Wang, Biao Pan, Changtong Mei, Junji Sugiyama
Summary: Phloem fibers from the bark of Pteroceltis tatarinowii are the main material for Xuan zhi, Chinese traditional handmade paper. Research on these fibers helps uncover scientific meanings and preserve cultural heritage. Age affects the anatomy, morphology, cellulose, and mechanical characteristics of the fibers, with 5-year-old fibers showing higher crystallinity and indentation modulus.
Article
Biochemistry & Molecular Biology
Jiuling Zhu, Huimin Xu, Hui Song, Xiang Li, Ning Wang, Junli Zhao, Xiaojing Zheng, Kwang-Youn Kim, Hui Zhang, Qinwen Mao, Haibin Xia
Summary: In this study, stable grna knockout zebrafish models were generated using CRISPR/Cas9-mediated genome editing, exhibiting neurodevelopmental defects and behavior changes. The phenotypes of grna knockout zebrafish could be rescued with the Tol2 system carrying the grna gene, demonstrating the potential utility of this model for studying PGRN function and high-throughput drug screening for disease therapy.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Joseph Carver, Marie Kern, Peggy Ko, Midori Greenwood-Goodwin, X. Christopher Yu, Dana Duan, Danming Tang, Shahram Misaghi, Simon Auslaender, Ben Haley, Inn H. Yuk, Amy Shen
Summary: Engineering CHO cells using CRISPR/Cas9 knockout technology requires the delivery of gRNA and Cas9 enzyme for efficient gene targeting. Sequential transfections can effectively target and knockout up to 10 genes, significantly reducing the time and effort required.
BIOTECHNOLOGY PROGRESS
(2022)
Article
Biochemical Research Methods
Eun-Kyung Bae, Hyunmo Choi, Ji Won Choi, Hyoshin Lee, Sang-Gyu Kim, Jae-Heung Ko, Young-Im Choi
Summary: CRISPR/Cas9 was successfully used to induce target mutations in hybrid poplars, showing a mutation efficiency of around 74.5%. This technique has the potential to improve tree quality by enhancing biomass or stress tolerance.
TRANSGENIC RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Lin Yuan, Shichen Bu, Meng Du, Yilong Wang, Chenhui Ju, Dandan Huang, Wenjing Xu, Xin Tan, Minglu Liang, Shan Deng, Liu Yang, Kai Huang
Summary: This study investigated the role of RNF207 in the development of pathological cardiac hypertrophy and dysfunction. The results showed that RNF207 exacerbated cardiac hypertrophy and fibrosis, while RNF207 knockdown alleviated these conditions.
CARDIOVASCULAR RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Wenjing Xu, Sheli Zhang, Ruofan Shen, Zhikun Peng, Baozhong Liu, Jun Li, Zhanying Zhang, Baojun Li
Summary: This study investigates the interfacial structure between metal nanoparticles and metal oxides and demonstrates the importance of high-density interfaces in maximizing catalytic activity. By synthesizing a Cu-Co3O4 nanoparticles interfacial structure through pyrolysis and moderate oxidation, the researchers discover the synergistic effect between Cu and Co3O4 nanoparticles at their interface. Density functional theory calculations and in situ Raman spectroscopy reveal the catalytic mechanism of dual active sites.
ENERGY & ENVIRONMENTAL MATERIALS
(2023)
Article
Computer Science, Information Systems
Xiaofeng Jia, Wenjing Xu, Pan Deng, Song Gao, Lin Tang, Yuhang Wang, Min Zhang, Lulu Bao, Dingding Lin
Summary: This paper proposes a cross-organisational data-sharing framework based on Blockchain-Probes using distributed digital identity technology, which achieves secure and credible data sharing. In practical application, the framework improves efficiency and accuracy while reducing total cost.
Article
Chemistry, Physical
Ming Qu, Zhe Chen, Zhiyi Sun, Danni Zhou, Wenjing Xu, Hao Tang, Hongfei Gu, Tuo Liang, Pengfei Hu, Guangwen Li, Yu Wang, Zhuo Chen, Tao Wang, Binbin Jia
Summary: The atomic-level interfacial regulation of single metal sites through heteroatom doping can significantly improve the characteristics of the catalyst and obtain surprising activity. In this study, nickel single-site catalysts with dual-coordinated phosphorus and nitrogen atoms were developed and showed excellent catalytic performance in CO2 reduction reaction.
Article
Computer Science, Artificial Intelligence
Xu Wenjing, Zilu Cai
Summary: Art design is a means of expressing human sentiments and emotions, and the introduction of AI and machine learning has revolutionized the creation, consumption, and distribution of art. These technologies have also proven to be successful in developing students' creative talents in art-related courses. By utilizing information technology, designers are able to create and design artwork more efficiently. Artificial intelligence and machine learning play a positive role in the evolution and creation of interactive and intelligent digital art, accurately conveying human emotions and sentiments. The study provides a comprehensive overview of AI-based applications in art design, demonstrating their effectiveness.
Article
Computer Science, Information Systems
Qian Cao, Xiulan Hao, Huajian Ren, Wenjing Xu, Shiluo Xu, Charles Jnr Asiedu
Summary: This paper proposes a textual Emotion-Cause Pair causal relationship detection method fused with graph attention network. The model utilizes a structural relationship graph to propagate causal features and capture dependency information between sentences. Experimental results show that the proposed model outperforms existing methods.
