Article
Environmental Sciences
Xiaoqiang Liu, Jingru Zuo, Jiang Teng, Le Yang, Jingjing Guo, Lianjie Liu, Pei Li
Summary: This study investigated the prevalence and multidrug-resistant (MDR) phenotype of Staphylococcus aureus in raw milk samples and farm environments. The antimicrobial and antibiofilm activities of luteolin against MDR S. aureus were also explored. The results showed that luteolin can inhibit biofilm formation, reduce cell viability, and affect gene expression related to biofilm formation in S. aureus.
ENVIRONMENTAL POLLUTION
(2023)
Review
Biochemistry & Molecular Biology
Vincent Jean-Pierre, Agathe Boudet, Pauline Sorlin, Quentin Menetrey, Raphael Chiron, Jean-Philippe Lavigne, Helene Marchandin
Summary: Staphylococcus aureus is a major human pathogen that can survive and cause diseases in various clinical settings, including Cystic Fibrosis. In Cystic Fibrosis, S. aureus is the most commonly identified opportunistic pathogen, and its colonization/infection can become chronic in about one third of patients. S. aureus's ability to form biofilm is a crucial factor contributing to its persistence and protection in the lungs of CF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Taisir Saber, Mohamed Samir, Rasha M. El-Mekkawy, Eman Ariny, Sara Ramadan El-Sayed, Gamal Enan, Sawasn H. Abdelatif, Ahmed Askora, Abdallah M. A. Merwad, Yasmine H. Tartor
Summary: This study aimed to investigate the frequency, antimicrobial resistance, and biofilm-forming ability of MRSA and VRSA in RTE meat and food handlers. The presence of these bacteria in these sources highlights the potential danger and calls for stricter hygiene practices and antimicrobial surveillance plans to reduce the risk of spread.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Henan Guo, Yucui Tong, Junhao Cheng, Zaheer Abbas, Zhongxuan Li, Junyong Wang, Yichen Zhou, Dayong Si, Rijun Zhang
Summary: The overuse of antibiotics leading to drug-resistance and multidrug-resistance has become a major concern. Staphylococcus aureus, as a representative of drug-resistant pathogens, can cause a variety of infections and adapt to diverse environments through phenotypic changes like biofilm and SCVs. This article reviews the development of drug-resistance, explores classical mechanisms, and focuses on the non-classical resistance mechanisms triggered by biofilm and SCVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Kira L. Tomlinson, Tania Wong Fok Lung, Felix Dach, Medini K. Annavajhala, Stanislaw J. Gabryszewski, Ryan A. Groves, Marija Drikic, Nancy J. Francoeur, Shwetha H. Sridhar, Melissa L. Smith, Sara Khanal, Clemente J. Britto, Robert Sebra, Ian Lewis, Anne-Catrin Uhlemann, Barbara C. Kahl, Alice S. Prince, Sebastian A. Riquelme
Summary: The authors demonstrate that the pathogen Staphylococcus aureus induces a distinct airway immunometabolic response dominated by the release of itaconate. This metabolite potentiates extracellular polysaccharide synthesis and biofilm formation in S. aureus, potentially facilitating chronic infection.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Bhawandeep Kaur, Jeena Gupta, Sarika Sharma, Divakar Sharma, Sandeep Sharma
Summary: Staphylococcus aureus is a common pathogen causing various infections, with Methicillin-Resistant Staphylococcus aureus (MRSA) being particularly concerning. The primary antimicrobial resistance mechanisms in S. aureus involve efflux pumps and biofilm formation, with quorum sensing and drug efflux playing crucial roles. Efflux pump inhibitors (EPIs) have been identified as a promising approach to combat bacterial resistance, by inhibiting drug efflux mechanisms and transport of quorum sensing signalling molecules.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Abeni Beshiru, Isoken H. Igbinosa, Etinosa O. Igbinosa
Summary: The study revealed the prevalence of multi-drug resistant Staphylococcus aureus strains in ready-to-eat shrimp samples, with high levels of virulence determinants and antibiotic resistance genes. The findings highlight the significant public health risk posed by contaminated seafood.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Jingsha Dai, Jiahui Huang, Shi Wu, Feng Zhang, Yuanyu Li, Dongli Rong, Qinghua Ye, Miao Zhao, Qihui Gu, Youxiong Zhang, Xianhu Wei, Jumei Zhang, Qingping Wu
Summary: The study aimed to determine the prevalence and characterization of Staphylococcus aureus in shrimp samples from 39 cities in China. Results showed that 28% of the samples from 24 cities were positive for S. aureus, with most below 110 MPN/g. Antibiotic susceptibility testing revealed high rates of multidrug resistance. Genes associated with antibiotic resistance, biofilm production, and virulence factors were detected in the isolates. Molecular typing identified dominant genetic types, some of which have been linked to human infections globally.
