期刊
NANO LETTERS
卷 21, 期 5, 页码 2132-2140出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c04883
关键词
plasmon-enhanced fluorescence; surface-enhanced fluorescence; metal-enhanced fluorescence; biosensors; fluorescence nanoprobes; microarray-based microRNA detection
类别
资金
- BioNano Health-Guard Research Center - Ministry of Science and ICT (MSIT) of Korea as Global Frontier Project [H-GUARD_2013-M3A6B2078947]
- National Research Foundation of Korea (NRF) - Korean government (MIST) [NRF-2017R1A5A1015365]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT, and Future Planning [2017R1A2B3008478]
- National Research Foundation of Korea [4120200213576, 4199990214002, 2017R1A2B3008478] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study focuses on designing and synthesizing fluorescence-amplified nanocuboids (FANCs) with highly enhanced and controlled plasmon-enhanced fluorescence (PEF) signals, as well as fluorescent silica shell-coated FANCs (FS-FANCs) for biosensing applications. By modifying DNA labeled Au nanorods and forming ultraflat Ag shells, the authors achieved a significant fluorescence enhancement factor and high sensitivity for miRNA detection.
There has been enormous interest in understanding and utilizing plasmon-enhanced fluorescence (PEF) with metal nanostructures, but maximizing the enhancement in a reproducible, quantitative manner while reliably controlling the distance between dyes and metal particle surface for practical applications is highly challenging. Here, we designed and synthesized fluorescence-amplified nanocuboids (FANCs) with highly enhanced and controlled PEF signals, and fluorescent silica shell-coated FANCs (FS-FANCs) were then formed to fixate the dye position and increase particle stability and fluorescence signal intensity for biosensing applications. By uniformly modifying fluorescently labeled DNA on Au nanorods and forming ultraflat Ag shells on them, we were able to reliably control the distance between fluorophores and Ag surface and obtained an similar to 186 fluorescence enhancement factor with these FANCs. Importantly, FS-FANCs were utilized as fluorescent nanoparticle tags for microarray-based miRNA detection, and we achieved >10(3)-fold higher sensitivity than commercially available chemical fluorophores with 100 aM to 1 pM dynamic range.
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