WORLD WIDE WEB-INTERNET AND WEB INFORMATION SYSTEMS
(2023)
Article
Polymer Science
Lijuan Long, Wenjing Xu, Tao Xu, Guomin Xu, Yushu Xiang, Chunyan Shan, Min He, Shuhao Qin, Jie Yu
Summary: This study systematically and comparatively investigated the flame retardancy of PLA/PBAT composites containing DOPO derivatives. Results showed that different structures of the derivatives exhibit different effects, with DIDOPO demonstrating better flame inhibition and DOPO-HQ providing superior barrier and protective functions.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Physical
Wenjing Xu, Yidong Sun, Jiaqi Zhou, Maoqi Cao, Jun Luo, Haili Mao, Pengfei Hu, Hongfei Gu, Huazhang Zhai, Huishan Shang, Zhi Cai
Summary: This study develops a cost-effective and high-efficiency catalyst for oxygen reduction reaction (ORR) by constructing atomically dispersed Co atoms on porous N-enriched graphene material. The catalyst exhibited superior ORR performance and impressive long-term durability, suggesting its potential applications in fuel cells and metal-air batteries.
Article
Chemistry, Physical
Wenjing Xu, Jiwu Li, Qiaoyun Liu, Wei Li, Wenfang Zhang, Baojun Li
Summary: Developing high-performance and low-cost catalysts for hydrogen generation from hydrogen-storage materials is a major challenge. This study created a ternary interface of copper-iron-nickel by embedding copper nanoparticles in an iron-nickel metal-organic framework (MOF) through a solvothermal process. The optimized Cu@FeNi-MOF catalyst showed outstanding stability and excellent catalytic activity, with a turnover frequency surpassing all reported catalysts containing Cu, Fe or Ni. The unprecedented activity was attributed to the presence of three-atom dual-active sites at the Cu-Fe delta+-Ni2+ interfaces.
APPLIED SURFACE SCIENCE
(2023)
Article
Plant Sciences
Chaofan Chen, Yi Zhang, Jianfa Cai, Yuting Qiu, Lihong Li, Chengxu Gao, Yiqun Gao, Meiyu Ke, Shengwei Wu, Chuan Wei, Jiaomei Chen, Tongda Xu, Jiri Friml, Junqi Wang, Ruixi Li, Daiyin Chao, Baocai Zhang, Xu Chen, Zhen Gao
Summary: Multi-copper oxidases SKU5 and SKS1 modulate ROS homeostasis in root apoplast to regulate cell wall formation. Loss of SKU5 and SKS1 function leads to aberrant cell wall structure and excessive ROS production, which can be rescued by decreasing ROS level or inhibiting NADPH oxidase activity.
Article
Materials Science, Multidisciplinary
Xiangyu Xu, Ziqian Sheng, Jueli Shi, Xin Chen, Yaxin He, Wenjing Xu, Xu Zhang, Duanyang Chen, Hongji Qi, Kelvin H. L. Zhang
Summary: The bandgap of (AlxGa1-x)(2)O-3 thin films can be modulated by varying the Al contents, resulting in high-performance photodetectors for solar-blind UV bands. The detection cut-off wavelengths and response peaks of the photodetectors can be tuned by changing the Al contents. The wide variations of the bandgap are mainly due to the upshift of the conduction band edges induced by Al 3s state hybridization.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Plant Sciences
Kaori Okada, Wenjing Xu, Kohei Mishina, Youko Oono, Tsuneo Kato, Kiyoshi Namai, Takao Komatsuda
Summary: This study revealed the mechanism of resistance against the Japanese soil-borne wheat mosaic virus. Using quantitative PCR assay, it was found that the resistance acts directly against the virus rather than its fungal vector. By comparing different barley cultivars and wild barley, different genes were identified for the suppression of the virus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Wenjing Xu, Meng Yang, Xuanlong Du, Hao Peng, Yue Yang, Jitao Wang, Yewei Zhang
Summary: We developed a novel multifunctional nanoplatform with superior therapeutical efficiency for hepatocellular carcinoma (HCC) by combining molecular targeted therapy and phototherapy. The nanoplatform not only has excellent biocompatibility but also can be used for photothermal therapy and photodynamic therapy. In vivo experiments showed that the nanoplatform exhibited multimode imaging capabilities, which can be used for tumorous diagnosis and intraoperative navigation.
Article
Neurosciences
Guo-Kun Zhou, Wen-Jing Xu, Yi Lu, Yan Zhou, Chen-Zhang Feng, Jiang-Tao Zhang, Shi-Yu Sun, Ruo-Meng Wang, Tong Liu, Bin Wu
Summary: This study identifies agmatine as a novel mediator of itch and demonstrates that it induces itch through the activation of ASIC3. Targeting neuronal ASIC3 signaling may represent a novel strategy for the treatment of itch.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Plant Sciences
Rui Xu, Zhuolin Liu, Xiaohong Wang, Yihua Zhou, Baocai Zhang
Summary: Xylan clustering on the pollen surface is essential for the formation of sculpted exine patterns in flowering plants. Xylan synthase proteins mediate the directional assembly of xylan, which governs species-specific exine patterning.