Article
Pharmacology & Pharmacy
Xinyun Kang, Qiang Ma, Guilai Wang, Na Li, Yanni Mao, Xin Wang, Yuxia Wang, Guiqin Wang
Summary: This study identified the gene clfB to be closely related to biofilm formation in Staphylococcus aureus. The study revealed the mode of binding between Quercetin and ClfB, and demonstrated that Quercetin can inhibit the formation of S. aureus biofilm.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Infectious Diseases
Seham Abdel-Shafi, Heba El-Serwy, Yehia El-Zawahry, Maysaa Zaki, Basel Sitohy, Mahmoud Sitohy
Summary: In this study, 66 Staphylococcus bacterial isolates were examined for biofilm development and gene characteristics. The presence of ica genes was not the only factor in biofilm formation mechanisms. CoPS was the most common species, with S. aureus being the major resistant strain. These findings have important implications for the prevention and treatment of Staphylococcus infections.
Article
Microbiology
Fengjia Chen, Jing Zhang, Hyun Jung Ji, Min-Kyu Kim, Kyoung Whun Kim, Jong-Il Choi, Seung Hyun Han, Sangyong Lim, Ho Seong Seo, Ki Bum Ahn
Summary: Deinococcus radiodurans is a highly resistant bacterium with cellular constituents, such as DeinoPol, playing a key role in inhibiting Staphylococcus aureus colonization and biofilm formation. DeinoPol interferes with S. aureus biofilm formation by disrupting production of poly-N-acetylglucosamine, suggesting its potential application in preventing/treating S. aureus biofilm-related infections or inflammatory diseases.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Ching Hoong Chew, Chew Chieng Yeo, Ainal Mardziah Che Hamzah, Esra'a I. Al-Trad, Sherry Usun Jones, Kek Heng Chua, Suat Moi Puah
Summary: Multidrug resistance (MDR) poses a significant challenge in healthcare management, and a comprehensive approach is needed to address this issue. In this study, a combination of phenotypic and genotypic methods, including whole genome sequencing (WGS), was used to investigate hospital-associated MDR methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from newborn infants. The findings revealed different levels of resistance to antimicrobial classes among the MDR-MRSA strains, along with the presence of slime and biofilms, SCCmec type IV, and various types of microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) and virulence genes. The WGS data for specific strains showed the presence of antimicrobial resistance genes in the chromosomes, with one gene found in a small plasmid. The presence of MDR-MRSA strains in neonates raises public concern, and multifaceted interventions are recommended to address this issue. Furthermore, there is a need for metadata to improve the investigation of antimicrobial resistance genes in MDR isolates.
Article
Food Science & Technology
Haiyang Yan, Meng Li, Lingling Meng, Feng Zhao
Summary: The viable but nonculturable (VBNC) state of Staphylococcus aureus can be induced in citric acid buffer at -20 degrees C, causing changes in some biological characteristics, but can be successfully resuscitated under various conditions. The food industry should pay attention to the potential hazard posed by VBNC S. aureus under frozen conditions.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Microbiology
Shizhou Wu, Boquan Qin, Shu Deng, Yunjie Liu, Hui Zhang, Lei Lei, Guoying Feng
Summary: This study investigated the roles of YycF and CodY in biofilm formation and pathogenicity. The results revealed that YycF negatively regulates codY expression and can modulate S. aureus biofilm formation through eDNA- dependent and PIA-dependent pathways. CodY not only impedes the pathogenicity but also has a role in immunoregulation. These findings may provide a supplementary strategy for managing biofilm infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Jinxin Zheng, Yongpeng Shang, Yang Wu, Jianfeng Wu, Junwen Chen, Zhanwen Wang, Xiang Sun, Guangjian Xu, Qiwen Deng, Di Qu, Zhijian Yu
Summary: This study found that diclazuril had antibacterial action against both methicillin-sensitive and methicillin-resistant Staphylococcus aureus, inhibiting biofilm formation and hemolysis induced by the bacteria. The RNA levels of various genes related to biofilm formation and virulence were decreased after treatment with diclazuril, and mutations in target genes were detected in diclazuril nonsensitive clones of S. aureus.
