期刊
ENGINEERING IN LIFE SCIENCES
卷 17, 期 3, 页码 333-344出版社
WILEY
DOI: 10.1002/elsc.201500153
关键词
Biodiesel; Biorefineries; Fermentation; Microbial oil production; Yeast isolation
资金
- National Council for Scientific and Technological Development of the Ministry of Science, Technology and Innovation (CNPq/MCTI) through the Special Visiting Researcher fellowship [313772/2013-4]
Microbial oil production has received significant attention as a potential precursor for the production of biofuels, oleochemicals and food products. In this study, six oleaginous yeasts, isolated from fruits, were selected based on their ability to accumulate high intracellular content of microbial oil (20-48% w/w of total dry weight). The highest content of saturated fatty acids was 68.7% (w/w), whereas the highest content of oleic acid was 62.7% (w/w). Furthermore, nutrient-rich hydrolysates produced via enzymatic hydrolysis of flour-richwaste streams generated by a confectionery industry were evaluated as fermentation media for microbial oil production via fed-batch bioreactor cultures using one of the most promising isolates, namely VV-D4. A total dry weight of 40 g/L with a microbial oil content of 39% (w/w) was produced by isolate VV-D4. Critical biodiesel properties were estimated based on the fatty acid composition and correlated with the international standards. The microbial oil produced by the new isolates could be potentially used for biodiesel production.
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