ACS INFECTIOUS DISEASES
(2021)
Review
Biochemistry & Molecular Biology
Elibieta Cecerska-Heryc, Malgorzata Goszka, Natalia Serwin, Marta Roszak, Bartlomiej Grygorcewicz, Rafal Heryc, Barbara Dolegowska
Summary: Platelets produce growth factors that play a crucial role in wound healing. Platelet-rich plasma has been widely used in dentistry, sports medicine, dermatology, gynecology, ophthalmology, and neurology. However, further research is needed to better understand its potential and standardize its clinical application.
CYTOKINE & GROWTH FACTOR REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Natalia Kazmierczak, Bartlomiej Grygorcewicz, Marta Roszak, Beata Bochentyn, Lidia Piechowicz
Summary: The investigation and development of novel treatment strategies are necessary to address problems related to biofilm-related infections and antibiotic resistance. Bacteriophages, as promising alternatives to conventional antibiotics, have been shown to be effective antibiofilm agents in both in vitro and in vivo larva model studies. The results demonstrate that phages vB_SauM-A, vB_SauM-C, and vB_SauM-D can effectively reduce the colony-forming unit number of multidrug-resistant S. aureus strains in biofilms, independently of the phage titer used. Furthermore, bacteriophages were found to be more efficient than antibiotics in removing biofilm biomass and reducing staphylococci count. Additionally, phages vB_SauM-A, vB_SauM-C, and vB_SauM-D can enhance the survival rate and prolong the survival time of Galleria mellonella larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Elzbieta Cecerska-Heryc, Aleksandra Polikowska, Natalia Serwin, Marta Roszak, Bartlomiej Grygorcewicz, Rafal Heryc, Anna Michalczyk, Barbara Dolegowska
Summary: Oxidative stress refers to the persistent imbalance between the activity of toxic reactive forms of oxygen and nitrogen and the antioxidant defense. The brain is highly susceptible to oxidative imbalance due to its lipid-rich structure, high oxygen consumption, and lack of sufficient antioxidant protection. Oxidative stress has been linked to various psychiatric disorders and is characterized by behavioral, emotional, and cognitive abnormalities as well as significant impairment in social life.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Urology & Nephrology
Elzbieta Cecerska-Heryc, Rafal Heryc, Grazyna Dutkiewicz, Anna Michalczyk, Bartlomiej Grygorcewicz, Natalia Serwin, Sylwia Napiontek-Balinska, Barbara Dolegowska
Summary: This study found that the type of renal replacement therapy, age of patients, duration of dialysis, CKD causes, and stage of progression significantly affect the activity of XOR and its isoforms.
Article
Medicine, General & Internal
Natalia Serwin, Elzbieta Cecerska-Heryc, Ewa Pius-Sadowska, Karol Serwin, Anna Niedzwiedz, Magda Wisniewska, Marta Roszak, Bartlomiej Grygorcewicz, Edyta Skwirczynska, Boguslaw Machalinski, Barbara Dolegowska
Summary: The aim of this study was to evaluate the influence of asymptomatic infection and symptomatic COVID-19 on specific biochemical, renal, and immune parameters. It was found that asymptomatic infection leads to an increase in certain blood markers, similar to symptomatic COVID-19 patients.
Article
Biotechnology & Applied Microbiology
Maciej Konopacki, Bartlomiej Grygorcewicz, Marian Kordas, Paula Ossowicz-Rupniewska, Anna Nowak, Magdalena Peruzynska, Rafal Rakoczy
Summary: This study aimed to test the impact of a rotating magnetic field on bacterial cellulose production and develop a mathematical model for prediction. The results showed that the rotating magnetic field could stimulate the production of bacterial cellulose, and a properly selected forcing function could accurately predict the production.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Pawel Rynio, Katarzyna Galant, Lukasz Wojcik, Bartlomiej Grygorcewicz, Arkadiusz Kazimierczak, Aleksander Falkowski, Piotr Gutowski, Barbara Dolegowska, Milosz Kawa
Summary: Three-dimensionally-printed aortic templates are increasingly used in the treatment of urgent, complex aortic disorders. This study investigated the efficacy of different sterilization methods on these templates and found that high temperature sterilization caused deformation in some materials, while plasma and gas sterilization were effective for all tested printing materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Marta Roszak, Barbara Dolegowska, Elzbieta Cecerska-Heryc, Natalia Serwin, Joanna Jablonska, Bartlomiej Grygorcewicz
Summary: Alternative therapies against multidrug-resistant bacteria are widely investigated in the postantibiotic era. In this study, the effectiveness of combining bacteriophages with ciprofloxacin to combat Staphylococcus aureus in the presence of Candida albicans was examined. The results showed that the combination therapy led to the complete removal of S. aureus in liquid culture and a significant reduction in biofilm activity. The presence of C. albicans had an impact on the therapeutic effect.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Microbiology
Bartlomiej Grygorcewicz, Marta Roszak, Rafal Rakoczy, Adrian Augustyniak, Maciej Konopacki, Joanna Jablonska, Natalia Serwin, Elzbieta Cecerska-Heryc, Marian Kordas, Katarzyna Galant, Barbara Dolegowska
Summary: This study applied the PhageScore methodology to assess the activity of bacteriophage-antibiotic combination in liquid bacterial culture. The results showed that the addition of antibiotics had an impact on the lytic activity of bacteriophages. Therefore, the PhageScore method can be used as a tool for evaluating the synergy between bacteriophages and antibiotics.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Biochemical Research Methods
Elzbieta Cecerska-Heryc, Bartosz Ronkowski, Rafal Heryc, Natalia Serwin, Bartlomiej Grygorcewicz, Marta Roszak, Katarzyna Galant, Barbara Dolegowska
Summary: Proteomic and lipidomic studies have identified certain proteins and lipids that can be used for the diagnosis and treatment monitoring of inflammatory bowel disease (IBD). Analysis of protein and lipid profiles can improve the availability and use of diagnostic markers, enhance understanding of the disease mechanisms, aid in monitoring patient health, and improve therapeutic approaches.
PROTEOMICS CLINICAL APPLICATIONS
(2023)
Article
Microbiology
Maciej Konopacki, Joanna Jablonska, Kamila Dubrowska, Adrian Augustyniak, Bartlomiej Grygorcewicz, Marta Glizniewicz, Emil Wroblewski, Marian Kordas, Barbara Dolegowska, Rafal Rakoczy
Summary: Hydrodynamic conditions play a critical role in bioprocessing, affecting the availability of oxygen for cultured cells. This study characterized parameters such as mixing time, power consumption, and mass transfer in a 2 L bioreactor, and tested the hydrodynamic impact on a selected bioprocess. The results showed that high oxygen mass transfer and intensive mixing led to the highest pyocyanin production, while moderate oxygen mass transfer and less intense mixing resulted in maximal efficiency of rhamnolipid production.
Review
Cell Biology
Elzbieta Cecerska-Heryc, Maja Pekala, Natalia Serwin, Marta Glizniewicz, Bartlomiej Grygorcewicz, Anna Michalczyk, Rafal Heryc, Marta Budkowska, Barbara Dolegowska
Summary: Stem cells have shown great potential in treating neurological diseases such as MS, ALS, AD, PD, and HD. Different types of stem cells have demonstrated effectiveness in slowing down disease progression and improving symptoms. However, more research and refinement are needed to optimize the therapy's effectiveness in humans.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Chareza, Katarzyna Przygrodzka, Anna Zywicka, Bartlomiej Grygorcewicz, Peter Sobolewski, Sylwia Mozia, Marcin Smiglak, Radoslaw Drozd
Summary: Bacterial biofilms are a major problem in the wound healing process due to antibiotic resistance mechanisms. This study investigated the therapeutic properties of alginate lyase (AlgL) immobilised on BC membranes for protecting wounds from Pseudomonas aeruginosa infection. The AlgL immobilisation significantly reduced the amount of polysaccharides in the biofilm and showed synergism with gentamicin in killing bacterial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Bartlomiej Grygorcewicz, Marta Glizniewicz, Patrycja Olszewska, Dominika Milek, Artur Czajkowski, Natalia Serwin, Elzbieta Cecerska-Heryc, Rafal Rakoczy
Summary: This study explores the application of Response Surface Methodology (RSM) in optimizing the antibiofilm activity of phage-antibiotic combinations. By analyzing data from biofilm challenge assays using RSM, this method allows for the selection of optimal points of antibiofilm activity and provides quantifiable mathematical representations. The results suggest that synergistic effects can be achieved by judiciously combining antibiotics with bacteriophages, leading to a reduction in biofilm biomass of up to 80%, depending on the antibiotic used.
Article
Engineering, Chemical
Rafal Rakoczy, Maciej Konopacki, Marian Kordas, Bartlomiej Grygorcewicz
Summary: The production of bacteriophages involves two stages: analyzing the effects of different types of electromagnetic fields on the proliferation process of bacteria (host) and implementing the optimal infection conditions for bacteriophage production under the action of various types of electromagnetic fields. The study finds that the application of rotating magnetic field, pulsed magnetic field, and static magnetic field (negative polarity) is the most effective method for bacteriophage production.
CHEMICAL AND PROCESS ENGINEERING-INZYNIERIA CHEMICZNA I PROCESOWA
(2022)
